Molecular Identification of Sweet potato virus C on Sweetpotato in Bali, Indonesia

https://doi.org/10.22146/jpti.64545

Listihani Listihani(1), Dewa Gede Wiryangga Selangga(2*)

(1) Faculty of Agriculture and Business, University of Mahasaraswati Denpasar Jln. Kamboja No.11 A, Dangin Puri Kangin, Denpasar Utara, Bali 80233 Indonesia
(2) Faculty of Agriculture, Warmadewa University Jln. Terompong No. 24, Tanjung Bungkak, Denpasar, Bali 80239 Indonesia
(*) Corresponding Author

Abstract


A survey was conducted in several sweet potato cultivations in Bali Province. Survey found that many plants exhibited potyvirus symptom, such as chlorosis blotches. This study was to determine disease incidence, detection and identification of the virus causing these symptoms on sweet potato plants in Bali. Samples were collected by purposive sampling of 10 plants from each location in Bali (Denpasar, Gianyar, Badung, Buleleng, Tabanan, Klungkung, Karangasem, Jembrana, Bangli). Disease insidence was observed based on viral symptoms in the field. Identification of nucleic acids was done using Potyvirus universal primer and DNA sequencing. Disease incidence in Bangli, Buleleng, and Denpasar Regencies was > 50%. RT-PCR and CiFor/CiRev Potyvirus universal primers successfully amplified ± 700 bp of CI genes from all samples from Bangli, while samples from 8 other districts were not amplified using the same primers. The SPVC isolate of sweet potato showed nucleotide and amino acid homology similarities with the sweet potato isolate from East Timor (MF572066), 96.8% and 97.4%, respectively and these were referred to the "Asian" strain. This indicates that SPVC has spread in East Java and Bali.


Keywords


chlorosis blot symptoms; identification; Potyvirus; SPCV Bali isolates

Full Text:

PDF


References

Abad, J.A., Parks, E.J., New, S.L., Fuentes, S., Jester, W., & Moyer, J.W. (2007). First Report of Sweet potato chlorotic stunt virus (Genus Crinivirus, Family Closteroviridae) in North Carolina. Plant Disease, 91(3), 327. https://doi.org/10.1094/PDIS-91-3-0327B

Adams, M.J., Antoniw, J.F. & Fauquet, C.M. (2005). Molecular Criteria for Genus and Species Discrimination within the Family Potyviridae. Archives of Virology, 150(3), 459–479. https://doi.org/10.1007/s00705-004-0440-6

Albuquerque, L.C., Inoue-Nagata, A.K., Pinheiro, B., S. Ribeiro, R.O. Resende, E. Moriones, & J. Navas-Castillo. (2011). A Novel of Monopartite Begomovirus Infecting Sweet Potato in Brazil. Archives of Virology, 156(7), 1291–1294. https://doi.org/10.1007/s00705-011-1016-x

Anjarsari, L., Suastika, G., & Damayanti, T.A. (2013). Deteksi dan Identifikasi Potyvirus pada Ubi Jalar di Tana Toraja, Sulawesi Selatan. Jurnal Fitopatologi Indonesia, 9(6), 193–201. https://doi.org/10.14692/jfi.9.6.193

Badan Pusat Statistik. (2017). Produksi Tanaman Hortikultura di Indonesia Tahun 2013–2017. Jakarta, Indonesia.

Clark, C.A., Davis, J.A., Abad, J.A., Cuellar, W.J., Fuentes, S., Kreuze, J.F., ... Valkonen, J.P. (2012). Sweet Potato Viruses: 15 Years of Progress on Understanding and Managing Complex Diseases. Plant Disease, 96(2), 168–185. https://doi.org/10.1094/PDIS-07-11-0550

Damayanti, T.A., Hondo, A., & Prayoga, Y. (2019). Infeksi Alami Pepper Yellow Leaf Curl Virus dan Sweet potato virus C pada Ubi Jalar di Malang, Jawa Timur. Jurnal Fitopatologi Indonesia, 15(6), 248–254. https://doi.org/10.14692/jfi.15.6.248-254

Doyle, J.J., & Doyle, J.L. (1987). A Rapid DNA Isolation Procedure for Small Quantities of Fresh Leaf Tissue. Phytochemical Bulletin, 19(1), 11–15. Retrieved from https://webpages.uncc.edu/~jweller2/pages/BINF8350f2011/BINF8350_Readings/Doyle_plantDNAextractCTAB_1987.pdf

Eagles, D., Walker, P.J., Zalucki, M.P., & Durr, P.A. (2013). Modelling Spatio-Temporal Patterns of Long-Distance Culicoides Dispersal into Northern Australia. Preventive Veterinary Medicine, 110(3–4), 312–322. https://doi.org/10.1016/j.prevetmed.2013.02.022

Ha, C., Coombs, S., Revill, P.A., Harding, R.M., Vu, M., & Dale, J.L. (2008). Design and Application of Two Novel Degenerate Primer Pairs for The Detection and Complete Genomic Characterization of Potyviruses. Archives of Virology, 153(1), 25–36. https://doi.org/10.1007/s00705-007-1053-7

