* Corresponding Author
Morphological and Molecular Identification of Colletotrichum spp. Associated with Chili Anthracnose Disease in Yogyakarta Region
https://doi.org/10.22146/jpti.58955 Dhika Sri Anggrahini(1), Arif Wibowo(2*), Siti Subandiyah(3)
(1) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281
(2) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281
(3) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281
(*) Corresponding Author
Abstract
Colletotrichum sp., the causal agent of anthracnose disease, is one of the important pathogenic fungi in chili which can cause considerable yield losses, especially during the rainy season. This study aimed to identify the species of Colletotrichum isolates obtained from chili cultivation area in The Special Region of Yogyakarta Province both morphologically and molecularly. As a comparison, a Colletotrichum isolate obtained from Magelang Regency, Central Java Province was used as comparison isolate. From the isolation result, it was obtained 14 isolates of Colletotrichum that generally had conidia that were fusiform to cylindrical with two pointed or slightly blunt ends, or crescent shapes with a various size range between 9.02-19.38 µm x 2.37–8.57 µm. Based on morphological observations using UPGMA analysis, these 14 isolates could be divided into 4 groups with 7 different types. Representative isolates of each type in different groups and a comparison isolate were identified molecularly by multi-gene analysis using the ITS1-4, gapdh and tub2 genes. The result showed that B1, G1, K2 and Mg isolates were closely related to Colletotrichum scovillei, J1 with C. truncatum; S1 and S2 with C. siamense; and J2 with C. makassarii. From the pathogenicity test on wounded chili, it showed that C. scovillei and C. siamense isolates had higher virulence than C. truncatum and C. makassarii isolates.
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Andriani, D., S. Wiyono, & Widodo. 2017. Sensitivity of Colletotrichum spp. on Chili to Benomyl, Chlorotalonil, Mancozeb, and Propineb. Jurnal Fitopatologi Indonesia 13: 119-126.
Backman, P.A., J.C. Williams, & M.A. Crawford. 1982. Yield Losses in Soybeans from Anthracnose Caused by Colletotrichum truncatum. Plant Disease 66: 1032-1034.
Caires, N.P., D.B. Pinho, J.S.C. Souza, M.A. Silva, D.O. Lisboa, O.L. Pereira, & G.Q. Furatado. 2014. First Report of Anthracnose on Pepper Fruit Caused by Colletotrichum scovillei in Brazil. Plant Disease 98: 1437. DOI: 10.1094/PDIS-04-14-0426-PDN.
Central Bureau of Statistics. 2017. Produktivitas Tanaman Sayur-sayuran Menurut Jenisnya dan Kabupaten/Kota di Provinsi Yogyakarta. https://yogyakarta.bps.go.id/, modified 20/07/2020. 1 p.
Damm, U., P.F. Cannon, J.H.C. Woudenberg, & P.W. Crous. 2012. The Colletotrichum acutatum Species Complex. Studies in Mycology73: 37-113.
Damm, U., J.H.C. Woudenberf, P.F. Cannon, & P.W. Crous. 2009. Colletotrichum species with Curved Conidia from Herbaceous Hosts. Fungal Diversity 39: 45-87.
de Silva, D.D., J.Z. Groenewald, P.W. Crous, P.K. Ades, & A. Nasruddin. 2019. Identification, Prevalence and Pathogenicity of Colletotrichum Species Causing Anthracnose of Capsicum annuum. IMA Fungus 10: 1-32.
de Silva, D.D., P.K. Ades, P.W. Crous, & P.W.J. Taylor. 2017. Colletotrichum Species Associated with Chili Anthracnose in Australia. Plant Pathology 66: 254-267.
Eryna, E.R., Wijoyo, & Widodo. 2017. Identifikasi Colletotrichum spp. Asal Pepaya. Jurnal Fitopatologi Indonesia 13: 175-183.
