The Role of Anthocyanin Content of Garden Balsam’s (Impatiens balsamina L.) Flower Extract on the Growth of Ralstonia solanacearum

Djati Widhityarini(1*), Rani Agustina Wulandari(2), Triwidodo Arwiyanto(3)

(1) Graduate Program of Plant Breeding Science, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1 Bulaksumur, Sleman, Yogyakarta 55281
(2) Department of Agronomy, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1 Bulaksumur, Sleman, Yogyakarta 55281
(3) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1 Bulaksumur, Sleman, Yogyakarta 55281
(*) Corresponding Author


The study was aimed to determine the response of bacterial growth of Ralstonia solanacearum  Race  I of  biovar  III  phylotype  I  treated  with  flower  extract  of Garden Balsam (Impatiens balsamina L.) on different color of flower, that was able to give the strongest antibacterial compound and to determine the physical characteristics of I. balsamina in order to facilitate the purpose of the garden balsam cultivation as antibacterial. In addition, this study also aimed to determine the highest total anthocyanin content (TAC) and to determine the molecular characteristics of anthocyanin-coding genes and DNA base sequences of I. balsamina that indicated antibacterial properties. The research used Completely Randomized Design (CRD) 2 X 4 each with 3 replications. The first factor was the method of plating bacteria culture (pour plate and streak plate method), the second factor was the color of the garden balsam flower extracts consisted of white, red, purple and magenta. Therefore, the experiment consisted of 8 treatments of combination. Data were analyzed using CRD (α = 0.05). The results showed that the average combination of pour plate and red flower extract gave the value of resistance velocity on the 3rd day with the diameter of the inhibition zone was 33.46 mm, with the inhibitory zone diameter of the garden balsam extract of 22.90 mm. Red flower plants showed a bright red stem on the braches and the pointed leaf edge. Although this red does not show the total content of anthocyanin compounds higher than purple, however from RAPD analysis for red color indicated the anthocyanin of pelargonidin as an anthocyanin compound responsible for its antibacterial properties.


antibacterial; anthocyanin; flower color; Impatiens balsamina; Ralstonia solanacearum

Full Text:



Adfa, M. 2007. Senyawa Antibakteri dari Daun Pacar Air (Impatiens balsamina Linn.) Gradien 4: 318-322.

Amrullah, A. W. & Y. Ratna. 2015. Aktivitas Antibakteri Ekstrak Etanol Daun Pacar Air (Impatiens balsamina L.) terhadap Bakteri Escherichia coli Multiresisten dan Staphylococcus aureus Multiresisten serta Bioautografinya. Skripsi. Fakultas Farmasi, Universitas Muhammadiyah Surakarta, Surakarta. 16 p.

Arwiyanto, T. 2014. Ralstonia solanacearum. Biologi, Penyakit yang Ditimbulkan dan Pengelolaannya. Gadjah Mada University Press, Yogyakarta. 111 p.

Dalimartha, S. 2003. Atlas Tumbuhan Obat Indonesia Jilid 3. Trubus Agriwidya. Jakarta. 198 p.

Doyle, J. J. & J. L. Doyle. 1990. Isolation of Plant DNA from Fresh Tissue. Focus 12: 13–15.

Fatmawati, Y. 2016. Keragaman Morfologi dan Molekuler Empat Kelompok Kultivar Jagung (Zea mays L.). Skripsi. Fakultas Pertanian Universitas Gadjah Mada. Yogyakarta. 101 p.

Giusti, M.Monica & R.E. Wrolstad. 2001. Characteristic and Measurement of Anthocyanins by UV-Visible Spectroscopy, Current Protocols in Food Analytical Chemistry, John Wiley & Sons Inc., F1.2.1-F1.2.13. 13 p.

Ishiguro, K., H. Fukumoto, T. Murashima, M. Kuriyama, M. Semma, & K. Isoi. 2015. Antianaphylactic Effects of the Ethanolic Extract from the Petals of Impatiens balsamina L. in Mice. Phytotherapy Research 11: 343-347.

Kusuma, G.A., S.N.J. Longdong, & R.A. Tumbol. 2014. Uji Daya Hambat dari Ekstrak Tanaman Pacar Air (Impatiens balsamica L.) terhadap Pertumbuhan Bakteri Aeromonas hydrophila. Akulturasi (Jurnal Ilmiah Agrobisnis Perikanan) 2: April 2014.

The Royal Horticultural Society. 2001. Britain’s Gardening Charity is Dedicated to Promoting Horticultural Excellence through its Shows, Gardens and Horticultural Expertise. RHS Large Colour Chart (Sixth Revised Edition)., modified 08/12/2016.

To, K.Y. & C.K. Wang. 2006. Floriculture, Ornamental and Plant Biotechnology Volume I: Molecular Breeding of Flower Color. Global Science Books, UK. 646 p.

Trianom, B., T. Arwiyanto, & T. Joko. 2018. Perancangan Primer Spesifik Subspesies Berbasis Gen Endoglukanase untuk Deteksi Ralstonia syzygii subsp. syzygii. Jurnal Perlindungan Tanaman Indonesia 22: 124-131.

Vankar, P. S. & J. Srivastava, 2010. Evaluation of Anthocyanin Content in Red and Blue Flowers. International Journal of Food Engineering 6: Article 7.


Article Metrics

Abstract views : 1801 | views : 1296


  • There are currently no refbacks.

Copyright (c) 2019 Jurnal Perlindungan Tanaman Indonesia

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print)ISSN 2548-4788 (online) is published by the Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.  

View website statistics