* Corresponding Author
Evaluation of Three Cucurbitaceae Cultivars Resistant against Begomoviruses Based on Morphological Symptoms and Molecular Analysis
https://doi.org/10.22146/jpti.87915 Aprilia Sufi Subiastuti(1), Muhammad Syafi’atol Huda(2), Budi Setiadi Daryono(3*)
(1) Laboratory of Microbiology, Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Jln. Teknika Selatan, Sekip Utara, Sleman, Yogyakarta 55281 Indonesia
(2) Laboratory of Genetic and Breeding, Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Jln. Teknika Selatan, Sekip Utara, Sleman, Yogyakarta 55281 Indonesia
(3) Laboratory of Genetic and Breeding, Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Jln. Teknika Selatan, Sekip Utara, Sleman, Yogyakarta 55281 Indonesia
(*) Corresponding Author
Abstract
Cucurbitaceae is a group of annual horticultural crops with high economic prospects and benefits. The Faculty of Biology, Universitas Gadjah Mada has developed several new cultivars within the Cucurbitaceae family, such as the melon cultivar 'Hikapel', watermelon 'Citra Jingga', and butternut squash 'Citra Laga'. However, development of these cultivars has been hindered by yellow disease, typically triggered by Begomovirus infection. This study aimed to determine the morphological symptoms of yellow disease, to detect Begomovirus infection using molecular approach, and assess resistance levels of the three Cucurbitaceae cultivars. The method involved observing morphological symptoms in plants and measuring plant height. The degree of symptom severity was then converted into a vulnerability index (VI). The symptomatic leaf samples were tested using Krusty and Homer primer to confirm the symptoms caused by Begomovirus. The PCR results were sequenced and analyzed using Maximum Likelihood method in MEGA 11. The results showed that symptoms included curling tip of leaves, yellow mosaic on the leaves, and stunted growth in some plants, which were suggested as Begomovirus infection. Based on the Vulnerability Index (VI), the watermelon cultivar 'Hikapel' was considered susceptible to Begomovirus infection (VI = 85%), while the yellow squash 'Citra Laga' was moderately susceptible (VI = 57.5%). However, the watermelon 'Citra Jingga' was considered moderately resistant to this infection (VI = 50%). The presence of DNA the Begomovirus at three species Cucurbitaceae were characterized by a 550 bp band. The 'Hikapel' melon and 'Citra Laga' butternut were infected with Tomato leaf curl New Delhi virus (ToLCNDV), while the ‘Citra Jingga’ watermelon were infected with Squash leaf curl Philippine virus (SLCuPV).
Keywords
Full Text:
PDFReferences
Badan Pusat Statistik. (2022). Hortikultura. https://www.bps.go.id/subject/55/ hortikultura.html
Brown, J.K., Zerbini, F.M., Navas-Castillo, J., Moriones, E., Ramos-Sobrinho, R., Silva, J.C.F., . . . Varsani, A. (2015). Revision of Begomovirus Taxonomy Based on Pairwise Sequence Comparisons. Archives of Virology, 160(6), 1593‒1619. https://doi.org/10.1007/s00705-015-2398-y
Friedmann, M., Lapidot, M., & Pillowsky, M. (1998). A Novel Source of Resistance to Tomato Yellow Leaf Curl Virus Exhibiting a Symtompless Reaction to Viral Infection. Journal of the American Society for Horticultural Science, 123(6), 1004‒1007. https://doi.org/10.21273/JASHS.123.6.1004
Haerunisa, R., Suastika, G., & Damayanti, T.A. (2016). Identifikasi Begomovirus yang Berasosiasi dengan Penyakit Kuning pada Mentimun di Jawa Barat dan Bali. Jurnal Hortikultura Indonesia, 7(1), 9–20. https://doi.org/10.29244/jhi.7.1.9-20
Hafizh, A. (2021). Ketahanan Semangka (Citrullus lanantus (Thunb.) Matsum & Nakai) terhadap Infeksi Begomovirus. [Undergraduate Thesis]. Yogyakarta, Indonesia: Fakultas Biologi Universitas Gadjah Mada.
Hull, R. (2009). Comparative Plant Virology (2nd ed.). Elsevier/Academic Press.
