Efficacy of Biopesticide Be-Bas against Sweet Potato Weevils (Cylas formicarius Fabricius) in Tidal Land


Yusmani Prayogo(1), Marida Santi Yudha Ika Bayu(2*)

(1) Indonesia Legumes and Tuber Crops Research Institute (ILETRI) Jln. Raya Kendalpayak, KM 08, PO. BOX 66 Malang, East Java 65101
(2) Indonesia Legumes and Tuber Crops Research Institute (ILETRI) Jln. Raya Kendalpayak, KM 08, PO. BOX 66 Malang, East Java 65101
(*) Corresponding Author


In Indonesia tidal land is very wide and quite potential for sweet potatoes, but an obstacle for this cultivation is sweet potato weevil (Cylas formicarius) causes loss of 100%. This research aimed to evaluate the efficacy of biopesticide Be-Bas contains Beauveria bassiana entomopathogenic conidia to C. formicarius mortality at tidal lands. This study was conducted on June-November 2016 in South Kalimantan. The research consisted of five treatments and five replications. Results showed that the application of biopesticide Be-Bas in the planting hole and stem base was effective to cause mortality of 17 and 15 larvae per tuber, respectively. However, the application by soaking cutting was able to kill 3 larvae per tuber. The application of biopesticide Be-Bas in the planting hole and stem base were also effective to suppress the number of eggs laid by C. formicarius in the tuber, hence there was no damage found on the tuber. Meanwhile, the application of conventional insecticide was less effective because a total of 17 eggs and 3 larvae of C. formicarius were still found in each tuber. It caused tuber damage up to 17%, thus the tuber cannot be consumed. Therefore, the application of biopesticide Be-Bas in the planting hole or stem base was considered capable to suppress tubers damage caused by C. formicarius better than that of conventional insecticide.


application method; biological control; Beauveria bassiana; Cylas formicarius; entomopathogenic fungi

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Akmal, M., S. Freed, M.N. Malik, & H.T. Gul. 2013. Efficacy of Beauveria bassiana (Deuteromycotina: Hyphomycetes) against Different Aphid Species under Laboratory Conditions. Pakistan Journal of Zoology 45: 71−78.

Ali, A., H. Sermann, S. Lerche, & C. Buttner. 2009. Soil Application of Beauveria bassiana to Control Ceratitis capitata in Semi Field Conditions. Communication in Agricultural and Applied Biological Science 74: 357−361.

Alwi, M. 2014. Prospek Lahan Rawa Pasang Surut untuk Tanaman Padi, p. 45−59. In M. Yasin (ed.), Prosiding Seminar Nasional “Inovasi Teknologi Pertanian Spesifik Lokasi”. Banjarbaru, Agustus 6−7, 2014.

Ansari, M.A. & T.M. Butt. 2012. Susceptibility of Different Developmental Stages of Large Pine Weevil Hylobius abietis (Coleoptera: Curculionidae) to Entomopathogenic Fungi and Effect of Fungal Infection to Adult Weevils by Formulation and Application Methods. Journal of Invertebrate Pathology 111: 33−40.

Boyle, D. & G.C. Cutler. 2012. Effects of Insect Activity, Soil, and Cuticular Factors on Virulence of Beauveria bassiana toward Blissus leucopterus hirtus. Journal of Pest Science 85: 505−512.

Brownbridge, M., S.D. Reay, T.I. Nelson, & T.R. Glare. 2012. Persistance of Beauveria bassiana (Ascomycota: Hypocreales) as an Endophyte Following Inoculation of Radiate Pine Seed and Seedlings. Biological Control 61: 194−200.

Conceschi, M.R., G.P.D. Alessandro, Rde.A. Moral, C.G.B. Demetria, & I.D. Junior. 2016. Trans- mission Potential Entomopathogenic Fungi Isaria fumosoroseus and Beauveria bassiana from Sporulated Cadavers of Diaphorina citri and Toxoptera citricida to Uninfected D. citri Adults. Biological Control 24: 1−11.

Devi, M., K.I. Kumar, & R.F. Niranjana. 2014. Biology of Sweet Potato Weevil Cylas formicarius on Sweet Potato. Journal of Entomological Research Society 38: 53−57.

Du, W.J., P. Jiang, T. Yang, Y.Q. Bu, C.H. Liu, & C.C. Dai. 2013. Effects of Beauveria bassiana and Acephate on Enzyme Activities and Microbial in Paddy Soil.  Plant Soil and Environment 59: 562−567.

