* Corresponding Author
Identifikasi Fusarium dan Nematoda Parasitik yang Berasosiasi dengan Penyakit Kuning Lada di Kalimantan Barat
https://doi.org/10.22146/jpti.16019 Suryanti Suryanti(1*), Bambang Hadisutrisno(2), Mulyadi Mulyadi(3), Jaka Widada(4)
(1) Fakultas Pertanian, Universitas Gadjah Mada, Jln. Flora 1, Bulaksumur, Sleman, Yogyakarta 55281
(2) Fakultas Pertanian, Universitas Gadjah Mada, Jln. Flora 1, Bulaksumur, Sleman, Yogyakarta 55281
(3) Fakultas Pertanian, Universitas Gadjah Mada, Jln. Flora 1, Bulaksumur, Sleman, Yogyakarta 55281
(4) Fakultas Pertanian, Universitas Gadjah Mada, Jln. Flora 1, Bulaksumur, Sleman, Yogyakarta 55281
(*) Corresponding Author
Abstract
Pepper (Piper nigrum), known as the “King of Spices” is one of the most important spices. In the international market, Indonesian pepper has high selling value, due to its flavor characteristics. Pepper yellowing disease is one of the most important disease that caused the decrease of pepper production and become the main problem in the cultivation of pepper in West Kalimantan. This research was conducted to determine the major causal agent of leaf yellowing disease of pepper. The Fusarium associated with diseased plant were isolated from the symptomatic plant and nematodes were isolated from the root with leaf yellowing symptom. The Fusarium isolates were cultured on agar medium, and the nematode was cultured on tomato plant. From diseased pepper in West Kalimantan, it was isolated 4 Fusarium isolates and plant parasitic nematode Meloidogyne. The result showed that H isolate of Fusarium was the most virulent isolate and identified asFusarium solani. The Meloidogyne was identified by the female perenial patern.The nematode was identified as Meloidogyne incognita.
INTISARI
Lada (Piper nigrum L.) merupakan salah satu jenis rempah penting yang telah dikenal sebagai “King of Spices”. Di pasar internasional, lada Indonesia mempunyai daya jual tinggi karena cita rasanya yang khas. Salah satu kendala dalam budidaya lada adalah adanya penyakit kuning lada dan sampai saat ini menjadi masalah utama pada pertanaman lada di Kalimantan Barat. Informasi tentang patogen utama yang berinteraksi dengan penyakit kuning lada masih sangat terbatas, sehingga penelitian ini bertujuan untuk mengidentifikasi patogen utama yang berasosiasi dengan penyakit kuning lada. Isolasi Fusarium dilakukan dari batang lada dan isolasi nematoda dilakukan dari akar lada yang bergejala penyakit kuning di Kalimantan Barat. Fusarium hasil isolasi dikulturkan dalam medium agar, sedangkan nematoda hasil isolasi dikulturkan dalam akar tomat. Dari hasil isolasi berhasil didapatkan empat isolat Fusarium dan nematoda Meloidogyne. Identifikasi Fusarium dilakukan secara morfologis dan molekuler, dan identifikasi Meloidogyne dilakukan dengan menggunakan irisan bagian posterior nematoda betina. Dari hasil identifikasi diketahui bahwa patogen yang berasosiasi dengan penyakit kuning lada adalah Fusarium solani dan Meloidogyne incognita.
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Abd-Elsalam, K.A., I.N. Aly, M.A. Abdel-Satar, M.S. Khalil & J.A. Verreet. 2003. PCR Identification of Fusarium Genus Based on Nuclear Ribosomal DNA Sequence Data. African Journal of Biotechnology 2: 82−85.
Anandaraj, M. & Y.R. Sarma. 1995. Diseases of Black Pepper (Piper nigrum L.) and their Management. Journal of Spices and Aromatic Crops 4: 17–23.
Anonim. 2006. Statistik Perkebunan Indonesia 2004−2006: Lada. Direktorat Jenderal Perkebunan Departemen Pertanian, Jakarta. 47 p.
Anonim. 2009. Pepper: Again the ‘Bull Run’ is Expected. Carvy Special Reports. http://www.karvycomtrade.com, diakses 23/2/12.
Anonim. 2012. Pedoman Teknis Rehabilitasi dan Perluasan Tanaman Lada. Direktorat Jenderal Perkebunan. Kementerian Pertanian RI, Jakarta. 45 p.
Arif, M., D. R. Pani, N. W. Zaidi, & U.S.Singh. 2011. PCR-based Identification and Characterization of Fusarium sp. Associated with Malformation. Biotechnology Research International 2011: 1–6.
Booth, C. 1971. The Genus Fusarium. Commonwealth Mycological Institute Kew, Surrey, England. 237 p.
