* Corresponding Author
Molecular Identification of Begomovirus Infecting Angled Luffa
https://doi.org/10.22146/jpti.31073 Alvina Clara Giovanni(1*), Sedyo Hartono(2), Sri Sulandari(3), Susamto Somowiyarjo(4)
(1) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281
(2) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281
(3) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281
(4) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281
(*) Corresponding Author
Abstract
Begomovirus was reported as one of the most aggressive and destructive viruses on several commercial crops, including cucurbits in Indonesia. Plants that infected with Begomovirus show the mosaic symptom on the leaves, change in leaf shape, stunts, change in color and shape of fruit. It was recently observed in cultivated angled luffa [Luffa acutangula (L.) Roxb] around Yogyakarta and Central Java. The aim of this research was to identify the virus by using Polymerase chain reaction (PCR). The result of Begomovirus amplification from the total DNA samples amplification using primer Krusty-Homer showed that DNA of Begomovirus from angled luffa was amplified at ~580bp. The DNA sequencing of angled luffa’s leaf isolate GD1 had 97.8% homology with SCLV-China isolate MC1. However, amplification of DNA seed samples using the same primer showed negative result. It was concluded that Begomovirus was not a seed borne virus. This is the first molecular report on the occurence of Begomovirus in angled luffa in Yogyakarta.
Keywords
Full Text:
PDFReferences
Akin, H. M. 2006. Virologi Tumbuhan. Kanisius, Yogyakarta. 183 p.
Ariyanti, N. A. 2012. Mekanisme Infeksi Virus Kuning Cabai (Pepper yellow leaf curl virus) dan Pengaruhnya terhadap Proses Fisiologis Tanaman Cabai,p. 682-686. Seminar Nasional IX Pendidikan Biologi FKIP UNS, Surakarta-Central Java.
Daryono, B. S & K. T. Natsuaki. 2009. Survey the Occurrence of Viruses Infecting Cucurbits
in Yogyakarta and Central Java. Jurnal Perlindungan Tanaman Indonesia 15: 83- 89.
Fauquet, C. M. & J. Stanley. 2003. Geminivirus Classification and Nomenclature: Progress and Problems. Annals of Applied Biology 142: 165-189.
Febria, D. 2015. Karakterisasi Virus Penyebab Penyakit Tanaman Cabai (Capcisum sp.) pada Tiga Kondisi Geografis. Tesis. Bioteknologi Universitas Gadjah Mada, Yogyakarta. 68 p.
Giovanni, A. C. 2017. Identifikasi Molekuler Begomovirus pada Gambas (Luffa acutangula). Skripsi. Fakultas Pertanian, Universitas Gadjah Mada, Yogyakarta. 65 p.
Julijantono, I. 2006. Progress Report of Melon Lines Screening to Geminivirus. PT Benih Inti Suburintani (BISI), Kediri. 8 p.
Kandito, A. 2017. Keragaman Biotipe Bemisia tabaci pada Beberapa Jenis Tumbuhan Inang di Daerah Endemik Begomovirus. Skripsi. Fakultas Pertanian, Universitas Gadjah Mada, Yogyakarta. 34 p.
Kil, E. J., S. Kim, Y. J. Lee, H. S. Byun, J. Park, H. Seo. C. S. Kim, J. K. Shim, J. H. Lee, J. K. King, A. M. Q., M. J. Adams, E. B. Carstens, & E. J. Lefkowitz. 2012. Virus Taxonomy Classification and Nomenclature of Viruses. Elsevier, London. 1338 p.
Kumar, S., G. Stecher, & K. Tamura. 2016. MEGA 7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Molecular Biology and Evolution 33: 1870-1874.
Lovisolo, O. 1980. Virus and Viroid Disease of Cucurbits. Acta Horticulturae Vegetable Viruses 88: 33-82.
Matthews, R. E. F. 1970. Plant Virology. Academic Press, Inc., London. 835 p.
Milne, K.S. & R.G. Grogan. 1969. Characterization of Watermelon Mosaic Virus Strains by Serology and Other Properties. Phytopathology 59: 809-818.
Mushtaq, S., F. Shamin, M. Shafique, & M.S. Haider. 2014. Effect of Whitefly Transmitted Geminiviruses on Physiology of Tomato (Lycopersicon esculentum L.) and Tobacco (Nicotiana benthamiana L.) Plants. Journal of Natural Science Research 4: 109-118.
Nene, Y. L. 1973. Viral Diseases of Some Warm Weather Pulse Crops in India. Plant Disease Reporter 57: 463–467.
Ohtsu, Y., N Sako, & S. Somowiyarjo. 1985. Zucchini Yellow Mosaic Virus Isolated from Pumpkin in Miyako and Yaeyama Islands, Okinawa, Japan. Annals of the Phytopathological Society of Japan 51: 234-237.
Reddy, R.C.V., J. Colvin, V. Muniyappa, & S. Seal. 2005. Diversity and Distribution of Begomoviruses Infecting Tomato in India. Archives of Virology 150: 845-867.
Revill, P.A., C.V. Ha, S.C. Porchun, M.T. Vu, & J.L. Dale. 2003. The Complete Nucleotide Sequence of Two Distinct Geminiviruses Infecting Cucurbits in Vietnam. Archives of Virology 148: 1523-1541.
Rusli, E. S., S. H. Hidayat, R. Suseno & B. Tjahjono. 2000. Virus Gemini pada Cabai: Variasi Gejala dan Studi Cara Penularan. Buletin. Hama dan Penyakit Tumbuhan 11: 126-131
Sulandari, S. H. Hidayat, R. Suseno, H. Jumanto, & Sosromarsono. 2001. Keberadaan Virus Gemini pada Cabai di DIY, p. 22-24. In Prosiding Kongres Nasional dan Seminar Ilmiah PFI ke XVI, Bogor-West Java, Agustus 22-24, 2001.
Sulandari, S., R. Suseno, S.H. Hidayat, J. Harjosudarmo, & S. Sosromarsono. 2006. Deteksi dan Kajian Kisaran Inang Virus Penyebab Penyakit Daun Keriting Kuning Cabai. HAYATI Journal of Biosciences 13: 1-6.
Varma, A., A. K. Dhar, & B. Mandal. 1992. MYMV Transmission and Control in India, p. 8–27. Publication No. 92–373. In S.K. Green & D. Kim (eds.), Mungbean Yellow Mosaic Disease: Proceedings of an International Workshop, Bangkok, July 2-3, 1991. Asian Vegetable Research and Development Center (AVRDC), Shanhua, Tainan, Taiwan.
Webb, R.E. 1965. Luffa acutangula for Separation and Maintenance of Watermelon Mosaic Virus 1 free of Watermelon Mosaic Virus 2. Phytopathology 55: 1379-1380.
Wilisiani, F., S. Somowiyarjo, & S. Hartono. 2014. Identifikasi Molekuler Virus Penyebab Penyakit Daun Keriting Isolat Bantul pada Melon. Jurnal Perlindungan Tanaman Indonesia 18: 47-54.
Zitter, T. A., D. L. Hopkins, & C. E. Thomas. 1996. Compendium of Cucurbit Disease. The American Phytopathological Society, Minnesota, USA. 220 p.
DOI: https://doi.org/10.22146/jpti.31073
Article Metrics
Abstract views : 1589
| 
views : 1329
Refbacks
- There are currently no refbacks.
Copyright (c) 2020 Jurnal Perlindungan Tanaman Indonesia
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print), ISSN 2548-4788 (online) is published by the Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
View website statistics