Eler, F. & A.O. Ates. 2015. Potential of Two Ento- mopathogenic Fungi, Beauveria bassiana and Metarhizium anisopliae (Coleoptera: Scarabidae), as Biological Control Agents against the June Beetle. Journal of Insect Science 15: 1−6.

Galib, R. 2014. Pengembangan Komoditas Ubijalar di Lahan Rawa Lebak Kalimantan Selatan sebagai Sumber Pangan (Kasus Di Kecamatan Daha Utara), p. 467−472. In M. Yasin (ed.), Prosiding Seminar Nasional “Inovasi Teknologi Pertanian Spesifik Lokasi”, Banjarbaru, Agustus 6−7, 2014.

Garcia-Munguia, A.M., J.A. Garza-Hernandez, E.A. Rebollar-Tellez, M.A. Rodriguez-Perez, & F. Reyes-Villaneva. 2011. Transmission of Beauveria bassiana Male to Female Aedes aegypti Mosquitoes. Parasites and Vectors 4: 1−6.

Greenfield, M., M.I. Gomez-Jimenez, & S. Parsa. 2016. Beauveria bassiana and Metarhizium anisopliae Endophytically Colonize Cassava Roots Following Soil Drench Inoculation. Biological Control 95: 40−49.

Hernandez, M.M., E.M. Villar, C. Peau, I. Perez-Morene, & V. Marco. 2012. Compatibility of the Entomopathogenic Fungus Beauveria bassiana with Flufenoxuron and Azadirahtin against Tetranychus urticae. Experimental Applied Acarology 23: 71−80.

Hue, S.H. & M.Y. Low. 2015. An Insight Into Sweet Potato Weevils Management: A Review. Psyche: A Journal of Entomology 1: 1−12.

Jadhav, R.S. & S.D. Patil. 2016. Effect of Various Adjuvants on Growth and Development of Entomopathogenic Fungi Beauveria bassiana (Balsamo) Vuillemin. International Journal of Plant Protection 9: 401−408.

Khosravi, R., J.J. Sendi, A. Zibaee, & M.A. Shokrgozar. 2015. Virulence of Four Beauveria bassiana (Balsamo) (Ascomycete: Hypocreales) Isolates on Rose Sawfly, Arge rosae under Laboratory Condition. Journal of King Saudy University Science 27: 49−53.

Korada, R.R., S.K. Naskar, M.S. Palaniswami, & R.C. Ray. 2010. Management of Sweet Potato Weevil Cylas formicarius (Fab.): An Overview. Journal of Root Crops 36: 14−26.

Krishna, A.R. & H. Bhaskar. 2013. Ovicidal and Adulficidal Effect of Acaropathogenic Fungi, Neem Oil and New Acaricide Molecules on Tetranychus urticae Koch. Entomology 38: 177−182.

Kumano, N., T. Kuriwada, K. Shiromoto, D. Haraguchi, & T. Kohama. 2011. Prolongation of the Effective Copulation Period by Fractionated-Dose Irradiation in the Sweet Potato Weevil Cylas formicarius. Entomologia Experimentalis et Applicata 141: 129–137.

Leng, H. & G.V.P. Reddy. 2012. Bioactive of Selected Eco-Friendly Pesticides against Cylas formicarius (Coleoptera: Brentidae). Florida Entomology 95: 1040−1047.

Lopes, R.B., M. Michereff-Filho, M.S. Tigano, & P.M. Oliveira. 2011. Virulence and Horizontal Transmission of Selected Brazilian of Beauveria bassiana against Cosmopolites sordidus under Laboratory Conditions. Bulletin of Insectology 64: 201−208.

Lord, J.C. 2009. Beauveria bassiana Infection of Eggs of Stored-Product Beetles. Short Communication 39: 155−156.

Maingi, D., S. Sivasupramaniam, W. Renee, G. Brown, & F. William. 2002. This Persentation is of Student Competition Ten-Minute Papers, Section B. Physiology, Biochemistry, Toxicology and Molecular Biology.http://esa.confex.com/esa/3002/techpro gram/paper_8703.htm, modified 4/9/18.

Malekan, N., B. Hatami, R. Ebadi, A. Akhavan, & R. Radjabi. 2015. Evaluation of Entomopathogenic Fungi Beauveria bassiana and Lecanicillium muscarium on Different Nymphal Stages of Green House Condition. Biharean Biologist 9: 108−112.