Hondo, A., Damaiyanti, K.C., Hafizh, M.F., Amanatillah, N.E., & Damayanti, T.A. (2018). Penyakit Bercak Kuning Ubi Jalar di Bogor, Jawa Barat. Jurnal Fitopatologi Indonesia, 14(2), 69–73. https://doi.org/10.14692/jfi.14.2.69

Jones, R.A.C. (2016). Chapter Three - Future Scenarios for Plant Virus Pathogens as Climate Change Progresses. In Margaret Kielian, Karl Maramorosch, Thomas C. Mettenleiter (Eds.), Advances in Virus Research (1st ed., Vol. 95, pp. 87–147). Cambridge, MA, United States: Academic Press. https://doi.org/10.1016/bs.aivir.2016.02.004

Jones, R.A.C. & M.J. Barbetti. (2012). Influence of Climate Change on Plant Disease Infections and Epidemics Caused by Viruses and Bacteria. CAB Reviews, 7(22), 1–32. https://doi.org/10.1079/PAVSNNR20127022

Kwak, H.R., Kim, M.K., Jung, M.N., Lee, S.H., Park, J.W., Kim, K.H., ... Choi, H.S. (2007). Genetic Diversity of Sweet potato feathery mottle virus from Sweet Potatoes in Korea. The Plant Pathology Journal, 23(1), 13–21. https://doi.org/10.5423/PPJ.2007.23.1.013

Loebenstein, G., Thottappilly, G., Fuentes, S., & Cohen, J. (2009). Virus and Phytoplasma Diseases. In Loebenstein, G. & G. Thottappilly (Eds.), The Sweetpotato (pp. 105–134). Dordrecht, Netherlands: Springer. https://doi.org/10.1007/978-1-4020-9475-0

Maina, S., Coutts, B.A., Edwards, O.R., de Almeida, L., Kehoe, M.A., Ximenes, A., & Jones, R.A.C. (2017). Zucchini yellow mosaic virus Populations from East Timorese and Northern Australian Cucurbit Crops: Molecular Properties, Genetic Connectivity and Biosecurity Implications. Plant Disease, 101(7), 1236–1245. https://doi.org/10.1094/PDIS-11-16-1672-RE

Maina, S., Barbetti, M.J., Edwards, O.R., Almeida, L.D., Ximenes, A., & Jones, R.A.C. (2018). Sweet potato feathery mottle virus and Sweet potato virus C from East Timorese and Australian Sweetpotato: Biological and Molecular Properties, and Biosecurity Implications. Plant Disease, 102(3), 589–599. https://doi.org/10.1094/PDIS-08-17-1156-RE

Miano, D.W., LaBonte, D.R., Clark, C.A., Valverde, R.A., Hoy, M.W., Hurtt, S., & Li, R. (2006). First Report of a Begomovirus Infecting Sweet potato in Kenya. Plant Disease, 90(6), 832. https://doi.org/10.1094/PD-90-0832B

Maruthi, M.N., Rekha, A.R., & Muniyappa, V. (2007). Pumpkin Yellow Vein Mosaic Disease is Caused by Two Distinct Begomoviruses: Complete Viral Sequences and Comparative Transmission by an Indigenous Bemisia tabaci and The Introduced B-Biotype. Bulletin OEPP/EPPO Bulletin, 37(2), 412–419. https://doi.org/10.1111/j.1365-2338.2007.01127.x

Pusat Data dan Informasi Pertanian. (2016). Outlook Komoditas Pertanian Sub Sektor Tanaman Pangan. Jakarta, Indonesia: Kementerian Pertanian RI. Retrieved from https://epublikasi.pertanian.go.id/arsip-outlook/81-outlook-tanaman-pangan

Rannali, M., Czekaj, V., Jones, R.A.C., Fletcher, J.D., Mu, L., Davis, R., Gweyer, D.I., Coutts, B., & Valkonen, J.P.T. (2008). Molecular Genetic Characterization of Sweet potato virus G (SPVG) Isolates from The Areas of The Pacific Ocean and Southern Africa. Plant Disease, 92(9), 1313–1320. https://doi.org/10.1094/PDIS-92-9-1313

Souto, E.R., Sim, J., Chen, J., Valverde, R.A., & Clark, R.A. (2003). Properties of Strains of Sweet potato feathery mottle virus and Two Newly Recognize Potyviruses Infecting Sweet Potato in The United States. Plant Disease, 87(10), 1226–1232. https://doi.org/10.1094/PDIS.2003.87.10.1226

Valverde, R.A., Clark, C.A., & Valkonen, J.P. (2007). Viruses and Virus Disease Complexes of Sweetpotato. Plant Viruses, 1(1), 116–126. Retrieved from http://www.globalsciencebooks.info/Online/GSBOnline/images/0706/PV_1(1)/PV_1(1)116-126o.pdf

Yinghong, G., Haoru, T., & Yizheng, Z. (2006). Cloning and Sequence Analysis of Coat Protein Gene of Sweet potato virus G. Chinese Agricultural Science Bulletin, 22(9), 50–55.



DOI: https://doi.org/10.22146/jpti.64545

Article Metrics

Abstract views : 768 | views : 601

Refbacks

  • There are currently no refbacks.




Copyright (c) 2021 Jurnal Perlindungan Tanaman Indonesia

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

 height=auto

Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print)ISSN 2548-4788 (online) is published by Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.  

View website statistics