Identification of Termites’s Hindgut Bacterial Symbionts (Coptotermes curvignathus Holmgren) and the Feasibility in Rice Straw Decomposting

https://doi.org/10.22146/jpti.33912

Marheni Sembiring(1*), Yunda Gusriani(2)

(1) Department of Pests and Plants Disease, Faculty of Agriculture, Universitas Sumatera Utara Jln. Prof. Ahmad Sofian No. 3, Kampus USU, Medan, 20155
(2) Graduate Student, Faculty of Agriculture, Universitas Sumatera Utara Jln. Prof. Ahmad Sofian No. 3, Kampus USU, Medan, 20155
(*) Corresponding Author

Abstract


Cellulotic bacteria can be used as breakers of cellulose bonds in rice straw, one of which is cellulotic symbiont bacteria found in the hindgut part of termite Coptotermes curvignathus Holmgren. The study aimed to characterize and identify bacterial isolates from the back intestine of C. curvignathus termites and test the ability of bacterial isolates to decomposition rice straw. The results of isolation on worker caste termites found one bacterial species that was successfully cultured in Carboxylmethil Cellulose (CMC) media. Biochemical test results for isolates, showed that, bacteria found in rod form (rod shape), aerobic, gram negative, motile and produced catalase enzyme. Sequencing of 16S rRNA genes in bacterial isolates showed similarities with Bacillus cereus. A dose of 10 ml symbiont starter, 50% in concentration, applied to 2 kg rice straw can shorten the decomposition time from 12 weeks to 4 weeks, reducing the required decomposition time which is about 75% of the normal time.


Keywords


decomposition of rice straw; symbiotic bacteria; symbiotic starter bacteria termite hindgut

Full Text:

PDF


References

Aini, A.N. 2015. Peranan Bakteri dalam Usus Rayap Tanah Macrotermes gilvus Hagen sebagai Agen Biologis Degradasi Bahan Organik. Skripsi. Biologi. Fakultas MIPA. Universitas Negeri Semarang, Semarang. 62 p.

Ardani, F. 2016. Isolasi Bakteri Selulolitik dan Karakterisasi Enzim Selulase dari Feses Kelelawar. Tesis. Sekolah Pascasarjana. Institut Pertanian Bogor, Bogor. 31 p.

Ashwani, K., L. Saida, & K.V. Reddy. 2014. Isolation, Screening, and Characterization of Cellulolytic Bacteria from Forest Soil Sample. International Journal of Current Microbiology and Applied Sciences 3: 679−685.

Buchanan, R.E. & N.E. Gibbons. 2003. Bergey’s Manual of Determinative Bacteriology. The William & Wilkins Company, Baltimore, USA. 787 p.

Clous, D. & R.C.W. Berkeley. 1986. Genus Bacillus, p. 1105−1139. In P.H.A Sneath (ed.). Bergeys Manual of Systematic Bacteriology. Vol 2. Williams and Wilkins, Baltimore.

Diba, F., S. Khotimah, & U. Febriyana. 2012. Isolation and Identification Cellulolytic Bacteria from the Termite Coptotermes curvignatus Holmgren and Macrotermes gilvus Hagen from Secondary Forest in West Kalimantan Indonesia, p. 105−111. In P.R. Termite Research Group (eds), Proceedings of the 9th Pacific Rim Termite Research Group Conference. Hanoi. February 27−28, 2012.

Fox, G.E., K.R. Pechan, & C.R. Woese. 1977. Comparative Cataloging of 16S Ribosomal Ribonucleic Acid: Molecular Approach to Procaryotic Systematic. Journal of Systemic Bacteriology 27: 44−57.

Howard, R., L.E. Abotsi., J.V. Rensburg, & S. Howard. 2003. Lignocellulose Biotechnology: Issue of Bioconversion and Enzyme Production. Journal African of Biotechnology 2: 602−619.

Kuhnigk, T., E. Borst., A. Ritter, P. Kampfer, A. Graf, H. Hertel, & H. Konig. 1994. Degradation of Lignin Monomers by the Hindgut Flora of Xylophagous Termites. Journal Systemic and Applied Microbiology 17: 76–85.

Murbandono, L. 2000. Membuat Kompos. Edisi Revisi. Penebar Swadaya. Jakarta. 60 p.

Nandi, N., F.H. Rahman., N.B. Sinha, & J.N. Hajra. 2000. Compatibility of Lignin-Degrading and Cellulose Decomposing Fungi During Decomposition of Rice Straw. Journal of the Indian Society of Soil Science 48: 387−389.

Ni, J. & G. Tokuda. 2013. Lignocellulose-degrading Enzymes from Termites and their Symbiotic Microbiota. Journal Biotechnology Advances 31: 838–850.

Papoola, K.O.K. & A.V. Opayele. 2012. Morphometrics of Macrotermes bellicosus (African mound termite) (Blattodea: Termitidae) and the Impact of its Saliva Amylase on the Strength of Termitarium Soil. New York Science Journal 5: 207−216.

Ramin, M., A. Ralimon., N. Abdullah., J.M. Panandam, & K. Sijam. 2008. Isolation and Identification of Three Species of Bacteria from the Termite Coptotermes curvignathus (Holmgren). Present in the Vicinity of University Putra Malaysia. Journal Microbiology 3: 288–292.

Ramin, M., A.R. Alimon, & N. Abdullah. 2009. Identification of Cellulolytic Bacteria Isolated from the Termite Coptotermes curvignathus (Holmgren). Journal of Rapid Methods & Automation in Microbiology 17: 103−116.

Sutanto, R. 2002. Pertanian Organik Menuju Pertanian Alternatif dan Berkelanjutan. Penerbit Kanisius, Yogyakarta. 50 p.

Simanungkalit, R.D., M.D.A Suriadikarta., R. Sarswati., D. Setyorini, & W. Hartatik. 2012. Pupuk Organik dan Pupuk Hayati. Balai Besar Penelitian dan Pengembangan Sumber Daya Lahan Pertanian, Bogor. 283 p.

Singhania. 2009. Cellulolytic Enzymes, p. 371−381. In P. Singh., Nigam., A Pandey (eds.), Biotechnology for Agro-Industrial Residues Utilization. Springer, Dordrecht.

Subekti, N., D.Nandika., D. Duryadi., S. Suryokusumo, & S. Anwar. 2008. Sebaran dan Karakter Morfologi Rayap Tanah Macrotermes gilvus Hagen di Habitat Hutan Alam. Jurnal Ilmu dan Teknologi Hasil Hutan 1: 27−33.

Thayer, D.W. 1976. Facultative Wood-digesting Bacteria from the Hindgut of the Termite Reticulitermes hesperus. Journal of General Microbiology 95: 287−296.



DOI: https://doi.org/10.22146/jpti.33912

Article Metrics

Abstract views : 190 | views : 85

Refbacks

  • There are currently no refbacks.




Copyright (c) 2019 Jurnal Perlindungan Tanaman Indonesia

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.


Accredited Journal, Based on Decree of the Minister of Research, Technology and Higher Education, Republic of Indonesia Number 30/E/KPT/2018, Vol. 21 No. 1 the Year 2017 - Vol. 25 No. 1 the Year 2021. Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print), ISSN 2548-4788 (online) is indexed by: :