Mwamburi, L.A., M.D. Laing, & R.M. Miller. 2015. Effect of Surfactants and Temperature on Germination and Vegetative Growth of Beauveria bassiana. Journal of Microbiology 46: 67−74.

Nisha, S.M., A. Jebanesan, & C.M. Kumar. 2013. Ovicidal Activity of Entomopathogenic Fungi Beauveria bassiana and Metarhizium anisopliae against Dengue Vector Aedes aegypti (Diptera: Culicidae). Insect Environment 19: 195−196.

Okonya, J.S. & J. Kroschel. 2013. Incidence and Damage by the Sweet Potato Butterfly (Acroea acerata Hew.) and the African Sweet Potato Weevils (Cylas spp.) a Cross an Altitude Gradient in Kabale District, Uganda. International Journal of Agricultural Science 3: 814−824.

Peter, C. 2015. Bioactive Metabolites of an Isolate of the Entomopathogenic Fungus Beauveria bassiana. Thesis, Lincoln University. 282 p.

Prayogo, Y.  2012. Virulensi Beberapa Isolat Cendawan Entomopatogen Beauveria bassiana (Balsamo) Vuill. untuk Mengendalikan Penggerek Ubijalar Cylas formicarius, p. 738−754. In A. Widjono, Hermanto, N. Nugrahaeni, A.A. Rahmianna, Suharsono, F. Rozi, E. Ginting, A. Taufiq, A. Harsono, Y. Prayogo & E. Yusnawan (eds.), Prosiding Seminar Nasional Hasil Penelitian Tanaman Aneka Kacang dan Umbi Tahun 2011. Pusat Penelitian dan Pengembangan Tanaman Pangan, Badan Penelitian dan Pengembangan Pertanian, Malang, November 15, 2011.

Prayogo, Y. 2013. Toksisitas Cendawan Beauveria bassiana Vuill. (Balsamo) terhadap Telur dan Larva Penggerek Ubijalar Cylas formicarius (Coleoptera: Curculionidae), p. 669−681. In N. Saleh, A.  Harsono,  N.  Nugrahaeni, A. A. Rahmianna, Sholihin, M. Yusuf, Heriyanto, I. K. Tastra, M. M. Adie, Hermanto & D. Harnowo (eds.), Prosiding Seminar Nasional Hasil Penelitian Tanaman Aneka Kacang dan Umbi Tahun 2013. Pusat Penelitian dan Pengembangan Tanaman Pangan, Badan Penelitian dan Pengembangan Pertanian, Malang, May 22, 2013.

Prayogo, Y. 2016. Proses Pembuatan Biopestisida Berbahan Aktif Cendawan Entomopatogen Beauveria bassiana dan Komposisinya. 16 p. Paten No.: P00201605992.

Quesada-Moraga, E., C. Lopez-Diaz, & B.B. Landa. 2014. The Hidden Habitat of the Entomopathogenic Fungus Beauveria bassiana: First Demonstration of Vertical Plant Transmission. Public Library of Science 9: 1−6.

Rashki, M. & A. Shirvani. 2013. The Effect of Entomopathogenic Fungus Beauveria bassiana on Life Table Parameters and Behavioural Response of Aphis gossypii. Bulletin of Insectology 66: 85−91.

Reddy, G.V.P., N. Gadi, & A.J. Talanao. 2012. Efficient Sex Pheromone Trapping: Catching the Sweetpotato Weevil Cylas formicarius. Journal of Chemical Ecology 38: 846−853.

Reddy, G.V.P., Z. Zhoo, & R.A. Humber. 2014. Laboratory and Field Efficacy of Entomopathogenic Fungi for the Management of the Sweet Potato Weevil, Cylas formicarius (Coleoptera: Brentidae). Journal of Invertebrate Pathology 122: 10−15.

Rina, Y. & H.  Syahbudin. 2013. Zona Kesesuaian Lahan Rawa Pasang Surut Berbasis Keunggulan Kompetitif Komoditas. Socio Economic Agribusiness Journal 10: 103−117.

Seow-Mun, H. & L. Min-Yang. 2015. An Insight into Sweet Potato Weevils Management: A Review. Psyche: A Journal of Entomology 13: 83−91.

