* Corresponding Author
Survei Odontoglossum ringspot virus (ORSV) yang Menginfeksi Anggrek Alam Tropis di Indonesia
https://doi.org/10.22146/jpti.16617 Mahfut Mahfut(1*), Budi Setiadi Daryono(2), Tri Joko(3), Susamto Somowiyarjo(4)
(1) Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Lampung, Jln. Soemantri Brojonegoro No.1, Rajabasa, Bandar Lampung 35145.
(2) Fakultas Biologi Universitas Gadjah Mada, Jln. Teknika Selatan, Sekip Utara, Sleman, Yogyakarta 55281.
(3) Fakultas Pertanian Universitas Gadjah Mada, Jln. Flora 1, Bulaksumur, Sleman, Yogyakarta 55281.
(4) Fakultas Pertanian Universitas Gadjah Mada, Jln. Flora 1, Bulaksumur, Sleman, Yogyakarta 55281.
(*) Corresponding Author
Abstract
Natural orchids are one of the important ornamental plants that were cultivated in tropical countries, including Indonesia. Virus infections has been important limiting factor in orchids cultivation because it decreases the orchids quality. Odontoglossum ringspot virus (ORSV) is one of the most reported virus infecting orchids which spread widely in the world. During 2010–2014 surveys of viral infections were conducted in Indonesia. The orchids were found infected by virus, showed symptoms of mosaic, mottle, chlorotic, necrotic, streak, wilting leaf, and ringspot on leaf surface. Detection with serological test DAS-ELISA showed only 11 from 125 samples were infected by ORSV with total incidence of 8,8%. Nine leaf samples of Phalaenopsis sp. were infected, respectively. Thus, it was concluded that Phalaenopsis is orchids genus which is the most abundantly and susceptibly infected by ORSV. The results proved that ORSV have entered and spread widely by infected orchids in orchids landscape (nursery), semi-natural forests (botanical gardens), and natural forest (national park) throughout Indonesia. This is the first report of ORSV infecting natural tropical orchids in Indonesia.
INTISARI
Anggrek alam merupakan salah satu kekayaan flora asli negara tropis, termasuk Indonesia. Keberadaan di habitat aslinya sudah sangat berkurang yang disebabkan kerusakan hutan dan adanya penyakit. Infeksi virus masih menjadi faktor pembatas terpenting dalam budidaya dan pengembangan potensi anggrek alam. Odontoglossum ringspot virus (ORSV) adalah salah satu jenis virus yang dilaporkan paling banyak menginfeksi anggrek serta memiliki penyebaran yang luas di dunia. Selama 2010-2014 telah dilakukan survei lapangan terhadap infeksi virus di Indonesia. Beberapa anggrek yang ditemukan terinfeksi oleh virus menunjukkan gejala berupa mosaik, belang, klorosis, nekrosis, streak, daun layu, dan bercak cincin pada permukaan daun. Deteksi dengan uji serologis DAS-ELISA menunjukkan bahwa 11 dari 125 sampel terinfeksi oleh ORSV dengan total kejadian 8,8%. Masing-masing sembilan dari total sampel daun terinfeksi merupakan Phalaenopsis sp. Hal ini menjelaskan bahwa Phalaenopsis adalah genus anggrek yang paling cocok dan rentan terhadap infeksi ORSV. Hasil penelitian survei kejadian infeksi Odontoglossum ringspot virus (ORSV) membuktikan bahwa virus ini telah masuk dan menyebar secara luas oleh anggrek-anggrek alam tropis yang terinfeksi di pertamanan anggrek (nurseri), hutan semi-alami (kebun raya), dan hutan alam (taman nasional) di seluruh wilayah Indonesia. Ini merupakan laporan pertama mengenai infeksi ORSV terhadap anggrek-anggrek alam tropis di Indonesia.
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Anonim. 2011. Peraturan Presiden Republik Indonesia Nomor 93 Tahun 2011 Tentang Kebun Raya. Jakarta, 12 p.
