Double Infections of Rehmannia mosaic virus and Potato virus Y on Tobacco Plants in Central Java and Special Region of Yogyakarta

https://doi.org/10.22146/jpti.67468

Tika Pramudya Wardani(1), Sedyo Hartono(2*), Sri Sulandari(3), Susamto Somowiyarjo(4)

(1) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281 Indonesia
(2) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281 Indonesia
(3) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281 Indonesia
(4) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281 Indonesia
(*) Corresponding Author

Abstract


Various viruses can cause mosaic disease on tobacco plants. Proper detection of the cause of the mosaic disease helps determine effective control. The purpose of this study was to detect the presence of double infection of Rehmannia mosaic virus (ReMV) with Potato virus Y (PVY) using multiplex RT-PCR in tobacco plants from Central Java and Special Region of Yogyakarta. The viral suspension was inoculated on Chenopodium amaranticolor to obtain one viral colony from one local lesion. The multiplex RT-PCR method using Tobamovirus primers (TobRT-up1 and TobRT-do2) and Potyvirus primers (MJ1 and MJ2) can detect double infection caused by ReMV with PVY in tobacco plants distributed in Central Java and Special Region of Yogyakarta. The multiplex RT-PCR product showed that tobacco samples with mosaic symptoms from Temanggung, Klaten, Bantul, and Kalasan were positive ReMV. Multiplex RT-PCR has successfully detected double infection of ReMV and PVY on tobacco samples from Klaten and Kalasan. ReMV Bantul, Kalasan, and Klaten were homolog to ReMV USA isolate and ReMV Temanggung was homolog to ReMV Japanese isolate. PVY Klaten was homolog to PVY Turkey isolate, and PVY Kalasan was homolog to PVY Iran. 


Keywords


double infection; multiplex RT-PCR; Nicotiana tabacum; Potato virus Y; Rehmannia mosaic virus

Full Text:

PDF


References

Abdullah, A., Sativa, H.A., Nurhayati, T., & Nurilmala, M. (2019). Pemanfaatan DNA Barcoding untuk Ketertelusuran Label Berbagai Produk Olahan Ikan Berbasis Surimi Komersial. Jurnal Pengolahan Hasil Perikanan Indonesia, 22(3), 508–519. https://doi.org/10.17844/jphpi.v22i3.28950

Asharo, R.K., Manuhara, Y.S.W., Wahyuningsih, S.P.A., & Darsono, N. (2017). Identification of RNA Viruses Causing Sugarcane (Saccharum officinarum L.) Mosaic Disease by Simultaneously Multiplex-RT-PCR. Bioscience Research, 14(3), 446–454. Retrieved from http://isisn.org/BR-14-2017/446-454-14(3)2017BR-1473.pdf

Balogun, O.S., Xu, L., Teraoka, T., & Hosokawa, D. (2002). Effects of Single and Double Infections with Potato virus X and Tobacco mosaic virus on Disease Development, Plant Growth and Virus Accumulation in Tomato. Fitopatologia Brasileira, 27(3), 241–248. https://doi.org/10.1590/S0100-41582002000300001

Chatzivassiliou, E.K., Efthimiou, K., Drossos, E., Papadopoulou, A., Poimenidis, G., & Katis, N.I. (2004). A Survey of Tobacco Viruses in Tobacco Crops and Native Flora in Greece. European Journal of Plant Pathology, 110(10), 1011–1023. https://doi.org/10.1007/s10658-004-1598-1

Dalmadiyo, G., Supriyono, & Adi, B.H. (1997). Monograf Tembakau Virginia: Penyakit Tanaman Tembakau Virginia dan Pengendaliannya. Malang, Indonesia: Balai Penelitian Tembakau dan Tanaman Serat.

Direktorat Jenderal Perkebunan. (2021). Produksi Tembakau Menurut Provinsi di Indonesia, 2016--2020. Retrieved from https://www.pertanian.go.id/home/index.php?show=repo&fileNum=207

Dovas, C.I., Efthimiou, K., & Katis, N.I. (2004). Generic Detection and Differentiation of Tobamoviruses by A Spot Nested RT-PCR-RFLP Using dG-Containing Primers Along with Homologous dG-Containing Primers. Journal of Virological Methods, 117(2), 137–144. https://doi.org/10.1016/j.jviromet.2004.01.004

Korbecka-Glinka, G., Czubacka, A., Przybys, M., & Doroszewska, T. (2017). Resistance vs. Tolerance to Potato virus Y in Tobacco—Comparing Effectiveness Using Virus Isolates from Central Europe. Breeding Science, 67(5), 459–465. https://doi.org/10.1270/jsbbs.17019

Hall, T.A. (1999). Bioedit: A User-friendly Biological Sequence Alignment Editor and Analysis Program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98.

