The Effect of Silica (SiO2) to the Severity of Yellow Leaf Curl Disease on Chili Pepper

https://doi.org/10.22146/jpti.38951

Dewa Gede Wiryangga Selangga(1), Sri Hendrastuti Hidayat(2*), Anas Dinurrohman Susila(3), Suryo Wiyono(4)

(1) Department of Plant Protection, Faculty of Agriculture, IPB University Jln. Kamper, Kampus IPB Dramaga, Bogor, West Java 16680
(2) Department of Plant Protection, Faculty of Agriculture, IPB University Jln. Kamper, Kampus IPB Dramaga, Bogor, West Java 16680
(3) Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University Jln. Meranti, Kampus IPB Dramaga, Bogor, West Java 16680
(4) Department of Plant Protection, Faculty of Agriculture, IPB University Jln. Kamper, Kampus IPB Dramaga, Bogor, West Java 16680
(*) Corresponding Author

Abstract


Yellow leaf curl disease of chili pepper caused by Pepper yellow leaf curl virus (PYLCV) has been reported as an important disease in Java and Bali. Disease severity reached 80−100% and it may cause significant yield losses. In order to reduce the negative impact of synthetic insecticides, silica application was evaluated for its potency to suppress the disease. A greenhouse experiment was conducted using randomly block design with 2 factors: PYLCV isolate (Java and Bali) and silica (SiO2) treatment (with and without). Parameters observed were disease symptoms, incubation period, disease incidence and severity, and total silica level. The symptoms of virus infection in Pelita 8 and Seret cultivars were yellow mosaic, leaf curl, green mosaic, dwarf, and cupping upward or downward. A Specific DNA fragment of 912 bp was successfully amplified from 4 samples. Four sequences were obtained and further analysis showed their highest homology, i.e. 96% and 97% with Pepper yellow leaf curl Indonesia virus-Java (PYLCIV-Java) (JX416180) and PYLCIV-KrthAl (LC381274), respectively. Infection by different virus isolates did not affect disease severity significantly. The application of silica was able to delay symptom development and to suppress the severity of the disease in the range of 16.67−30.33%. Silica application on the soil increased the total content of silica in the plants. However, a further experiment is required to understand the mode of action of silica in inducing plant resistance to the pathogen.


Keywords


disease severity; incubation period; insect vector; pepper yellow leaf curl virus; sequence homology

Full Text:

PDF


References

Adilah, N.F. & S.H. Hidayat. 2014. Intensity of Yellow Leaf Curl Disease and Population Growth of Whitefly on Chili Pepper Genotypes. Jurnal Fitopatologi Indonesia 10:198.

Almeida, G.D., D. Pratissoli, J.C. Zanuncio, V.B. Vicentini, A.M. Holtz, & J.C. Serrao. 2008. Calcium Silicate and Organic Mineral Fertilizer Applications Reduce Phytophagy by Thrips palmi   Karny (Thysanoptera: Thripidae) on Eggplants (Solanum melongena L.). Interciencia 33: 835–838.

Almeida, G.D., D. Pratissoli, J.C. Zanuncio, V.B. Vicentini, A.M. Holtz, & J.C. Serrao. 2009. Calcium Silicate and Organic Mineral Fertilizer Increase the Resistance of Tomato Plants to Frankliniella schultzei. Phytoparasitica 37: 225–230.

Cacique, I.S., G.P. Domiciano, W.R. Moreira, F.A. Rodrigues, M.F.A. Cuz, N.S. Serra, & A.B. Catalia. 2013. Effect of Root and Leaf Applications of Solube Silicon on Blast Development in Rice. Bragantia Campinas 72: 304−309.

Correa, R.S.B., J.C. Moraes, A.M. Auad, & G.A. Carvalho.  2005.  Silicon  and Acibenzolar-s- Methyl as Resistance Inducers in Cucumber, against the Whitefly Bemisia tabaci (Genna- dius) (Hemiptera: Aleyrodidae) Biotype B. Neotropical Entomology 34: 429–433.

Czosnek, H., A. Hariton-Shalve, I. Sobol, R. Gorovits, & M. Ghanim. 2017. The Incredible Journey of Begomovirus in their Whitefly Vector. Viruses 9: 273.

Doyle, J.J. & J.L. Doyle. 1999. Isolation of Plant DNA from Fresh Tissue. Focus 12: 13−15.

Faizah, R., S. Sujiprihati, M. Syukur, & S.H. Hidayat. 2012. Ketahanan Biokimia Tanaman Cabai terhadap Begomovirus Penyebab Daun Keriting Kuning. Jurnal Fitopatologi Indonesia 8: 138−144.

Friarini, Y.P., Witjaksono, & Suputa. 2016. Study of the Use of Maize as Barrier Crop in Chili to Control Bemisia tabaci (Gennadius) Population. Jurnal Perlindungan Tanaman Indonesia 20:79−83.

Gomes, F.B., J.C. Moraes, C.D. Santos, & M.M. Goussain. 2005. Resistance Induction in Wheat Plants by Silicon and Aphids. Scientia Agricola 62: 547–551.