Guerber, J.C., B. Liu, J.C. Correll, & P.R. Johnston. 2003. Characterization of Diversity in Colletotrichum acutatum sensu lato by Sequence Analysis of Two Genes Introns, mtDNA and Intron RFLPs, and Mating Compatibility. Mycologia 95: 872-895.
Hartati, S., S. Wiyono, S.H. Hidayat, & M.S. Sinaga. 2019. Antagonism Mechanism of Epiphytic Yeast against Anthracnose Pathogen (Colletotrichum acutatum) on Chilli. Jurnal Perlindungan Tanaman Indonesia 23: 47-53.
Khalimi, K., A.A.K. Darmadi, & D.N. Suprapta. 2019. First Report of the Prevalence of Colletotrichum scovillei Associated with Anthracnose on Chili Pepper in Bali, Indonesia. International Journal of Agriculture & Biology 22: 363-368
Larkin, M.A., G. Blackshields, N.P. Brown, R. Chenna, P.A. McGettigan, H. McWilliam, F. Valentin, I.M. Wallace, A. Wilm, R. Lopez, J.D. Thompson, T.J. Gibson, & D.G. Higgins. 2007. Clustal W and Clustal X Version 2.0. Bioinformatics 23: 2947-2948.
Liu, X., B. Li, J. Cai, X. Zheng, Y. Feng, & G. Huang. 2018. Colletotrichum species Causing Anthracnose of Rubber Trees in China. Scientific Reports 8: 10435.
Liu, F., G. Tang, X. Zheng, X. Sun, X. Qi, Y. Zhou, J. Xu, H. Chen, X. Chang, Zhang S, & G. Gond. 2016. Molecular and Phenotypic Characterization of Colletotrichum Species Associated with Anthracnose Disease in Peppers from Sichuan Province, China. Scientific Reports 6: 32761.
Masanto, K. Sijam, Y. Awang, & M.G.M. Satar. 2009. The First Report of the Occurrence of Antracnose Disease Caused by Colletotrichum gloeosporioides (Penz.) Penz. & Sacc. on Dragon Fruit (Hylocereus spp.) in Peninsular Malaysia. American Journal of Applied Sciences 6: 902-912.
Meng, Y., M.L. Gleason, R. Zhang, & G. Sun. 2019. Genome Sequence Resource of the Wide-host-range Anthracnose Pathogen, Colletotrichum siamense. Molecular Plant-Microbe Interactions 32: 931-934.
Mongkolporn, O., P. Montri, T. Supakaew, & P.W.J. Taylor. 2010. Differential Reactions on Mature Green and Ripe Chili Fruit Infected by Three Colletotrichum spp. Plant Disease 94: 306-310.
National Land Board of Yogyakarta. 2013. Administration Map of Yogyakarta. Yogyakarta. http://perpustakaan.bappenas.go.id/lontar/file?file=digital/142081-[_Konten_]-DIY%20Dalam%20Angka%202014.pdf., modified 19/11/2020.
Oo, M.M., G. Lin, H.A. Jang, & S. Oh. 2017. Characterization and Pathogenicity of New Record of Anthracnose on Various Chili Varieties Caused by Colletotrichum scovillei in Korea. Mycobiology 45: 184-191.
Oo, M.M., & S.K. Oh. 2016. Chili Anthracnose (Colletotrichum spp.) Disease and its Management Approach. Korean Journal of Agricultural Science 43: 153-162.
Phoulivong, S., E.H.C. McKenzie, & K.D. Hyde. 2012. Cross Infection of Colletotrichum Species: A Case Study with Tropical Fruits. Current Research in Environmental & Applied Mycology 2: 99-111.
Prakoso, A.B., Suryanti, & A. Widiastuti. 2019. Molecular Detection of Colletotrichum spp. on Postharvest Commodities of Horticulture in Central Java and Yogyakarta, p. 020017-1–020017-6. In T.R. Nuringtyas, L. Hidayati, & M. Rafieiy (eds.), First International Conference on Bioinformatics, Biotechnology, and Biomedical Engineering (BioMIC 2018). AIP Conference Proceedings 2099, Universitas Gadjah Mada, Yogyakarta.
