Resistance Level of Plutella xylostella L. (Lepidoptera: Plutellidae) on Cypermethrin in the Regency of Kupang

https://doi.org/10.22146/jpti.24750

Noldy R. E. Kotta(1*), Y. Andi Trisyono(2), Arman Wijonarko(3)

(1) Department of Crop Protection, Faculty of Agriculture, UniversitasGadjahMada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281
(2) Department of Crop Protection, Faculty of Agriculture, UniversitasGadjahMada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281
(3) Department of Crop Protection, Faculty of Agriculture, UniversitasGadjahMada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281
(*) Corresponding Author

Abstract


The diamondback moth, Plutella xylostella L. (Lepidoptera: Plutellidae) is one of the major pests of the Brassicaceae family. Presently,  farmers  mostly use  insecticide to control this pest. The improper use of insecticide, however,  may lead to  target pest resistance. Resistance to pyrethroid (cypermethrin) insecticide cases  have been widely reported. This research aimed to know whether the farmers use  cypermethrin based on the recommended practices  and to determine the resistance level of P. xylostella collected from Tarus, Noelbaki, Oesao and Pukdale Villages collected from the Kupang District, East Nusa Tenggara, which were then compared to  the population of Cangkringan (Sleman, Yogyakarta) as a susceptible population. The survey showed that these four locations in Kupang, used insecticides intensively and did the mixing of two or three insecticides. Insecticide application in these four locations was mostly not scheduled as recommended on the label of insecticides. The susceptibility test showed that the RR (resistance ratio) value from Kupang (Tarus = 9.2, Noelbaki = 7.2, Oesao 7.3, and Pukdale = 3.8, respecively) was higher than susceptible population (Cangkringan = 1.0). Therefore, P. xylostella larvae collected from Kupang has been resistant to cypermethrin.

 

Intisari

Plutella xylostella L. merupakan salah satu hama utama pada tanaman Famili Brassicaceae (Cruciferae). Pengendalian dengan insektisida masih menjadi andalan petani di lapangan. Dampak negatif dari insektisida seperti resistensi, resurgensi dan munculnya hama sekunder tidak membuat efek jera karena minimnya pengetahuan tentang dampak negatif tersebut. Resistensi terhadap golongan piretroid sudah banyak dilaporkan. Salah satunya sipermetrin. Tujuan dari penelitian ini untuk melihat cara penggunaan insektisida oleh petani dan tingkat kepekaan dari populasi lapang asal Tarus, Noelbaki, Oesao, dan Pukdale (Kupang, NTT) dibandingkan dengan populasi Cangkringan (Sleman, DIY) terhadap insektisida sipermetrin. Data survei tentang cara penggunaan insektisida oleh petani diketahui bahwa penggunaan insektisida di keempat lokasi tersebut cukup intensif dan terjadi pencampuran insektisida yang tidak kompatibel. Penyemprotan dilakukan secara rutin di Desa Tarus; di Desa Noelbaki dan Oesao ada yang secara rutin dan ada yang kondisional; sedangkan penyemprotan secara kondisional di Desa Pukdale. Data uji kepekaan menunjukkan bahwa P. xylostella asal Kupang telah resisten terhadap sipermetrin dengan nilai RR (rasio resistensisi) tertinggi dari populasi Tarus (9,2), Noelbaki (7,2), Oesao (7,3), Pukdale (3,8) dibandingkan dengan populasi peka asal Cangkringan. 


Keywords


cypermethrin; Plutella xylostella; resistance

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References

Agboyi, L.K., G.K. Ketoh, T. Martin, I. A. Glitho, & M. Tamò. 2016. Pesticide Resistance in Plutella xylostella (Lepidoptera: Plutellidae) Populations from Togo and Benin. International Journal of Tropical Insect Science 36: 204−210.

Branco, M.C. & A.G. Gatehouse. 1997. Insecticide Resistance in Plutella xylostella (L.) (Lepidoptera: Yponomeutidae) in the Federal District, Brazil. Anais da Sociedade Entomológica do Brasil 26: 75−79.

