In Vitro Evaluation on Resistance of Phytopythium vexans (NG Isolate) Cultured from Sublethal Concentration against Several Fungicides

https://doi.org/10.22146/jpti.73045

Islaminati Anna Santika(1), Arif Wibowo(2), Suryanti Suryanti(3), Christanti Sumardiyono(4), Ani Widiastuti(5*)

(1) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Jln. Flora 1. Bulaksumur, Sleman, Yogyakarta, 55281 Indonesia
(2) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Jln. Flora 1. Bulaksumur, Sleman, Yogyakarta, 55281 Indonesia
(3) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Jln. Flora 1. Bulaksumur, Sleman, Yogyakarta, 55281 Indonesia
(4) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Jln. Flora 1. Bulaksumur, Sleman, Yogyakarta, 55281 Indonesia
(5) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Jln. Flora 1. Bulaksumur, Sleman, Yogyakarta, 55281 Indonesia
(*) Corresponding Author

Abstract


Phytopythium vexans isolated from potatoes rhizospher in Ngablak, Magelang, Central Java was reported having a potency to cause potato tuber rot in in vitro test. Some fungicides had been intensively applied in this area to control potato late blight even using sublethal dose. This study investigated resistance response of the pathogen against several fungicides which were Mancozeb, Dimethomorph, mixture of Mancozeb and Mefenoxam, and mixture of Oxathiapiprolin and Famoxadone. The experiment was conducted to observe the sensitivity response of pathogen against those fungicide and the experiment repeated with one of the isolates which growth in sublethal concentration to observe the resistance response. Both experiment data was analyzed by Probit Analysis. On sentivity test, data showed that P. vexans were insensitive against Dimethomorph, but it was sensitive against Mancozeb, mixture of Mancozeb and Mefenoxam, as well as mixture of Oxathiapiprolin and Famoxadone. On Mancozeb treatment, mycelia were inhibited 100% at the sugested concentration. However, at the sublethal concentration the mycelial collony grew abnormally. Pathogen isolate from the sublethal concentration were used as material for the resistance response study. The effect of the sublethal application of Mancozeb to P. vexans caused resistance to Mancozeb itself and cross resistance against Dimetomorph and mixture of Oxathiapiprolin and Famoxadone. It was proven by higher value of predicted EC50 and EC95 on the second experiment compared to the predicted EC50 and EC95 from the first one. The resistant P. vexans isolate was able to produce sporangium and chlamydospore.

Keywords


EC50; Phytopythium vexans; potato; resistance

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References

Amaradasa, B.S., & Everhart, S.E. (2016). Effects of Sublethal Fungicides on Mutation Rates and Genomic Variation in Fungal Plant Pathogen, Sclerotinia sclerotiorum. PLoS ONE, 11(12), e0168079. https://doi.org/10.1371/journal.pone.0168079

Centre for Agriculture and Biosciences International [CABI]. (2019). Pythium vexans. Retrieved from http://www.cabi.org/isc/datasheet/46174#39E96248-57FF-4966-B558-A8639528B2B8

Cohen, Y., Rubin, A.E., & Galperin, M. (2018). Oxathiapiprolin-based Fungicides Provide Enhanced Control of Tomato Late Blight Induced by Mefenoxam-insensitive Pythopthora infestans. PLoS ONE, 13(9), e0204523. https://doi.org/10.1371/journal.pone.0204523

Corio-Costet, M.F. (2011). Fungicide Resistance in Plasmopara viticola in France and Anti-resistance Measures. In T.S. Thind (Ed.), Fungicide Resistance in Crop Protection Risk and Management (pp. 157‒171). Retrieved from https://www.cabdirect.org/cabdirect/abstract/20113397636

Fungicide Resistance Action Committee [FRAC]. (2020). Fungal Control Agents Sorted by Cross Resistance Pattern and Mode of Action. Retrieved from https://cpb-us-w2.wpmucdn.com/u.osu.edu/dist/b/28945/files/2020/02/frac-code-list-2020-final.pdf

Fungicide Resistance Action Committee [FRAC]. (2018). FRAC Code List: Fungicides Sorted by Mode of Action. Retrieved from http://www.phi-base.org/images/fracCodeList.pdf

Kuhn, P.J., Pitt, D., Lee, S.A., Wakley, G., & Sheppard, A.N. (1991). Effect of Dimethomorph on the Morphology and Ultrastructure of Phytophthora. Mycology Resources, 98(3), 333-340.

