* Corresponding Author
Molecular Characterization of Betasatellite Associated with Begomovirus on Ageratum conyzoides in Magelang, Central Java
https://doi.org/10.22146/jpti.46579 Argawi Kandito(1), Sedyo Hartono(2*), Sri Sulandari(3), Susamto Somowiyarjo(4)
(1) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1 Bulaksumur, Sleman, Yogyakarta 55281
(2) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1 Bulaksumur, Sleman, Yogyakarta 55281
(3) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1 Bulaksumur, Sleman, Yogyakarta 55281
(4) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1 Bulaksumur, Sleman, Yogyakarta 55281
(*) Corresponding Author
Abstract
Ageratum conyzoides is one of the common weeds in Indonesia. The presence of weed can decrease plant yield by resource competition and its role as alternative hosts for pests and diseases, especially begomoviruses transmitted by whitefly. The aim of this research is to detect and characterize the begomovirus-beta satellite in A. conyzoides. A. conyzoides showed severe yellowing symptoms were collected from Magelang, Central Java, Indonesia. Total DNA was extracted and analyzed using PCR method for begomovirus and beta satellite. Begomovirus detection was performed with universal primer Krusty-Homer, resulted a 500 bp DNA fragment. Betasatelit detection performed with specific primer β01/β02, resulted a 1300 bp DNA fragment, indicated presence of a beta satellite associated with the begomovirus. The sequence of begomovirus showed 95% similarity with Tomato Leaf Curl Java Virus (ToLCJaV). The sequence of beta satellites showed 85% similarity with Tomato Leaf Curl Betasatellite (ToLCB). Characterization of beta satellite showed three main characters of beta satellite, TAATATTAC stem-loop structure, Adenine-rich region, and 118 amino acids of putative ORF BC1.
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Aji, T. M., S. Sulandari, & S. Hartono. 2015. Pengelolaan Kutu Kebul (Bemisia tabaci Gen.) dengan Sistem Barier pada Tanaman Tembakau. Jurnal Perlindungan Tanaman Indonesia 19: 6-11.
Bhattacaryya, D., P. Gnanasekaran, R. K. Kumar, N. K. Kushwaha, V. K. Sharma, M. A. Yusuf, & S. Chakraborty. 2015. A Geminivirus Betasatellite Damages the Structural and Functional Integrity of Chloroplasts Leading to Symptom Formation and Inhibition of Photosynthesis. Journal of Experimental Botany 66: 5881–5895.
Briddon, R. W. & J. Stanley. 2006. Subviral Agents Associated with Plant Single-stranded DNA Viruses. Virology 344: 198–210.
Briddon, R.W., S.E. Bull, S. Mansoor, I. Amin, & P.G. Markham. 2002. Universal Primer for PCR-mediated Amplification of DNAβ: A Molecule Associated with Some Monopartite Begomoviruses. Molecular Biotechnology. 20: 315-318.
Fontes, E.P.B., A.A. Santos, D.F. Luz, A.J. Waclawovsky, & J. Chory. 2004. The Geminivirus Nuclear Shuttle Protein is a Virulence Factor that Suppresses Transmembrane Receptor Kinase Activity. Genes & Development 18: 2545–2556.
Haselwood, E.L. & G.G. Motter. 1996. Handbook of Hawaiian Weeds. University of Hawaii Press, Honolulu. USA. 491 p.
Hidayat, S.H., E.S. Rusli, Nooraidawati. 1999. Penggunaan Primer Universal dalam Polymerase Chain Reaction untuk Mendeteksi Virus Gemini pada Cabe, hlm. 355-359 , Prosiding Seminar Ilmiah dan Kongres Nasional PFI XV. Purwokerto. 16-18 September 1999.
Hussain, M., S. Mansoor, S. Iram, A.N. Fatima, & Y. Zafar. 2005. The Nuclear Shuttle Protein of Tomato Leaf Curl New Delhi Virus is a Pathogenicity Determinant. Journal of Virology 79: 4434–4439.