Cloyd, R.A. 2011. Pesticide Mixtures, p. 69−80. In M. Stoytcheva (ed.), Pesticides-Formulations, Effects, Fate. In Tech, Croatia.

Cox, C. 2002. Insecticide Fact Sheet. Journal of Pesticide Reform 16: 15−20.

Ells, J.T. 1992. Pyrethroid Insecticide Induced Alteration in Mammalian Synaptic Membrane Potential. Journal of Pharmacology and Experimental Therapeutics 262: 1173−1181.

Elzinga, R.J. 2004. Fundamentals of Entomology. Prentice Hall, New Jersey, USA. 512 p.

Finney, D.J. 1971. Probit Analysis. 3rd ed. Cambridge University Press, UK. 333 p.

Furlong, M.J., H. Spafford, P.M. Ridland, N.M. Endersby, O.R. Edwards, G.J. Baker, M.A. Keller, & C.A. Paull. 2008. Ecology of Diamondback Moth in Australian Canola: Landscape Perspectives and the Implications for Management. Australian Journal of Experimental Agriculture 48: 1494− 1505.

Gong, Y.J., Z.H. Wang, B.C. Shi, Z.J. Kang, L. Zhu, G.H. Jin, &S.J. Weig. 2013. Correlation between Pesticide Resistance and Enzyme Activity in the Diamondback Moth, Plutella xylostella. Journal of Insect Science 13:135.

Insecticide Resistance Action Committee [IRAC]. 2012. International Insecticide MixtureStatement. Insecticide Resistance Action Committee. Indianapolis, AS. http://www.iraconline.org/ content/uploads/IRAC_Mixture_Statement_ v1.0_10Sept12.pdf, modified 7/4/17.

Jiang, T., S. Wu, T. Yang, C. Zhu, & C. Gao. 2015. Monitoring Field Populations of Plutella xylostella (Lepidoptera: Plutellidae) for Resistance to Eight Insecticides in China. Florida Entomologist 98: 65−73.

Kazachkova, N.I. 2007. Genotype Analysis and Studies of Pyrethroid Resistance of the Oilseed Rape (Brassica napus) Insect Pest-Pollen Beetle (Meligethes aeneus). Doctoral thesis. Swedish. University of Agricultural Sciences, Uppsala. https://pub.epsilon.slu.se/1329/1/nadiyakazach kova_200711.pdf., modified 17/05/17.

Kerns, D.L., J.C. Palumbo, & T. Tellez. 1998. Resistance of Field Strains of Beet Armyworm (Lepidoptera: Noctuidae) from Arizona and California to Carbamate Insecticides. Journal of Economic Entomology 91: 1038−1043.

Listyaningrum, W., Y.A. Trisyono, & A. Purwantoro. 2003a. Selection toward Resistance to Deltametrin in Plutella xylostella. Agrosains 16: 135−136.

Listyaningrum, W., Y.A. Trisyono, & A. Purwantoro. 2003b. Inheritance of Plutella xylostella Resistant to Deltamethrin. Jurnal Perlindungan Tanaman Indonesia. 9: 28−34.

Maa, C.J.W., H.J. Wang, & C.F. Liu. 2001. Variation in Carboxylesterase Frequency and Insecticide Resistance of Plutella xylostella (L.) as a Response to Environmental Gradients, p. 333−344. In N.M. Endersby & P.M. Ridland (eds.), Proceedings of the 4th International Workshop on the Management of Diamondback Moth and other Crucifer Pests. Melbourne, Australia, November 26−29, 2001.

Nuryanti, N. P. & Y. A. Trisyono. 2002. The Susceptibility of Plutella xylostella Field Population Collected from Central Java and Yogyakarta to Bacillus thuringiensis. Agrosains 15: 1−5.