Heine, G., G. Tikum, & W.J. Horst. 2007. The Effect os Silicon on the Infection by and Spread of Pythium aphanidermatum in Single Roots of Tomato and Bitter Gourd. Journal of Experimental Botany 58: 569−577.

Inbar, M., H. Doostdar, D. Gerling, & R.T. Mayer. 2001. Induction of Systemic Acquired Resistance in Cotton by BTH has a Negligible Effect on Phytophagous Insects. Entomologia Experimentalis et Applicata 99: 65–70.

Krishardianto, A. & D. Sukma. 2017. Morphological Characterization and Effects of Treatments Fertilization and Gift Sillica (Si) on Genotype Hybrid Catteleya Orchids. BuletinAgrohorti 5: 167−175.

Lestari, S.M., S.H. Hidayat, & Widodo. 2018. Determination of Endophytic Fungi as Induce Resistance Agent if Chili Pepper against Pepper Yellow Leaf Curl Disease. Agrivita 40: 249−256.

Li, R., S. Salih, & S. Hurtt. 2004. Detection of Geminiviruses in Sweetpotato by Polymerase Chain Reaction. Plant Disease. 88: 1347–1351.

Ma, J.F. & N. Yamaji. 2008. Functions and Transport of Silicon in Plants. Cellular and Molecular Life Sciences 65: 3049–3057.

Makarim, A.K., E. Suhartatik, & Kartohardjono. 2007. Silikon: Hara Penting pada Sistem Produksi Padi. Iptek Tanaman Pangan 2: 196.

NCBI (National Center for Biotechnology Information). 2018. https://blast.ncbi.nlm.nih. gov/Blast.cgi. modified 12/01/18.

Norris, R.F., E.P. Caswell-Chen, & M. Kogan. 2003. Concepts in Integrated Pest Management. Prentice Hall, Upper Saddle River, New Jersey. 586 p.

Priwiratama, H., S.H. Hidayat, & Widodo. 2012. Pengaruh Empat Galur Bakteri Pemacu Per- tumbuhan Tanaman dan Waktu Inokulasi Virus terhadap Keparahan Penyakit Daun Keriting Kuning Cabai. Jurnal Fitopatolologi Indonesia 8: 1−8.

Putra, I.G.N.B.P., N.M. Puspawati, I.D.N. Nyana, I.K. Siadi, & G. Suastika. 2015. Identifikasi Virus yang Berasosiasi dengan Penyakit Mosaik, Kuning, dan Klorosis pada Tanaman Cabai Rawit (Capsicum Frutescens L.). E-Jurnal Agroekoteknologi Tropika 4: 251.

Roesmarkam & N.W. Yuwono. 2002. Ilmu Kesuburan Tanah. Kanisius, Yogyakarta. 225 p.

Rojas, M.R., R.L. Gilbertson, D.R. Russel, & D.P. Maxwell. 1993. Use of Degenerate Primers in the Polymerase Chain Reaction to Detect Whitefly Transmitted Geminiviruses. Plant Disease 77: 340−347.

Sapei, L., K.S. Padmawijaya, A. Sutejo, & L. Theresia. 2015. Karakterisasi Silika Sekam Padi dengan Variasi Temperatur Leaching Meng- gunakan Asam Asetat. Jurnal Teknik Kimia 19: 38−43.

Setiawati, W., B.K. Udiarto, & T.A. Soetiarso. 2008. Pengaruh Varietas dan Sistem Tanam Cabai Merah terhadap Penekanan Populasi Hama Kutu Kebul. Journal of Horticulture 18: 55−61.

Song, F. & S.M. Swinton. 2009. Returns to Integrated Pest Management Research and Outreach for Soybean Aphid. Journal of Economic Entomology 102: 2116−2125.

Sulandari, S., R. Susesno, S.H. Hidayat, J. Harjosudarmo, & S. Sosromarsono. 2006. Deteksi dan Kajian Kisaran Inang Virus Penyebab Penyakit Daun Keriting Kuning Cabai. Hayati 13: 1−6.

Wattanapayakul, W., A. Polthanee, B. Siri, N.N. Bhadalung, & A. Promkhambut. 2011. Effects of Silicon  in  Suppressin g  Blast  Disease  and Increasing Grain Yield of Organic. Rice in Northeast Thailand. Asian Journal of Plant Pathology 5: 134−145.

Yasa, I.N.D., I.P. Sudiarta, I.G.N.A.S. Wirya, K. Sumiartha, I.M.S. Utama, G.C. Luther, & J. Mariyono. 2012. Kajian Ketahanan terhadap Penyakit Busuk Daun (Phytophthora infestans) pada Beberapa Galur Tomat. E-Jurnal Agroekoteknologi Tropika 1: 154−161.



DOI: https://doi.org/10.22146/jpti.38951

Article Metrics

Abstract views : 294 | views : 109

Refbacks

  • There are currently no refbacks.




Copyright (c) 2019 Jurnal Perlindungan Tanaman Indonesia

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.


Accredited Journal, Based on Decree of the Minister of Research, Technology and Higher Education, Republic of Indonesia Number 30/E/KPT/2018, Vol. 21 No. 1 the Year 2017 - Vol. 25 No. 1 the Year 2021. Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print), ISSN 2548-4788 (online) is indexed by: :