Interaksi Meloidogyne incognita dan Fusarium solani pada Penyakit Kuning Lada

https://doi.org/10.22146/jpti.29760

Suryanti Suryanti(1*), Bambang Hadisutrisno(2), Mulyadi Mulyadi(3), Jaka Widada(4)

(1) Departemen Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Gadjah Mada Jln. Flora 1, Bulaksumur, Sleman, Yogyakarta 55281
(2) Departemen Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Gadjah Mada Jln. Flora 1, Bulaksumur, Sleman, Yogyakarta 55281
(3) Departemen Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Gadjah Mada Jln. Flora 1, Bulaksumur, Sleman, Yogyakarta 55281
(4) Departemen Mikrobiologi Pertanian, Fakultas Pertanian, Universitas Gadjah Mada Jln. Flora 1, Bulaksumur, Sleman, Yogyakarta 55281
(*) Corresponding Author

Abstract


Pepper yellowing disease is one of the most important disease of pepper causing the decrease of pepper production. This research was conducted in the screen house and laboratory to determine the major causal agent of leaf yellowing disease of pepper. Meloidogyne incognita and Fusarium solani were isolated from pepper plantation in West Kalimantan. Pepper seedlings Natar 1 cultivars were planted in sterilized soil collected from pepper plantation in Bengkayang, West Kalimantan. Five months-old seedling were inoculated with M. incognita (1000 larvae of 2nd stadium/plant) and F. solani (50 ml of spore suspension with spore density of 106 spore/ml) in several combinations of time inoculation, i.e., F. solani  and then M. incognita, M. incognita and then F. solani, M. incognita together with F. solani, M. incognita only, and F. solani only. The parameters observed were the development of leaf yellowing disease every weeks for five months. The number of gall, and population M. incognita were observed at the end of the observation. The result showed that when M. incognita was inoculated  to the roots followed by F. solani, the disease severity and the percentage of plant diseases were higher than those which were infected with F. solani or M. incognita alone. The higher population densities of M. incognita and a number of root gall, had observed on plants inoculated by M. incognita combined with F. solani than plants inoculated by M. incognita and F. solani alone. Interaction between M. incognita and F. solani as caused of leaf yellowing disease of pepper was synergistic reaction.

 

Intisari


Penyakit kuning lada merupakan salah satu penyakit penting pada lada yang mengakibatkan terjadinya penurunan produksi lada di Indonesia. Penelitian ini bertujuan untuk mengetahui peran Meloidogyne incognita dan Fusarium solani sebagai penyebab penyakit kuning lada. Penelitian yang dilakukan rumah kasa dan laboratorium. Meloidogyne incognita dan Fusarium solani diisolasi dari pertanaman lada di Kalimantan Barat. Penelitian dilakukan dengan menggunakan bibit lada kultivar Natar 1 berumur 5 bulan, dan diinokulasi dengan M. incognita sebanyak 1000 larva stadium 2 dan 50 ml suspensi mikrokonidium F. solani dengan kerapatan 106/ml. Perkembangan gejala penyakit diamati setiap minggu selama 5 bulan, dan pada akhir pengamatan dilakukan penghitungan jumlah puru dan populasi M. incognita. Hasil penelitian menunjukkan bahwa apabila M. incognita menginfeksi akar dan selanjutnya diikuti dengan infeksi oleh F. solani, tingkat keparahan penyakit dan persentase tanaman sakit lebih tinggi dibandingkan dengan infeksi oleh F. solani atau M. incognita secara terpisah. Populasi M. incognita dan jumlah puru akar pada tanaman yang diinokulasi dengan M. incognita bersama-sama dengan F. solani lebih tinggi dibandingkan pada tanaman yang diinokulasi dengan M. incognita atau F. solani secara terpisah. Interaksi antara M. incognita dan F. solani dalam menyebabkan penyakit kuning lada adalah bersifat sinergis.


Keywords


Fusarium solani; Meloidogyne incognita; pepper yellowing disease; penyakit kuning lada

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References

Anonim. 1993. Baku Operasional Pengendalian Terpadu Penyakit Kuning pada Lada. Direktorat Bina Perlindungan Tanaman Perkebunan. Dirjen Perkebunan. Departemen Pertanian, Jakarta. 14 p.

Anonim. 2012. Pedoman Teknis Rehabilitasi dan Perluasan Tanaman Lada. Direktorat Jenderal Perkebunan. Kementerian Pertanian RI, Jakarta. 45 p.

Anonim. 2013. IPC Market Review 2012. http:// www. peppertrade.com, diakses 30/05/2014.

Anonim. 2016. Statistik Perkebunan Indonesia Lada. Direktorat Jenderal Perkebunan Indonesia, Jakarta. 48 p.

Byrd, D.W., Jr., T. Kirkpatrick, & K.R. Barker. 1983. An Improved Technique for Clearing and Staining Plant Tissue for Detection of Nematodes. Journal of Nematology 15: 142−143.