Pracaya. 1993. Hama dan Penyakit Tanaman. Penebar Swadaya, Jakarta. 417 p.
Qian L., G.C. Cao, J.X. Song, Q. Yin, & Z.J. Han. 2008. Biochemical Mechanisms Conferring Cross-Resistance between Tebufenozide and Abamectin in Plutella xylostella. Pesticide Biochemistry and Physiology 91: 175−179.

Sayyed, A.H. & D.J. Wright. 2006. Genetics and Evidence for an Esterase-Associated Mechanism of Resistance to Indoxacarb in a Field Population of Diamondback Moth (Lepidoptera: Plutellidae). Pest Management Science 62: 1045−1051.

Setiawati, W. 2000. Controlling of Diamond Backmoth (Plutella xylostella L.) and Cabbage Head Caterpillar (Crocidolomia binotalis Zell.) by Using Spinosad 25 SC and its Effect on Parasitoid Diadegma semiclausum Hellen. Journal of Horticulture 10: 30–39.

Shelton, A.M., J.L. Robertson, J.D. Tang, C. Perez, S.D. Eigenbrode, H.K. Preisler, W.T. Wilsey & R.J. Cooley. 1993. Resistance of Diamondback Moth (Lepidoptera: Plutellidae) to Bacillus thuringiensis subspecies in the Field. Journal of Economic Entomology 86: 697−705.

Siegfried, B.D., T.T. Vaughn, & T. Spencer. 2005. Baseline Susceptibility of Western Corn Rootworm (Coleoptera; Crysomelidae) to Cry 3Bb1 Bacillus thuringiensis. Journal of Economic Entomology 98: 1320−1324.

Srigiriraju, L., P.J. Semtner, T.D. Anderson, & J.R. Bloomquist. 2009. Esterase-based Resistance in the Tobacco-adapted form of the Green Peach Aphid, Myzuspersicae (Sulzer) (Hemiptera: Aphididae) in the Eastern United States. Archives of Insect Biochemistry and Physiology 72: 105−123.

Sudarwohadi, S. 1975. Hubungan antara Waktu Tanam Kubis dan Dinamika Populasi Plutella maculipennis Curt. dan Crocidolomia binotalis Zell. Buletin Penellitian Hortikultura 3: 3−14.

Talekar, N.S. & A.M. Shelton. 1993. Biology, Ecology, and Management of the Diamondback Moth. Annual Review of Entomology 38: 275− 301.

Trisyono, Y.A. & M.G. Chippendale. 2002. Susceptibility of Field-collected Populations of the Southwestern Corn Borer, Diatraea grandiosella, to Bacillus thuringiensis. Pest Management Science 58: 1022−1028.

Udiarto, B.K. & S. Sastrosiswojo. 1997. Selektivitas Beberapa Jenis Insektisida terhadap Larva Plutella xylostella L. dan Parasitoid Imago Diadegma semiclausum Hellen. Jurnal Hortikultura 7: 810−817.

Untung, K. 2006. Pengantar Pengelolaan Hama Terpadu. UGM Press, Yogyakarta. 348 p.
Ware, G.W. & D.M. Whitacre. 2004. The Pesticide Book. Freeman and Co., USA. 386 p.

Winarto, L., & D. Nazir. 2004. Teknologi Pengendalian Hama Plutella xylostella dengan Insektisida dan Agensia Hayati pada Kubis di Kabupaten Karo. Jurnal Pengkajian dan Pengembangan Teknologi Pertanian 7: 27−33.

Winasa, I.W. & S. Herlinda. 2003. Population of Diamondback Moth, Plutella xylostella L. (Lepidoptera:Plutellidae), and its Damage and Parasitoids on Brassicaceous Crops, p. 310−314 In Proceeding International Seminar on Organic Farming and Sustainable Agriculture in the Tropics and Subtropics. Sriwijaya University Publishers, Inc., Palembang.

Yu, J., 1993. Inheritance of Insecticide Resistance and Microsomal Oxidases in the Diamondback Moth (Lepidoptera: Yponomeutidae). Journal of Economic Entomology 86: 680−683.



DOI: https://doi.org/10.22146/jpti.24750

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