Islam, S., Das, M.A., Verma, M., Arya, L., Mandal, B., Kanti, B.T., . . . Kumar, L.S. (2011). Screening of Luffa cylindrica Roem. for Resistance against Tomato Leaf Curl New Delhi Virus, Inheritance of Resistance, and Identification of SRAP Markers Linked to the Single Dominant Resistance Gene. The Journal of Horticultural Science & Biotechnology, 86(6), 661‒667. https://doi.org/10.1080/14620316.2011.11512819
Kementerian Pertanian Republik Indonesia. (2019). Surat Keputusan Menteri Pertanian Republik Indonesia nomor 19/PV.220/A9/01/2019. p.1, Lampiran.
Kesumawati, E., Okabe, S., Khalil, M., Alfan, G., Bahagia, P., Pohan, N., . . . Koeda, S. (2020). Molecular Characterization of Begomoviruses Associated with Yellow Leaf Curl Disease in Solanaceae and Cucurbitaceae Crops from Northern Sumatra, Indonesia. The Horticulture Journal, 89(4), 410‒416. https://doi.org/10.2503/hortj.UTD-175
Kundu, A., Singh, P.K., Dey, A., Ganguli, S., & Pal, A. (2019). Complex Molecular Mechanisms Underlying MYMIV-Resistance in Vigna mungo Revealed by Comparative Transcriptome Profling. Science Report, 9, 8858. https://doi.org/10.1038/s41598-019-45383-w
Kurnia, T.D., Purwantoro, A., Sulandari, S., Basunanda, P., Setiawan, A.B., Fatmawati, Y., & Andika, I.P. (2022). Molecular and Morpho-physiological Identification of Yellow Leaf Curl Disease of Cucumber in Salatiga, Indonesia. Biodiversitas, 23(3), 1466‒1474. https://doi.org/10.13057/biodiv/d230334
Kusumaningrum, F., Hartono, S., Sulandari, S., & Somowiyarjo, S. (2015). Infeksi Ganda Begomovirus dan Crinivirus pada Tanaman Tomat di Kabupaten Magelang, Jawa Tengah. Jurnal Perlindungan Tanaman Indonesia, 19(2), 60–64. https://doi.org/10.22146/jpti.17542
Lestari, S.M., Hidayat, S.H., Hidayat, P., Kil, E.J., Lee, S., Kim, S.M., & Lee, K.Y. (2023). Identification of Begomoviruses Associated with the Insect Vector Bemisia tabaci and Various Host Plants on Java Island, Indonesia. Archives of Insect Biochemistry and Physiology, 12(2), e21984. https://doi.org/10.1002/arch.21984
Nopianasanti, H., & Daryono, B.S. (2018). Kestabilan Fenotip Tanaman Labu Susu (Cucurbita moschata (Duchesne) Poir “Butternut”) Hasil Budidaya di Sleman, D.I. Yogyakarta. Biogenesis, 6(2), 115‒123. https://doi.org/10.24252/bio.v6i2.4751
Ogbe, F.O., Atiri, G.I., Dixon, A.G.O., & Thottappilly, G. (2003). Symptom Severity of Cassava Mosaic Disease in Relation to Concentration of African cassava mosaic virus in Different Cassava Genotypes. Plant Pathology, 52(1), 84–91. https://doi.org/10.1046/j.1365-3059.2003.00805.x
Paul, S., Ghosh, R., Roy, A., & Ghosh, S.K. (2012). Analysis of Coat Protein Gene Sequences of Begomoviruses Associated with Different Weed Species in India. Phytoparasitica, 40(1), 95–100. https://doi.org/10.1007/s12600-011-0202-4
Paryadi, S. & Hadiatna, E. (2021). Budidaya Tanaman Melon. Sleman, Indonesia: Deepublish Publisher.
