Morphological and Molecular Characters of Mimegralla spp. (Diptera: Micropezidae) on Zingiberaceae in Central Java

https://doi.org/10.22146/jpti.17537

Rahma Widyastuti(1*), Suputa Suputa(2), Nugroho Susetya Putra(3),

(1) Center for Research and Development of Medicinal Plants and Traditional Medicine, Jln. Raya Lawu No. 11, Tawangmangu, Karanganyar, Central Java 57792
(2) Department of Plant Pest and Disease, Faculty of Agriculture, Universitas Gadjah Mada, Jln. Flora 1, Bulaksumur, Sleman, Yogyakarta 55281
(3) Department of Plant Pest and Disease, Faculty of Agriculture, Universitas Gadjah Mada, Jln. Flora 1, Bulaksumur, Sleman, Yogyakarta 55281
(*) Corresponding Author

Abstract


Rhizome fly, Mimegralla sp. (Diptera: Micropezidae) is a major pest on Zingiberaceae plants. Some of those fly species have been reported attacking some of Zingiberaceae plants, but in Indonesia, only one species, Mimegralla coeruleifrons has been reported as being a pest. Since Indonesia has many species of Zingiberaceae plants, it may raise a prediction that more than one species of Mimegralla was found on this plants. Therefore, a taxonomic research on the species of rhizome flies to find the species other than M. coerulifrons on Zingiberaceae plants is urgently required. This study was conducted by using hand-picking method on Mimegralla adult inhabiting Zingiberaceae plants (ginger, turmeric, javanese ginger, and aromatic ginger), and was then identified by using morphological characters and through molecular technique by using mtCO1 gene. The results showed thatM. albimana and M. coeruleifrons found at four zingiberaceae plants were the member of Mimegralla. As a conclusion, these two species have high values of phylogenic relationship (88%) and bootstrap (92).


INTISARI


Lalat rimpang (Mimegralla spp.) adalah hama utama pada beberapa tanaman anggota Family Zingiberaceae. Beberapa spesies lalat rimpang dilaporkan menyerang tanaman Zingiberaceae, di antaranya jahe, kunyit, temulawak, dan kencur. Di Indonesia hanya satu spesies yang pernah dilaporkan menyerang tanaman jahe yaituMimegralla coeruleifrons.Sementara itu, jenis tanaman Zingiberaceae yang ditanam di Indonesia sangat beragam, sehingga muncul dugaan bahwa Mimegralla yang menyerang tanaman Zingiberaceae di Indonesia tidak hanyaM. coeruleifrons saja. Oleh karena itu, penelitian untuk mengidentifikasi spesies-spesies lalat rimpang yang menyerang tanaman Zingiberaceae di Indonesia perlu dilakukan. Penelitian ini dilakukan menggunakan metode pemungutan/penangkapan (hand-picking) imago Mimegralla pada tanaman keempat tanaman Zingiberaceae. Imago yang diperoleh diidentifikasi secara morfologi dan molekuler menggunakan genmtCO1. Hasil penelitian ini menunjukkan bahwa M. albimana dan M. coeruleifrons yang ditemukan pada pertanaman Zingiberaceae merupakan satu genus yang sama yaitu Mimegralla. Hasil identifikasi molekuler menunjukkan bahwa kedua lalat rimpang tersebut mempunyai derajat kekerabatan dengan nilai homologi sebesar 88%, dan nilaibootstrap pada pohon filogeni sebesar 92.

 


Keywords


Mimegralla spp.; molecular; molekuler; morfologi; morphology

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References

Aczel, M.L. 1959. Insects of Micronesia, Diptera: Neriidae and Micropezidae. Bernice P. Bishop Museum, Hawaii. 90 p.

Balfas, R. 2002. Status Lalat Rimpang pada Tanaman Jahe dan Strategi Pengendaliannya (Status of Rhizome Fly at Ginger and their Control). Jurnal Litbangtan (Journal of Research and Development on Agriculture) 21: 32–37.

Balfas, R., Supriadi, N. Karyani, & E. Sugandi. 2000. Serangan Mimegralla coeruleifrons Macquart dan Peranannya dalam Membawa Patogen Bakteri Penyakit Layu (Attack of Mimegralla coeruleifrons Macquart and its Role in Bringing Pathogen of Wilt Disease. Jurnal Penelitian Tanaman Industri (Research Journal of Industrial Plant) 5: 123–127.

Folmer, O., M. Black, W. Hoeh, R. Lutz, & R. Vrijenhoe. 1994. DNA Primers for Amplification of Mitocondrial Cytochrom C Oxidase Subunit I from Diverse Metazoan Invertebrates. Molecular Marine Biology and Biotechnology 3: 294–299.

Ghorpade, S.A., S.S. Jadhav, & D.S. Ajri. 1983. Survey of Rhizome Fly on Turmeric and Ginger in Maharashtra. Journal Maharashtra Agriculture University 8: 292–293.

Ghorpade, S.A., S.S. Jadhav, & D.S. Ajri. 1988. Biology of Rhizome Fly, Mimegralla coeruleifrons Macquart (Micropezidae: Diptera) in India, a Pest of Turmeric and Ginger Crops. Tropical Pest Management 34: 48–51.

Hedges, S.B. 1992. The Number of Replications Needed for Accurate Estimation of the Bootstrap p-Value in Phylogenetic Studies. Molecular Biology and Evolution 9: 366–369.

Hennig, W. 1935. Revision der Tyliden (Dipt., Acalypt.) II Teil: Die außeramerikanischen Taeniapterinae, die Trepidariinae und Tylinae. Allgemeines über die Tyliden. (Fortsetzung). Konowia 14: 192–216.

Joy, P.P., J. Thomas, S. Mathew, & B.P. Skaria. 1998. Zingiberaceous Medicinal and Aromatic Plants. Aromatic and Medicinal Plants Research Station, Odakkali, Asamannoor P.O., Kerala, India. 31 p.

Karmawati, E., B. Baringbing, M. Iskandar, & T.E. Wahyono. 1990. Observasi Lalat Rimpang pada Pertanaman Jahe di K.P. Sukamulya. (Observation of Rhizome Fly on Ginger Plants at Sukamulya Village). Media Komunikasi Penelitian dan Pengembangan Tanaman Industri (Media Communication Research and Development Industrial Crops) 6: 84–86.

Maxwell-Lefroy, H. & F.M. Howlett. 1909. Indian Insect Life-A Manual of the Insects of the Plains (Tropical India). Government Press, Calcutta. 486 p.

Nair, M.R.G.K. 1980. Pests of Ginger and Turmeric, p. 101–103. In M.K. Nair, T. Premkumar, P.N. Ravindran, & Y.R. Sarma (eds.), Proceedings of the National Seminar on Ginger and Turmeric. Calicut, 8–9 April 1980.

Pape, T., V. Blagoderov, & M.B. Mostovski. 1758. Order Diptera Linnaeus. In Z.Q. Zhang (ed.), Animal Biodiversity: An Outline of Higher-Level Classification and Survey of Taxonomic Richness. Zootaxa 3148: 222–229.

Sokal, R.H. & P.A. Sneath. 1963. Principle of Numerical Taxonomy. W.H. Freeman and Co., San Fransisco. 359 p.

Steyskal, G.C. 1964. Larvae of Micropezidae (Diptera) Including the Spesies that Bore Ginger Root. Annal of the Entomological Society of America 57: 292–296.



DOI: https://doi.org/10.22146/jpti.17537

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