Castagnone-Sereno, P. & A. Kermarrec. 1991. Invasion of Tomato Roots and Reproduction of Meloidogyne incognita as Affected by Raw Sewage Sludge. Supplement to Journal of Nematology 23: 724−728.

Curtis, R.H.C. 2008. Plant-Nematode Interactions: Environmental Signals Detected by the Nematode’s Chemosensory Organs Control Changes in the Surface Cuticle and Behaviour. Parasite 15: 310–316.

De Boer, J.M.. E. L. Davis, R. S. Hussey, H. Popeijus, G. Smant, & T. J. Baum. 2002. Cloning of a Putative Pectate Lyase Gene Expressed in the Subventral Esophageal Glands of Heterodera glycines. Journal of Nematology 34: 9–11.

Desaeger, J.A. & A.S. Csino. 2006. Root-knot Nematode Management in Double-cropped Plasticulture Vegetables. Journal of Nematology 38: 59–67.

El- Khallal, S.M. 2007. Induction and Modulation of Resistance in Tomato Plants against Fusarium Wilt Disease by Bioagent Fungi (Arbuscular Mycorrhiza) and/or Hormonal Elicitors (Jasmonic Acid & Salicylic Acid): 1- Changes in Growth, Some Metabolic Activities and Endogenous Hormones Related to Defence Mechanism. Australian Journal of Basic & Applied Science 1: 691−705.

France, R.A., & G.S. Abawi. 1994. Interaction between Meloidogyne incognita and Fusarium oxysporum f. sp. phaseoli on Selected Bean Genotypes.  Journal of Nematology 26: 467–474.

Hanounik, S.B.  & W.W. Osborne. 1975. Influence of Meloidogyne incognita on the Content of Amino Acids and Nicotine in Tobacco Grown under Gnotobiotic Conditions. Journal of Nematology 4: 332–336.

Hooper , D.J., 1985. Extraction of Free-living Stages from Soil, pp. 5–30. In J.F. Southey (ed.) Laboratory Methods for Work with Plant and Soil Nematodes. Ministry of Agriculture, Fisheries and Food, London.

Jin, H., G.L. Hartman, C.D. Nickell & J.M. Widholm. 1996. Characterization and Purification of a Phytotoxin Produced by Fusarium solani, Causal Agent of Soybean Sudden Death Syndrome. Phytpathology 86: 277−282.

Koshy, P.K., S.J. Eapen & R. Pandey. 2005. Nematode Parasies of Spices, Condiments and Medicinal Plants, pp. 751−792. In M. Luc, R.A. Sikora, & J. Bridge (eds.), Plant Parasitic Nematodes in Subtropical and Tropical Agriculture. 2nd ed. CABI Publ. Wageningen, Netherland.

Lamondia, J.A. 1992. Predisposition of Broadleaf Tobacco to Fusarium Wilt by Early Infection with Globodera tabacum tabacum or Meloidogyne hapla. Journal of Nematology 24: 425−431.

Meena, K.S., S.A. Ramyabharathi, T. Raguchander, & E.I. Jonathan. 2016. Interaction of Meloidogyne incognita and Fusarium oxysporum in Carnation and Physiological Changes Induced in Plants Due to the Interaction. SAARC Journal of Agriculture 14: 59−69.

Mulyadi. 2009. Nematologi Pertanian. Gadjah Mada University Press, Yogyakarta. 339 p.

Mustika, I. 1990. Studies on the Interactions of Meloidogyne incognita, Radopholus similis and Fusarium solani on Black Pepper (Piper nigrum L.) Disertation. Landbouwuniversitet, Wageningen. (Unpublished).

Nemec, S., D. Phelps, & R. Baker. 1989. Effects of Dihydrofusarubin and Isomarticin from Fusarium solani in Carbohydrate Status and Metabolism of Rough Lemon Seedlings. Phytopathology 79: 700−705.

Suryanti, B. Hadisutrisno, Mulyadi, & J. Widada. 2013. Survei Sebaran Penyakit Kuning Lada dan Patogen yang Berasosiasi. Jurnal Budidaya Pertanian 9: 54−57.

Suryanti, B. Hadisutrisno, Mulyadi, & J. Widada. 2015. Identifikasi Fusarium dan Nematoda Parasitik yang Berasosiasi dengan Penyakit Kuning Lada di Kalimantan Barat. Jurnal Perlindungan Tanaman Indonesia 19: 19−26.

Wintermans, J. F. G. M. & A. DeMots. 1965. Spectro-photometric Characteristics of Chlorophyll ‘a’ and ‘b’ and their Pheophytins in Ethanol. Biochimica et Biophysica Acta 109: 448−453.

Wu, H., X.Yin, D. Liu, N. Ling, W. Bao, R. Ying, Y. Zhu, S. Guo, & Q. Shen. 2008. Effect of Fungal Fusaric Acid on the Root and Leaf Physiology of Watermelon (Citrullus lanatus) Seedlings. Plant Soil 308: 255–266.



DOI: https://doi.org/10.22146/jpti.29760

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