Levesque, C.A., & De Cock, W.A.M. (2008). Separation of Pythium Taxa Using Nuclear and Mitochondrial DNA Markers: Proposal of a New Genus, Phytopythium gen. nov. Retrieved from https://www.isppweb.org› 3rd_International Oomycetes Workshop 2008

Luo, Y., Yao, A., Tan, M., Li, Z., Qing, L., & Yang, S. (2020). Effects of Manganese and Zinc on the Growth Process of Phytophthora nicotianae and the Possible Inhibitory Mechanisms. PeerJ, 8, e8613. https://doi.org/10.7717/peerj.8613

Malandakris, A.A., Apostolidou, Z.A., Markoglou, A., & Flouri, F. (2015). Fitness and Cross-resistance of Alternaria alternata Field Isolates with Specific or Multiple Resistance to Single Site Inhibitors and Mancozeb. European Journal of Plant Pathology, 142(3), 480-499.

Manalu, J.N., Soekarno, B.P.W., Tondok, E.T., & Surono, S. (2020). Isolation and Capability of Dark Septate Endophyte against Mancozeb Fungicide. Jurnal Ilmu Pertanian Indonesia, 25(2), 193-198. https://doi.org/10.18343/jipi.25.2.193

Mazáková, J.M. Zouhar, P. Ryšánek, V. Táborský, E. H. ater, & Petr Doležal. (2011). Sensitivity to Fungicides in the Isolates of Phytophthora infestans (Mont.) de Bary in the Czech Republic from 2003 to 2008. Journal of Plant Protection Science, 47(1), 5-12.

Miao, J.Q., Meng C., Xue D., Li L., Dong L., Can Z., Zhili P. & Xili L. (2016). Resistance Assessment for Oxathiapripolin in Phytophthora capsici and the Detection of a Point Mutation (G769W) in PcORP1 that Confers Resistance. Frontiers in Microbiology, 7 (615), 1-14.

Polat, Z., Awan, Q.N., Hussain, M., & Akgul, D.S. (2017). First Report of Phytopythium vexans Causing Root and Collar Rot of Kiwifruit in Turkey. Plant Disease, 101(6), 1058. https://doi.org/10.1094/PDIS-11-16-1554-PDN

Rudsari, M.K., S. M. Okhovat, M. Mirabolfathi, E. Jones, & Mohsen Kafi. 2015. Identification of Pythium species and their pathogenicity on cool season turfgrass in Tehran province. Biological Forum – An International Journal 7(2): 349-356.

Senhaji, D. Ben Hmamou, R. Salghi, A. Zarrouk, B. Chebli, H. Zarrok, I. Warad, B. Hammouti, & S. S. Al-Deyab. (2013). Asteriscus Imbricatus Extracts: Antifungal Activity and Anticorrosion Inhibition B. International Journal Electrochemical Science, 8, 6033–6046.

Santoso, P.J. (2016). Karakteristik Empat Cendawan Patogen pada Durian: Phytophthora palmivora, Phytopythium vexans, Pythium cucurbitacearum, dan Pythium sp. D37. IPTEK Hortikultura, (12), 59-64.

Santika, I.A., Widiastuti, A., & Wibowo, A. (2021). First Report of Phytopythium vexans (de Barry) Abad, de Cock, Bala, Robideau, Lodhi & Lévesque Causing Potato Tuber Rot in Indonesia. Jurnal Perlindungan Tanaman Indonesia, 25(2), 173-181.

Sumardiyono, C. (2008). Ketahanan Jamur terhadap Fungisida di Indonesia. Jurnal Perlindungan Tanaman Indonesia, 14(1), 1-5.

Yu, Y.T., Chen J., Gao C.S Zeng L.B., Li Z.M., Zhu T.T., Sun K., Cheng Y., Sun X.P., Yan L., Yan Z., & Zhu A. (2016). First Report of Brown Root Rot Caused by Pythium vexans on Ramie in Hunan, China. Canadian Journal of Plant Pathology, 38 (3), 405-410.



DOI: https://doi.org/10.22146/jpti.73045

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