Hanadyo, R., Hadiastono, T., & Martosudiro, M. (2013). Pengaruh Pemberian Pupuk Daun Cair terhadap Intensitas Serangan Tobacco mosaic virus (TMV), Pertumbuhan dan Produksi Tanaman Tembakau (Nicotiana tabacum L.). Jurnal HPT (Hama Penyakit Tumbuhan), 1(2), 28–36. Retrieved from http://jurnalhpt.ub.ac.id/index.php/jhpt/article/view/15

International Committee on Taxonomy of Viruses. (2021a). Positive-sense RNA Viruses-Virgaviridae Genus: Tobamovirus. Retrieved from https://talk.ictvonline.org/ictv-reports/ictv_online_report/positive-sense-rna-viruses/w/virgaviridae/672/genus-tobamovirus

International Committee on Taxonomy of Viruses. (2021b). Potyviridae. Retrieved from https://talk.ictvonline.org/ictv-reports/ictv_online_report/positive-sense-rna-viruses/w/potyviridae#Species

Kubota, K., Usugi, T., Tomitaka, Y., Matsushita, Y., Higashiyama, M., Kosaka, Y., & Tsuda, S. (2012). Characterization of Rehmannia mosaic virus Isolated from Chili Pepper (Capsicum annuum) in Japan. Journal of General Plant Pathology, 78(1), 43–48. https://doi.org/10.1007/s10327-011-0345-7

Kumar, S., Stecher, G. & Tamura, K. (2016). MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Molecular Biology and Evolution, 33(7), 1870–1874. https://doi.org/10.1093/molbev/msw054

Marie-Jeanne, V., Ioos, R., Peyre, J., Alliot, B., & Signoret, P. (2000). Differentiation of Poaceae Potyviruses by Reverse Transcription Polymerase Chain Reaction and Restriction Analysis. Journal of Phytopathology, 148(3), 141–151.

Mochizuki, T., & Ohki, S.T. (2012). Cucumber mosaic virus: Viral Genes as Virulence Determinants. Molecular Plant Pathology, 13(3), 217–225.

Ningrum, E.P., Hartono, S., Sulandari, S., & Somowiyarjo, S. (2019). Multiplex RT-PCR Assay for Crinivirus Detection Using RNA Prepared from Three Extraction Methods on Tomato Plant. Jurnal Perlindungan Tanaman Indonesia, 23(2), 250–260. https://doi.org/10.22146/jpti.36558

Otsuki, Y., & Takebe, I. (1976). Double Infection of Isolated Tobacco Mesophyll Protoplasts by Unrelated Plant Viruses. Journal of General Virology, 30(3), 309–316.

Shi, X., Gao, Y., Yan, S., Tang, X., Zhou, X., Zhang, D., & Liu, Y. (2016). Aphid Performance Changes with Plant Defense Mediated by Cucumber mosaic virus Titer. Virology Journal, 13, 70. https://doi.org/10.1186/s12985-016-0524-4

Shukla, D., & Ward, C.W. (1988). Amino Acid Sequence Homology of Coat protein as a Basis for Identification and Classification of the Potyvirus Group. Journal of General Virology, 69(11), 2703–2710.

Tian, Y.P., Liu, J.L., Zhang, C.L., Liu, Y.Y., Wang, B., Li, X.-D., Guo, Z.K., & Valkonen, J.P.T. (2011). Genetic Diversity of Potato virus Y Infecting Tobacco Crops in China. Phytopathology, 101(3), 377–387. https://doi.org/10.1094/PHYTO-02-10-0055

Tsedaley, B. (2015). A Review Paper on Potato Virus Y (PVY) Biology, Economic Importance and its Managements. Journal of Biology, Agriculture and Healthcare, 5(9), 110–126. Retrieved from https://www.iiste.org/Journals/index.php/JBAH/article/view/22323

Wylie, S., Wilson, C.R., Jones, R.A.C., & Jones, M.G.K. (1993). A Polymerase Chain Reaction Assay for Cucumber mosaic virus in Lupin Seeds’. Australian Journal of Agricultural Research, 44(1), 41–51. https://doi.org/10.1071/AR9930041

Zhang, Z.C., Lei, C.Y., Zhang, L.F., Yang, X.X., Chen, R., & Zhang, D.S. (2008). The Complete Nucleotide Sequence of a Novel Tobamovirus, Rehmannia mosaic virus. Archives of Virology, 153, 595–599. https://doi.org/10.1007/s00705-007-0002-9

Zhang, Z.C., Zhang, L.F., Qiao, Q., Wang, Y.J., & Jin, X.L. (2004). Identification of Viral Pathogens of Rehmannia Glutinosa Disease in Henan Province. Acta Phytopathologica Sinica, 34(5), 395–399.



DOI: https://doi.org/10.22146/jpti.67468

Article Metrics

Abstract views : 417 | views : 185

Refbacks

  • There are currently no refbacks.




Copyright (c) 2021 Jurnal Perlindungan Tanaman Indonesia

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

 height=auto

Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print)ISSN 2548-4788 (online) is published by Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.  

View website statistics