* Corresponding Author
Antagonism Mechanism of Epiphytic Yeast against Anthracnose Pathogen (Colletotrichum acutatum) on Chilli
https://doi.org/10.22146/jpti.40951 Sri Hartati(1*), Suryo Wiyono(2), Sri Hendrastuti Hidayat(3), Meity Suradji Sinaga(4)
(1) Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Padjadjaran Jln. Raya Bandung-Sumedang KM 21 Jatinangor, Sumedang, West Java 45363
(2) Departement of Plant Protection, Faculty of Agriculture, IPB University Jln. Kamper, Kampus IPB Darmaga, Bogor, West Java 16680
(3) Departement of Plant Protection, Faculty of Agriculture, IPB University Jln. Kamper, Kampus IPB Darmaga, Bogor, West Java 16680
(4) Departement of Plant Protection, Faculty of Agriculture, IPB University Jln. Kamper, Kampus IPB Darmaga, Bogor, West Java 16680
(*) Corresponding Author
Abstract
Epiphytic yeasts have the potency as antagonistic agents against various pathogens of post-harvest products. Anthracnose is a major disease of chilli that causes high economic loss. This research was objected to study the antagonism mechanism of epiphytic yeast isolates that have the antagonistic potency against anthracnose pathogen on chilli (Colletotrichum acutatum). Twenty-two isolates of epiphytic yeasts, isolated from chilli leaves and fruit, were tested. The characterization of the antagonism was carried out by antibiosis, anti-fungus volatile production, and chitinolytic activity tests. The results showed that all tested isolates did not have antibiosis mechanism against C. acutatum. All isolates produced volatile compounds which inhibited the colony growth of C. acutatum. Four isolates showed high relative inhibition rate, i.e. isolates B32DEP (35.68%), B30DEP (37.52%), B23DEP (38.52%), and B29DEP (45.42%). Fourteen isolates showed chitinolytic activities. Three of them had high chitinolytic activities, i.e. B12DEP, B2DEP, and G237DEP.
Keywords
Full Text:
PDFReferences
Agrios, G.N. 2005. Plant Pathology. 4th ed. Acad. Press, New York. 922 p.
Avis, T. J., & R.R. Bélanger. 2002. Mechanisms and Means of Detection of Biocontrol Activity of Pseudozyma Yeasts against Plant-Pathogenic Fungi. FEMS Yeast Research 2: 5−8.
AVRDC (Asian Vegetable Research Development and Center). 2004. Evaluation of Phenotypic and Molecular Criteria for the Identification of Colletotrichum Species Causing Pepper Anthracnose in Taiwan, p. 92−93. In Warwick Easdown & Thomas Kalb (ed.), AVRDC Report. AVRDC, Shanhua, Taiwan.
Carstens, M., M.A. Vivier, P. Van Rensburg, & I.S. Pretorius. 2003. Overexpression, Secretion and Antifungal Activity of the Saccharomyces cerevisiae Chitinase. Annals of Microbiology 53:1 5−28.
Castoria, R., F. De Curtis, G. Lima, L. Caputo, S. Pacifico, & V. De Cicco. 2001. Aureobasidium pullulans (LS-30) an Antagonist of Postharvest Pathogens of Fruits: Study on its Modes of Action. Postharvest Biology Technology 22: 7−17.
Chanchaichaovivat, A., P. Ruenwongsa, & B. Panijpan. 2007. Screening and Identification of Yeast Strains from Fruits and Vegetables: Potential for Biological Control of Postharvest Chilli Anthracnose (Colletotrichum capsici). Biological Control 42: 326−335.
Droby, S., R. Hofstein, C.L. Wilson, M. Wisniewski, B. Fridlender, L. Cohen, B. Weiss, A. Daus, D. Timar, & E. Chalutz. 1993. Pilot Testing of Pichia guilliermondii: A Biocontrol Agent for Postharvest Diseases of Citrus Fruit. Biological Control 3: 47–52.
Francesco, A.D., L. Ugolini, L. Lazzeri, & M. Mari. 2014. Production of Volatile Organic Compounds by Aureobasidium pullulans as a Potential Mechanism of Action against Postharvest Fruit Pathogens. Biological Control 81: 8−14.