Bawden, F.C. 1964. Plant Viruses and Virus Diseases: Introduction and Odontoglossum ringspot virus (ORSV), p. 222−240. In T. Kull & J. Arditti (eds.), Orchid Biology: Reviews and Perspectives, VIII: Ronald Press, New York.
Bulpitt, C.J, Y. Li, P.F. Bulpitt, & J. Wang. 2007. The Use of Orchids in Chinese Medicine. Journal of The Royal Society of Medicine 100: 558–563.
Clark, F. & A.N. Adams. 1977. Characteristics of the Microplate Method of Enzyme-Linked Immunosorbent Assay for the Detection of Plant Viruses. Journal of General Virology 34: 475−483.
Daryono, B.S. & K.T. Natsuaki. 2009. Survei Virus yang Menyerang Labu-Labuan di Yogyakarta dan Jawa Tengah. Jurnal Perlindungan Tanaman Indonesia 15: 83–89.
Hossain, M.M. 2011. Review Therapeutic Orchids: Traditional Uses and Recent Advances - An Overview. Fitoterapia 82: 102–40.
Hull, R. 2002. Matthews Plant Virology. 4th ed. Acad. Press, San Diego. 1056 p.
Hull, R. 2009. Comparative Plant Virology: In Introduction to Plant Viruses. Overview of Plant Viruses. 2nd ed. Elsevier Acad. Press, United Kingdom. 400 p.
Inouye, N. & I.W. Gara. 1996. Detection and Identification of Viruses of Orchid in Indonesia. Bulletin of the Earthquake Research Institute 4: 109−118.
Isnawati, L. 2009. Deteksi dan Identifikasi Odontoglossum ringspot virus (ORSV) pada Tanaman Anggrek. Institut Pertanian Bogor, Bogor. 39 p.
Jensen, D.D. & H.A. Gold. 1951. A Virus Ringspot of Odontoglossum Orchid, Symptoms, Transmission and Electron Microscopy. In Lawson & Sahfqat Ali. The Handbook on Orchid Pests and Disease. American Orchid Society 4: 62−100.
Johanis, P., G. Djunaedi, W. Harry, E.N. Rusdy, dan Irawati. 2001. Tumbuhan Langka Indonesia. Puslitbang LIPI, Bogor. 86 p.
Koh, KW, H.C. Lu, & M.T. Chan. 2014. Virus Resistance in Orchids. Plant Science 228: 26−38.
Kumalawati, A.D., S. Abdullah, B.S. Setiadi, & Mahfut. 2011. Study on Genetic Diversity and Conservation of Orchids in Wonosadi Forest, Gunung Kidul Based on Molecular Analysis, p. 72−79. In Y.A. Purwestri, (ed.), International Conference on Biological Science: Advances in Biological Science, Faculty Biology UGM, Yogyakarta. September 23rd−24th 2011.
Lakani, I., G. Suastika, N. Mattjik, and T.A. Damayanti. 2010. Identification and Molecular Characterization of Odontoglosum ringspot vVirus (ORSV) from Bogor, Indonesia. Hayati Journal of Biosciences 17: 101−104.
Navalinskiene, M., J. Raugalas, & M. Samuitiene. 2005. Viral Diseases of Flower Plants 16. Identification of Viruses Affecting Orchids (Cymbidium Sw.). Biologija 2: 29−34.
Paul, H.L. 1975. Odontoglossum ringspot virus. http:// www.dpvweb.net/dpv/showdpv.php?dpvno=155, modified 27/06/16. 155 p. Rukmana, R. 2000. Anggrek Bulan. Penerbit Kanisius, Yogyakarta. 96 p.
Soetopo, L. 2009. Keanekaragaman dan Pelestarian Tanaman Anggrek. Penerbit Citra, Malang. 91 p.
Syahierah, P. 2010. Respon Berbagai Jenis Anggrek (Orchidaceae) terhadap Infeksi Cymbidium Mosaic virus (CyMV) dan Odontoglossum Ringspot Virus ORSV). Institut Pertanian Bogor, Bogor. 39 p.
DOI: https://doi.org/10.22146/jpti.16617
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