Ekstraksi DNA Phytophthora palmivora Isolat Kelapa

https://doi.org/10.22146/jpti.9358

Achmadi Priyatmojo(1*), Siti Subandiyah(2)

(1) Fakultas Pertanian Universitas Gadjah Mada
(2) Fakultas Pertanian Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Coconut isolate of Phytophthora palmivora DNA was extracted from sporangium and mycelium using SDS and Proteinase-K method. Sporangium and mycelium were harvested from liquid and solid culture of Potato Dextrose medium. The result of the study showed that the DNA extracted from sporangium and mycelium of 8 days solid culture was seen as distinct band. On the other hand, the dNA from sporangium and mycelium of 24 days old liquid culture was fragmented with smear band appearance.



Keywords


DNA; Phytophthora palmivora

Full Text:

PDF


References

Brahmana. J dan A. U. Lubis. 1995. Pengendalian terpadu penvakit busuk umbut basah Phyophthora palmivora di perkebunan kelapa. Risalah Kongres Nasional XII dan Seminar Ilmiah PFI. Yogyakarta. 1156 hal.

Clarkson. J.M 1992 Fungi, p. 67 - 78. In: Gun, S.J., M.J. McPherson, & D.J. Bowles (eds.), Molecular plant pathology volume I. Oxford Univ. Press. Oxford. London. 216 p.

Dirjenbun. 1992. Survei penyakit busuk pucuk (Phytophthora sp.) pada beberapa varietas kelapa di Indonesia. Direktorat Jenderal Perkebunan Departemen Pertanian. Jakarta. 35 hal.

Draper, John & R. Scott. 1988. The isolation of plant nucleid acids, p. 199 - 236. In : Draper, J., R. Scott, P. Armitage & R. Walden (eds.), Plant genetic transformation and gene expression. The Alden Press. Oxford. 355 p.

Erwin, D.C. 1983. Variability within and among species of Phytophthora. p. 149 - 165. In : Erwin, DC., S. Bartnicki-Garcia & P.H. Tsao (eds.), Phytophthora Its biology, taxonomy, ecology and pathology. APS., St. Paul. 187 p.

Gabriel, D.W. & R. de Feyter. 1992. RFLP analyses and gene tagging for bacterial identification and taxonomy, p. 51 - 66. In : Gurr, S.J., M.J. McPherson, & D.J. Bowles (eds.), Molecular plant pathology volume 1. Oxford Univ. Press. Oxford. London. 216 p.

Gurr, S.J. & M.J. McPherson. 1992. Nucleid acid isolation and hybridization techniques. p. 109 - 122. In : Gurr, S.J., M.J. McPherson, & D.J. Bowles (eds.). Molecular plant pathology volume I. Oxford Univ. Press. Oxford. London. 216 p.

Kirby, L.T. 1990. DNA fingerprinting an introduction. M. Stockton Press. New York. 365 p. [9] McPherson, M.J., R.J. Oliver & S.J. Gurr. The polymerase chain reaction, p. 123 - 146. In : Gurr, S.J., M.J. McPherson, & D.J. Bowles (eds.). Molecular plans pathology volume I. Oxford Univ. Press. Oxford. London. 216 p.

Semangun, Haryono. 1988. Penyakit-penyakit tanaman perkebunan di Indonesia. Gadjah Mada Press. Yogyakana. 808 hal.

Waterhouse, G.M.. F.J. Newhook, and D.J. Stamps. 1983. Present criteria for classification of Phytophthora. p. 139 - 147. In : Erwin. DC., S. Bartnicki-Garcia & P.H. Tsao (eds.), Phytophihora Its biology, taxonomy. ecology and pathology. APS. St. Paul. 187 p.

Zentmyer, G.A. 1974. Variation, genetics, and geographical distribution of mating types. p. 89 - 101. In : Gregory, P.H (ed.). Phytopthora disease of cocoa. Longman Group Limited. London.

Zentmyer, GA. 1983. The world of Phytophthora. p. 1 - 9. In : Erwin, DC., S. Bartnicki-Garcia & PH. Tsao (eds.), Phytophthora its biology. Taxonomy, ecology and pathology. APS.St. Paul. l87p.



DOI: https://doi.org/10.22146/jpti.9358

Article Metrics

Abstract views : 1342 | views : 1257

Refbacks

  • There are currently no refbacks.




Copyright (c) 1996 Jurnal Perlindungan Tanaman Indonesia

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print), ISSN 2548-4788 (online) is published by the Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.  

View website statistics