Shams, G., M.H. Safaralizadeh, S. Imani, M. Shoja, & S. Aramidah. 2011. A laboratory Assessment of the Potential of the Entomopathogenic Fungi Beauveria bassiana (Beauvarin C) to Control Callosobruchus maculatus (F.) (Coleoptera: Brucidae) and Sitophilus granaries (L.) (Coleoptera: Curculionidae). African Journal of Microbiology Research 10: 1192−1196.

Stafford, K.C. & S.A. Allan. 2010. Field Application of Entomopathogenic Fungi Beauveria bassiana and Metarhizium anisopliae F52 (Hypocreales: Clavicipitaceae) for the Control of Ixodes scapularis (Acari: Ixodidae). Journal of Medical Entomology 47: 1107−1115.

Stevenson, P.C., H. Muyinza, & D.R. Hall. 2009. Chemical Basis for Resistance in Sweet Potato Ipomoea batatas to the Sweet Potato Weevil Cylas puncticollis. Pure and Applied Chemistry 81: 141–151.

Svedese, V.M., E.AdeL. Lima, & A.L.F. Porto. 2013. Horizontal Transmission and Effect of the Temperature in Pathogenicity of Beauveria bassiana against Diatraea succharalis (Lepidoptera: Crambidae). Brazilian Archives of Biology and Technology 56: 413−419.

Swiadikarta, D.A. 2012. Teknologi Pengelolaan Lahan Rawa Berkelanjutan: Studi Kasus Kawasan Ex PLG Kalimantan Tengah. Jurnal Sumberdaya Lahan 6: 45−54.

Swiergiel, W., N.V. Meyling, M. Porcei, & B. Rament. 2015. Soil Application of Beauveria bassiana GHA against Apple Sawly Haplocampa testudinea (Hymenoptera: Tenthredinidae): Field Mortality and Fungal Persistence. Insect Science 23: 854−868.

Taye, A. & E. Tadesse. 2014. Screening Chemical Pesticides for the Management of Sweet Potato Weevil Cylas formicarius. International Journal of Advance Agricultural Research 2: 48−57.

Thangavel, B., K. Palaniappan, P. Kim, M. Subbarayalu, & R. Malhalym. 2013. Pathogenicity, Ovicidal Action, and Median Lethal Concentrations (LC 50) of Entomopathogenic Fungi against Exotic Spiraling Whitefly Aleurodicus disperses Russel. Journal of Pathogens 2013: 1−7.

Wahyunto, R.S, & A. Mulyani. 2011. Sebaran Lahan Gambut di Indonesia, p. 15−26. In N.L. Nurida et al. (eds.), Balai Penelitian Tanah. Badan Penelitian dan Pengembangan Pertanian. Kementerian Pertanian, Bogor.

Wahyunto, R.S., K. Nugroho, & M. Sarwani. 2012. Inventarisasi dan Pemetaan Lahan Gambut di Indonesia, p. 466. In E. Husein, M. Anda, M. Noor, Mamat HS, Maswar, A. Fahmi & Y. Sulaeman (eds.), Prosiding Seminar Nasional Pengelolaan Lahan Gambut Berkelanjutan. Badan Penelitian dan Pengembangan Pertanian. Kementerian Pertanian, Bogor, May 4, 2012.

Wang, Y., C. Wu, T. Cang, L. Yang, W. Yu, X. Zhao, O. Wang, & L. Cai. 2014. Toxicity Risk of Insecticides to the Insect Parasitoid Trichogramma evanescens Westwood (Hymenoptera: Trichogrammatidae). Pest Management Science 70: 398−404.

Wu, S., Y. Gao, X. Xu, D. Wang, J. Li, H. Wang, E. Wang, & Z. Lei. 2015. Feeding on Beauveria bassiana Treated Frankliniella occidentalis Causes Negative Effects on the Predatory Mite Neoseiulus barkeri. Scientific Reports 5: 1−12.

Zhang, L., W.B. Shi, M.G. Feng. 2013. Histopathological and Molecular Insights into the Ovicidal Activities of Two Spotted Spider Mites. Journal of Invertebrate Pathology 117: 73−78.

Zimmerman, T.W., C. Montilla, & S.M.A. Crossman. 2016. Sweet Potato Cultivar and Harvest Date Influence on Damage. Acta Horticulturae (International Society for Horticultural Science) 1118: 153−156.

DOI: https://doi.org/10.22146/jpti.32752

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