Hartati, S. 2016. Khamir sebagai Agens Biokontrol Antraknosa (Colletotrichum acutatum) pada Tanaman Cabai Pascapanen. Disertasi. Institut Pertanian Bogor, Bogor. 94 p.
Hsu, S.C., & J. L. Lockwood. 1975. Powdered Chitin Agar as a Selective Medium for Enumeration of Actinomycetes in Water and Soil. Applied Microbiology 29: 422–426.
Huang, R., G.Q. Li, J. Zhang, L. Yang, H.J. Che, D.H. Jiang, & H.C. Huang. 2011. Disease Control and Pest Management Control of Postharvest Botrytis Fruit Rot of Strawberry by Volatile Organic Compounds of Candida intermedia. Phytopathology 101: 859−869.
Irtwange, S.V. 2006. Application of Biological Control Agents in Pre- and Postharvest Operations. Agricultural Engineering International the CIGR Ejournal 3: 1−12.
Liu, P., L. Luo, & C. Long. 2013. Characterization of Competition for Nutrients in the Biocontrol of Penicillium italicum by Kloeckera apiculata. Biological Control 67: 157−162.
Morath, S.U., R. Hung, & J.W. Bennett. 2012. Review Fungal Volatile Organic Compounds: A Review with Emphasis on their Biotechnological Potential. Fungal Biology Reviews 26: 73−83.
Nunes, C.A. 2012. Biological Control of Postharvest Diseases of Fruit. European Journal Plant Pathology 133: 181−196.
Qing. F., & T. Shiping. 2000. Postharvest Biological Control of Rhizopus Rot of Nectarine Fruits by Pichia membranefaciens. Plant Disease 84: 1212−1216.
Sangdee, A., S. Sachan, & S. Khankhum. 2011. Morphological, Pathological and Molecular Variability of Colletotrichum capsici Causing Anthracnose of Chilli in the North-East of Thailand. African Journal of Microbiology Research 5: 4368−4372.
Sharma, R.R., D. Singh, & R. Singh. 2009. Biological Control of Postharvest Diseases of Fruits and Vegetables by Microbial Antagonists: A Review. Biological Control 50: 205–221.
Shurtleff, M.C., & C.W. Averre. 1997. The Plant Disease Clinic and Field Diagnosis of Abiotic Diseases. APS Press, St Paul, Minnesota, USA. 242 p.
Sugiprihatini, D., S. Wiyono, & Widodo. 2011. Selection of Yeasts Antagonists as Biocontrol Agent of Mango Fruit Rot caused by Botryodiplodia theobromae. Microbiology Indonesia 5:154−159.
Than, P.P., H. Prihastuti, S. Phoulivong, P.W.J. Taylor, & K.D. Hyde. 2008. Review: Chilli Anthracnose Disease Caused by Colletotrichum Species. Journal of Zheijang University SCIENCE B 9: 764−778.
Walker, G.M., A.H. Mcleod, & H.J. Hodgson. 1995. Interactions between Killer Yeasts and Pathogenic Fungi. FEMS Microbiology Letters 127: 213−222.
Wardhika, C.M., Suryanti, & T. Joko. 2014. Eksplorasi Bakteri yang Berpotensi sebagai Agens Pengendali Hayati Fusarium solani dan Meloidogyne incognita pada Lada. Jurnal Perlindungan Tanaman Indonesia 18: 89–94.
Yanai, K., N. Takaya, N. Kojima, H. Horiuchi, A. Ohta, & M. Takagi. 1992. Purification of Two Chitinases from Rhizopus oligosporus and Isolation and Sequensing of the Encoding Genes. Journal of Bacteriology 57: 7398−7406.
Zhang RL, Huang JS. 2007. Cloning of a Carbendazim-resistant Gene from Colletotrichum gloeosporioides of Mango in South China. African Journal of Biotechnology 6:143−147.
Zhang, H., L. Wang, Y. Dong, S. Jiang, J. Cao, & R. Meng. 2007. Postharvest Biological Control of Gray Mold Decay of Strawberry with Rhodotorula glutinis. Biological Control 40: 287−292.
DOI: https://doi.org/10.22146/jpti.40951
Article Metrics
Abstract views : 3202
| 
views : 2443
Refbacks
- There are currently no refbacks.
Copyright (c) 2019 Jurnal Perlindungan Tanaman Indonesia
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print), ISSN 2548-4788 (online) is published by the Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
View website statistics