* Corresponding Author
Inhibition of Rehmannia mosaic virus Infection by Ganoderma sp. Extract
https://doi.org/10.22146/jpti.49592 Mustika Ajeng Kartini Putri Pertiwi(1), Suryanti Suryanti(2*), Susamto Somowiyarjo(3)
(1) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281
(2) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281
(3) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281
(*) Corresponding Author
Abstract
In fruiting bodies and mycelia of several fungi belonging to a Basidiomycetes group, among others, Ganoderma contains active polysaccharides and has potential as an antiviral substances. This study aimed to determine the effect of mycelium and fruiting body extract of Ganoderma sp. against Rehmannia mosaic virus (ReMV) infections on Chenopodium amaranticolor with variations of dilution and application times. The results showed that mycelium and fruiting body extract of Ganoderma sp. could inhibit ReMV infections. Antiviral extract which were applied 4 hours before, and at the same time with virus inoculation resulted in a higher viral inhibition rates than when applied 4 hours after virus inoculation. Fruiting body extract at 10-1 and 10-2 dilutions delayed symptom appearance caused by ReMV infection when applied 4 hours before virus inoculation. Mycelium extract at dilution of 10-2 and 10-3 applied 4 hours before virus inoculation resulted in the highest inhibition rate (100%) which was indicated as the number of local lesions on Chenopodium amaranticolor leaves. Meanwhile the fruiting body extract dilution of 10-1 resulted in highest inhibition (99.55%) when applied 4 hours before virus inoculation.
Keywords
Full Text:
PDFReferences
Awasthi, L.P., H.N. Verma, & S. Kluge. 2016. A Possible Mechanism of Action for the Inhibition of Plant Viruses by an Antiviral Glycoprotein Isolated from Boerhaavia diffusa Roots. Journal of Virology & Antiviral Research 5: 1 – 8.
Endarsih, W., S. Hartono, & S. Sulandari. 2017. Perbaikan Metode Ekstraksi dsRNA Virus secara Sederhana untuk RT-PCR Tiga Virus Tumbuhan. Jurnal Perlindungan Tanaman Indonesia 21: 106-113.
Kobayashi, N., A. Hiramatsu, & T. Akatsuka. 1987. Purification and Chemical Properties of an Inhibitor of Plant Virus Infection from Fruiting Bodies of Lentinus edodes. Agricultural and Biological Chemistry 51: 883-890.
Kovalenko, O.G., O.M. Polishchuk, T.A. Krupodorova, N.A. Bisko, & A.S. Buchalo. 2008. Screening of Metabolites Produced by Strains of Ganoderma lucidum (Curt.:Fr.) P. Karst and Ganoderma applanatum (Pirs.:Waller) Pat. for their Activity against Tobacco mosaic virus. Visnyk Kiyvskogo Nacionalnogo Univresitrty 51: 32–34.
Kubota, K., T. Usugi, Y. Tomitaka, Y. Matsushita, M. Higashiyama, Y. Kosaka, & S. Tsuda. 2012. Characterization of Rehmannia mosaic virus Isolated from Chili Pepper (Capsicum annuum) in Japan. Journal of General Plant Pathology 78: 43 – 48.
Lu, M., Z. Han, Y. Xu, & L. Yao. 2013. In Vitro and in Vivo Anti-Tobacco mosaic virus Activities of Essential Oils and Individual Componds. Journal of Microbiology and Biotechnology 23: 771-778.
Minari, M.C., V.P. Rincao, S. Soares, & N.M.P.S. Ricardo. 2011. Antiviral Properties of Polysaccharides from Agaricus brasiliensis in the Replication of Bovine herpesvirus 1. Acta Virologica 3: 255 – 259.
Mizuno, T., G. Wang, J. Zhang, H. Kawagishi, T. Nishitoba, & J. Li. 1995. Reishi, Ganoderma lucidum and Ganoderma tsugae: Bioactive Substances and Medicinal Effects. Food Reviews International 11: 151 – 166.