Idris, A.M., M.S. Shahid, R.W. Briddon, A.J. Khan, J.K. Zhu, & J.K. Brown. 2011. An Usual Alphasatellite Associated with Monopartite Begomoviruses Attenuates Symptoms and Reduces Betasatellite Accumulation. Journal of General Virology 92: 706-717.
Kenyon, L., W.S. Tsai, S.L. Shih, & L.M. Lee. 2014. Emergence and Diversity of Begomoviruses Infecting Solanaceous Crops in East and Southeast Asia. Virus Research. 186: 104-113.
Kon, T., K. Kuwabara, S. H. Hidayat, & M. Ikegami. 2007. A Begomovirus Associated with Ageratum Yellow Vein Disease in Indonesia: Evidence for Natural Recombination between Tomato leaf curl Java virus and Ageratum yellow vein virus-[Java]. Archives of Virology 152: 1147–1157.
Kon, T., S.H. Hidayat, S. Hase, H. Takahashi, & M. Ikegami. 2006. The Natural Occurrence of Two Distinct Begomoviruses Associated with DNAβ and a Recombinant DNA in a Tomato Plant from Indonesia. Virology 96: 517-525.
Lozano, G. H. P. Trenado, E. Fiallo-Olive, D. Chirinos, F. Geraud-Pouey, R. W. Briddon, & J. Navas-Castillo. 2016. Characterization of Non-coding DNA Satellites Associated with Sweepoviruses (Genus Begomovirus, Geminiviridae) – Definition of a Distinct Class of Begomovirus-Associated Satellites. Frontiers in Microbiology 7 (162) : 1-13
Revill, P.A., C.V. Ha, S.C. Porchun, M.T. Vu, & J.L. Dale. 2003. The Complete Nucleotide Sequence of Two Distinct Begomoviruses Infecting Cucurbits in Vietnam. Archive of Virology 148: 1523-1541.
Rigden, J.E., L.R. Krake, M.A. Rezaian, & I.B. Dry. 1994. ORF C4 of Tomato leaf curl Geminivirus is a Determinant of Symptoms Severity. Virology 204: 847-850.
Saunders, K., A. Norman, S. Gucciardo, & J. Stanley. 2004. The DNA β Satellite Component Associated with Ageratum Yellow Vein Disease Encodes an Essential Pathogenicity Protein (βC1). Virology 324: 37-47.
Saunders, K., I.D. Bedford, R.W. Briddon, P.G. Markham, S.M. Wong, & J. Stanley. 2000. A Unique Virus Complex Causes Ageratum Yellow Vein Disease. PNAS 97: 6890-6895.
Shahid, M.S., M. Ikegami, A. Waheed, R.W. Briddon, & K.T. Natsuaki. 2014. Association of an Alphasatellite with Tomato yellow leaf curl virus and Ageratum yellow vein virus in Japan is Suggestive of a Recent Introduction. Viruses 6: 189-200.
Subagyo, V.N.O. & P. Hidayat. 2014. Neraca Kehidupan Kutukebul Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) pada Tanaman Cabai dan Gulma Babadotan pada Suhu 25 °C dan 29 °C. Jurnal Entomologi Indonesia 11: 11-18.
Sulandari, S. R. Suseno, S.H. Hidayat, K. Harjosudarmo, & S. Sosromarsono. 2001. Deteksi Virus Gemini pada Cabai di Daerah Istimewa Yogyakarta, Prosiding Kongres Nasional XVI dan Seminar Ilmiah PFI, Bogor, 23-24 Agustus 2001
Wang, B., F. Li, C.J. Huang, X. Yang, Y.J, Qian, Y. Xie, & X. Zhou. 2013. V2 of Tomato yellow leaf curl virus Can Suppress Methylation-mediated Transcriptional Gene Silencing in Plants. Journal of General Virology 95: 225-230.
Wilisiani, F., S. Somowiyarjo, & S. Hartono. 2014. Identifikasi Molekuler Virus Penyebab Penyakit Daun Keriting Isolat Bantul pada Melon. Jurnal Perlindungan Tanaman Indonesia 18: 47-54.
Zhou, X. 2013. Advances in Understanding Begomovirus Satellites. Annual Review Phytopathology. 51: 357–381.
DOI: https://doi.org/10.22146/jpti.46579
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