Revill, P.A., Ha, C.V., Porchun, S.C., Vu, M.T., & Dale, J.L. (2003). The Complete Nucleotide Sequence of Two Distinct Geminiviruses Infecting Cucurbits in Vietnam. Archives of Virology, 148, 1523‒1541. https://doi.org/10.1007/s00705-003-0109-6
Reuveni, M., Debbi, A., Kutsher, Y., Gelbart, D., Zemach, H., Belausov, E., . . . Lapidot, M. (2015). Tomato yellow leaf curl virus Effects on Chloroplast Biogenesis and Cellular Structure. Physiological and Molecular Plant Pathology, 92, 51‒58. https://doi.org/10.1016/j.pmpp.2015.08.001
Rojas, M.R., Gilbertson, R.L., Russell, D.R., & Maxwell, D.P. (1993). Use of Degenerate Primers in the Polymerase Chain Reaction to Detect Whitefly-transmitted Geminiviruses. Plant Disease, 77(4), 340‒347. https://doi.org/ 10.1094/PD-77-0340
Santoso, T.J., Hidayat, S.H., Ati, S.D., Muhammad, H., & Sudarsono. (2008). Identity and Sequence Diversity of Begomovirus Associated with Yellow Leaf Curl Disease of Tomato in Indonesia. Microbiology Indonesia, 2(1), 1‒7. https://doi.org/10.5454/mi.2.1.1
Saxena, S. & Tiwari, A.K. (2017). Begomoviruses: Occurrence and Management in Asia and Africa. Singapore: Springer Nature Pte. Ltd. https://doi.org/10.1007/978-981-10-5984-1
Selangga, D.G.W., & Listihani. (2022). Squash Leaf Curl Virus: Species of Begomovirus as the Cause of Butternut Squash Yield Losses in Indonesia. Hayati Journal of Biosciences. 29(6), 806‒813. https://doi.org/10.4308/hjb.29.6.806-813
Subiastuti, A.S. (2015). Deteksi Gen Ketahanan terhadap Begomovirus dan Analisis Kandungan Nutrisi pada Melon (Cucumis melo L. cv. Hikapel) [Undergraduate Thesis]. Yogyakarta, Indonesia: Fakultas Biologi Universitas Gadjah Mada.
Subiastuti, A.S., Hartono, S., & Daryono, B.S. (2019). Detection and Identification of Begomovirus Infecting Cucurbitaceae and Solanaceae in Yogyakarta, Indonesia. Biodiversitas, 20(3), 738‒744. https://doi.org/10.13057/biodiv/d200318
Sufrin-Ringwald, T., & Lapidot, M. (2010). Characterization of A Synergistic Interaction Between Two Cucurbit-Infecting Begomoviruses: Squash Leaf Curl Virus and Watermelon Chlorotic Stunt Virus. The American Phytopathology Society, 101(2), 281‒289. https://doi.org/10.1094/PHYTO-06-10-0159
Trisno, J., Hidayat, S.H., Trimukti, H., Ishak, M., & Jamsari, J. (2009). Detection and Sequence Diversity of Begomovirus Assosiated with Yellow Leaf Curl Disease of Pepper (Capsicum annuum) in West Sumatra, Indonesia. Microbiology Indonesia, 3(2), 56‒61. https://doi.org/10.5454/mi.3.2.2
Wang, A. (2015). Dissecting the Molecular Network of Virus-Plant Interactions: The Complex Roles of Host Factors. Annual Review of Phytopathology, 53(1), 45–66. https://doi.org/10.1146/annurev-phyto-080614-120001
Wilisiani, F., Somowiyarjo, S., & Hartono, S. (2014). Identifikasi Molekuler Virus Penyebab Penyakit Daun Keriting Isolat Bantul pada Melon. Jurnal Perlindungan Tanaman Indonesia, 18(1), 47–54. Retrieved from https://jurnal.ugm.ac.id/jpti/article/view/15602
Wiratama, I.D.P., Gusti, N.A.W., Ni-Nengah, P.A., Dewa, N.N., & Gede, S. (2015). Temuan Penyakit Baru Laporan Pertama Infeksi Begomovirus pada Tanaman Mentimun di Bali. Jurnal Fitopatologi Indonesia, 11(5), 175–178. https://doi.org/10.14692/jfi.11.5.175
Wyatt, S., & Brown, J.K. (1996) Detection of Subgroup III Gemini-virus Isolates in Leaf Extracts by Degenerate Primers and Polymerase Chain Reaction. Phytopathology, 86(12), 1288–1293. https://doi.org/10.1094/Phyto-86-1288
DOI: https://doi.org/10.22146/jpti.87915
Article Metrics
Abstract views : 566
| 
views : 155
Refbacks
- There are currently no refbacks.
Copyright (c) 2024 Jurnal Perlindungan Tanaman Indonesia
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print), ISSN 2548-4788 (online) is published by the Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
View website statistics