Carnaúba, J.P., M.F. Sobral, E.P. da Rocha Amorim, & I.O. Silva. 2007. Report of Fusarium solani f.sp. piperis in Piper nigrum in the State of Alagoas. Summa Phytopatologica 33: 96–97.
Duarte, M.L.R. & S.A. Archer. 2003. In vitro Toxin Production by Fusarium solani f. sp. piperis. Fitopatologia Brasileira 28: 229–235.
Eisenback J.D. 1985. Diagnostic Characters Useful in the Identification of the Four Most Common Species of Root-knot Nematodes (Meloidogyne spp.), p. 95−112. In J.N. Sasser & C.C. Carter (eds.), An Advanced Treatise on Meloidogyne. Vol. I. Biology and Control. North Carolina State University, Raleigh, North Carolina.
Eisenback, D., H. Hirschmann, J.N. Sasser, & A.C. Triantaphyllou.1981.A Guide to the Four Most Common Species of Root-Knot Nematodes (Meloidogyne spp.), with a Pictorial Key. The Department of Plant Pathology and Genetics North Carolina State University and The United States Agency for International Development Raleigh, North Carolina. 52 p.
Hamada, M., T. Uchida, & M. Tsuda. 1988.Ascospore Dispersion of the Causal Agent of Nectria Blight of Piper nigrum. Annals of the Phytopathological Society of Japan 54: 303–308.
Hooper, D.J., 1985. Extraction of Free-living Stages from Soil, p. 5–30. In Southey, J.F. (ed.), Laboratory Methods for Work with Plant and Soil Nematodes. Ministry of Agriculture, Fisheries and Food, London.
Hooper, J.H., J. Hallmann & S.A. Subbotin. 2005. Methods for Extraction, Processing and Detection of Plant and Soil Nematodes, p 53− 6. In M. Luc, R.A. Sikora & J. Bridge. (eds.), Plant Parasitic Nematodes in Subtropical and Tropical Agriculture. 2nd ed. CABI Publ., Wageningen, Netherland.
Hunt, D.J., M. Luc, & R.H. Manzanilla-López. 2005. Identification, Morphology and Biology of Plant Parasitic Nematodes, p. 11–52. In M. Luc, R.A. Sikora, & J. Bridge (eds.), Plant Parasitic Nematodes in Subtropical and Tropical Agriculture. 2nd ed. CABI Publ., Wallingford, Oxfordshire.
Koshy, P.K., S.J. Eapen & R. Pandey. 2005. Nematode Parasites of Spices, Condiments and Medicinal Plants, p. 751−792. In M. Luc, R.A. Sikora & J. Bridge. (eds.), Plant Parasitic Nematodes in Subtropical and Tropical Agriculture. 2nd ed. CABI Publ., Wageningen, Netherland.
Leslie, J.F. & Summerell, B.A. 2006. The Fusarium Laboratory Manual. Blackwell Publishing, Victoria. 388 p.
Manohara, D. & Nurheru. 2002. Hama dan Penyakit Utama Tanaman Lada dan Pengendaliannya. Warta Penelitian dan Pengembangan Pertanian 29: 5–6.
Manohara, D. & D. Wahyuno. 2009. Kontroversi Penggunaan Bungkil Jarak (Ricinus communis) pada Penyakit Busuk Pangkal Batang dan Penyakit Kuning Tanaman Lada. Warta Penelitian dan Pengembangan Tanaman Industri 15: 1–3.
Mustika, I. 1990. Studies on the Interactions of Meloidogyne incognita, Radopholus similis and Fusarium solani on Black Pepper (Piper nigrum L.) Disertation. Landbouwuniversitet, Wageningen. Unpublish. 127 p.
Nugroho, S.E. 1990. Penyakit Kuning dan Kerdil Tanaman Lada dan Pengendaliannya. Berita Perlindungan Tanaman Perkebunan 2: 6–7.
Sahoo, N. K., S. Ganguly, S.J. Eapen. 2000. Description of Meloidogyne piperi sp.n. (Nematoda: Meloidogynidae) Isolated from the Roots of Piper nigrum in South India. Indian Journal of Nematology 30: 203−209.
Singleton, L.L., J.D. Mihail, & C.M. Rush. 1992. Methods for Research on Soil Borne Phytopathogenic Fungi. APS. Press, St. Paul Minnesota. 265 p.
Thuy, T.T.T. 2010. Incidence and Effect of Meloidogyne incognita (Nematoda: Meloidogyninae) on Black Pepper Plants in Vietnam. Thesis. Katholieke Universiteit Leuven, België. Unpublish. 138 p.
Vaz,A. B., V. G. Elizei, S. S. Costa, & L. H. Pfenning. 2012. First Report of Sexual Reproduction of Fusarium solani f. sp. piperis in Bahia, Brazil. Plant Disease 96: 1581–1581.
DOI: https://doi.org/10.22146/jpti.16019
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