Prihastuti, H., L. Cai, H. Chen, E.H.C. McKenzie, & K.D. Hyde. 2009. Characterization of Colletotrichum species Associated with Coffee Berries in Northern Thailand. Fungal Diversity 39: 89–109.
Rahmat, M.S., M.S. Akhter, M.A. Maya, A.H.M.A. Rahman, & M. Akanda. 2011. Field Resistance of Chili Cultivas against Anthracnose Disease Caused by Colletotrichum capsici. Thai Journal of Agriculture Science 44: 243-250.
Ranathunge, N.P., O. Mongkolporn, R. Ford, & P.W.J. Taylor. 2012. Colletotrichum truncatum Pathosystem on Capsicum spp.: Infection, Colonization and Defense Mechanisms. Australasian Plant Pathology 41: 463-473.
Rangkuti, E.E., S. Wiyono, & Widodo. 2017. Identifikasi Colletotrichum spp. Asal Tanaman Pepaya. Jurnal Fitopatologi Indonesia 13: 175-183.
Riyandi, R. 2017. Para Petani Cabai di Sleman Alami Puso. https://www.republika.co.id., modified 12/03/2020, 1 p.
Sakthivel, K., K. Manigundan, S. Sneha, A. Patel, K. Charisma, S. Neelam, R.K. Gautam, & A. Kumar. 2018. First Report of Colletotrichum plurivorum from the Andaman and Nicobar Islands Causing Anthracnose in Chili (Capsicum annuum). New Disease Reports 38: 26.
Sato, T., J. Moriwaki, & T. Misawa. 2013. Molecular Re-identification of Strains of the Colletotrichum acutatum Species Complex Deposited in the NIAS Genebank and Morphological Characteristics of its Member Species. Japan Agricultural Research Quarterly 47: 295 – 305.
Sharma, G., & B.D. Shenoy. 2014. Colletotrichum fruticola and C. siamense are Involved in Chili Anthracnose in India. Archives of Phytopathology and Plant Protection 47: 1179-1194.
Sutardi, & C.A. Wirasti. 2017. Sistem Usahatani Cabai Merah pada Lahan Pasir di Yogyakarta. Jurnal Pengkajian dan Pengembangan Teknologi Pertanian 20: 125-139.
Sutton, B.C. 1992. The Genus Glomerella and its Anamorph Colletotrichum, p. 1-26, In J.A. Bailey & M.J. Jeger (eds), Colletotrichum: Biology, Pathology, and Control. CAB International, Wallingford.
Than, P.P., H. Prihastuti, & S. Phoulivong. 2008. Chili Anthracnose Disease Caused by Colletotrichum Species. Journal of Zhejiang University. SCIENCE. B 9: 764-778.
White, T.J., T. Bruns, S. Lee, & J. Taylor. 1990. Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics, p. 315-322. In D.H. Gelfand, M.A. Innis, J.J. Sninsky, T.J. White (eds.), PCR Protocols: A Guide to Methods and Applications. Academic Press, New York.
Wikee, S., L. Cai, N. Pairin, E.H.C. McKenzie, Y. Su, E. Chukeatirote, H.N. Thi, A.H. Bahkali, M.A. Moslem, K. Abdelsalam, & K.D. Hyde. 2011. Colletotrichum species from Jasmine (Jasminum sambac). Fungal Diversity 46: 171-182.
Woudenberg, J.H.C., M.M. Aveskamp, J. de Gruyter, A.G. Spiers, & P.W. Crous. 2009. Multiple Didymella Teleomorphs are Linked to the Phoma clematidina Morphotype. Persoonia 22: 25-62.
DOI: https://doi.org/10.22146/jpti.58955
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