Narwal, S., A. Nalasubrahmanyam, P. Sadhna, H.C. Kapoor, & M.L. Lodha. 2001. A Systemic Resistance Inducing Antiviral Protein with N-glycosidase Activity from Bougainvillea xbuttiana Leaves. Indian Journal of Experimental Biology 39: 600-603.
Nurviani. 2018. Karakterisasi Penyebab Mosaik Tembakau dan Penghambatannya dengan Ekstrak Jantung Pisang. Tesis. Program Pasca Sarjana Fakultas Pertanian Universitas Gadjah Mada, Yogyakarta. 44 p.
Purnamasari, M.I., C. Prihatna, A.W. Gunawan, & A. Suwanto. 2012. Isolasi dan Identifikasi secara Molekuler Ganoderma spp. yang Berasosiasi dengan Penyakit Busuk Pangkal Batang di Kelapa Sawit. Jurnal Fitopatologi Indonesia 8: 9-15.
Rini, K.I. 2004. Pengaruh Ekstrak Shiitake terhadap Infeksi Cucumber mosaic virus. Skripsi. Fakultas Pertanian. Universitas Gadjah Mada, Yogyakarta. 47 p.
Roostika, I., S. Hartono, & D. Efendi. 2016. Kombinasi Termoterapi dan Khemoterapi dengan Kultur Apeks dan Meristem untuk Eliminasi Virus Mosaik pada Tebu. Jurnal Litri 1: 19-28.
Rouhier, P., M. Kopp, V. Begot, M. Bruneteau, & B. Fritig. 1995. Structural Features of Fungal Beta-d-glucans for the Efficient Inhibition of the Initiation of Virus Infection on Nicotiana tabacum. Phytochemistry 1: 57 – 62.
Semangun, H. 2000. Penyakit-Penyakit Tanaman Perkebunan di Indonesia. Gadjah Mada University Press, Yogyakarta. 835 p.
Sivanandhan, S., A. Khusro, M. G. Paulraj, S. Ignacimuthu, & N.A. al-Dhabi. 2017. Biocontrol Properties of Basidiomycetes: An Overview. Journal of Fungi 1: 1-14.
Soković, M.D., J.M. Glamočlija, & A.D. Ćirić. 2013. Natural Products from Plants and Fungi as Fungicides, p. 185–232. In M. Nita (ed.), Fungicides: Showcases of Integrated Plant Disease Management from Around the World, IntechOpen Ltd., London, UK.
Somowiyarjo, S. 1999. Potensi Dua Jenis Jamur Patogen Tumbuhan, Ganoderma spp. dan Phytophthora spp., Sebagai Penghasil Senyawa Bioaktif Penghambat Sel Tumor. Mediagama 1: 47 – 51.
Tampubolon, S.D.B.M., B. Utomo, & Yunasfi. 2013. Keanekaragaman Jamur Makroskopis di Hutan Pendidikan Universitas Sumatera Utara, Desa Tongkoh Kabupaten Karo Sumatera Utara. http://download.portalgaruda.org/article.php?article=110106&val=4112, modified 11/2/2017.
Zhang, Z.C., C.Y. Lei, L.F. Zhang, X.X. Yang, R. Chen, & D.S. Zhang. 2008. The Complete Nucleotide Sequence of a Novel Tobamovirus, Rehmannia mosaic virus. Archives of Virology 153: 595 – 599.
Zhu, Q., T.H. Bang, K. Ohnuki, T. Sawai, K. Sawai, & K. Shimizu. 2015. Inhibition of Neuraminidase by Ganoderma Triterpenoids and Implications for Neuraminidase Inhibitor Design. Scientific Report 5: 1-9.
DOI: https://doi.org/10.22146/jpti.49592
Article Metrics
Abstract views : 2241
| 
views : 2483
Refbacks
- There are currently no refbacks.
Copyright (c) 2020 Jurnal Perlindungan Tanaman Indonesia
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print), ISSN 2548-4788 (online) is published by the Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
View website statistics