The Role of Extracellular Protein on the Pathogenicity of Xanthomonas campestris pv. citri

https://doi.org/10.22146/jpti.12405

Tri Joko(1*), Siti Subandiyah(2), Susamto Somowiyarjo(3)

(1) Fakultas Pertanian Universitas Gadjah Mada
(2) Fakultas Pertanian Universitas Gadjah Mada
(3) Fakultas Pertanian Universitas Gadjah Mada
(*) Corresponding Author

Abstract


A research on the pathogenicity of Xanthomonas campestris pv. citri, the causal agent of citrus canker, has been carried out to study the growth characteristics of the pathogen on some media, physiological characteristics, and the role of extracellular protein on the bacterial pathogenicity. Extracellular protein of X. campestris pv. citri was extracted using ammonium sulfate precipitation. The extracted protein samples were electrophoresed on 10% polyacrilamide gel in the presence of sodium dodecyl sulfate at 15 mA/150 V for 1.5-2 hrs. Pathogenicity assay was conducted by infiltration of bacterial cell and extracellular protein suspension into citrus leaf tissues. The results showed that X. campestris pv. citri was able to grow well on all media. It possess specific protein with molecular weight of 25.71 KDa. Bacterial cell and extracellular protein of X. campestris pv. citri were able to produce typical symptoms of canker, while other closely related Xanthomonas campestris pathovars were only able to produce hypersensitive reaction on citrus leaves.


Keywords


Xanthomonas campestris pv. citri; extracellular protein; pathogenicity

Full Text:

PDF


References

Ausubel, F.M., R. Brendt, R.E. Kingston, D.D. Moore, J.G. Seidmen, J.A. Srruth & K. Struhl. 1989. Current Protocol in Molecular Biology. John Willey, New York.

Daniels, M.J. 1989. Pathogenicity of Xanthomonas and Related Bacteria towards Plants, p. 353-369. In Hopwood, P.A. & K.F. Chater (eds.), Genetics of Bacterial Diversity. Acad. Press, San Diego.

De Boer, S.H. & N.W. Schaad. 1990. Preparation of Ant~ens Bacteria, p. 27-30. In Hampton, R., E. Ball & S. De Boer (eds.), Serological Methods for Detection and Identification of Viral and Bacterial Plant Pathogen. APS Press, Minnesota.

Fahy, P.C. & A.C. Hayward. 1983. Media and Methods for Isolation and Diagnostic Tests, p. 337-378. In Fahy, F.C. & G.J. Presley (Eds.), Plant Bacterial Diseases, A Diagnostic Guide. Acad. Press, Sydney.

Flor, H.H. 1942. Inheritance of Pathogenicity of Melampsora lini. Phytopathology 32: 653-669.

Gail, L.S. 1991. Plant Diseases, their Biology and Social Impact. Acad. Press, USA. 397 p.

Goto, M. 1992. Fundamentals of Bacterial Plant Pathology. Acad. Press, Tokyo. 342 p.

Hahn, M.G. 1996. Microbial Elicitors and their Receptors in Plants. Ann. Rev. Phytopathol. 34: 387-412.

Hartung, J.S. & E.L. Civerolo. 1989. Restriction Fragment Length Polymorphism Distinguished Xanthomonas campestris Strain Isolated from Florida Citrus Nurseries from X.c. pv. citri. Phytopathology 79: 793-799.

He, S.Y., M. Lindberg & A. Collmer. 1993. Protein Secretion by Plant Pathogenic Bacteria, p. 39-64. In Chet, I. (ed.), Biotechnology in Plant Diseases Control. John Willey, New York.

Hugouvieux, V., C.E. Barber & M.J. Daniels. 1998. Entry of Xanthomonas campestris pv. campestris into Hydathodes of Arabidopsis thaliana Leaves: A System for Studying Early Infection Events in Bacterial Pathogenesis. Mol. Plant-Microbe Interact. 11 (6): 537-543.

Moffett, M.J. & B.J. Croft. 1983. Xanthomonas, p. 189-222. In Fahy, P.C. & G.J. Presley (eds.), Plant Bacterial Diseases, A Diagnostic Guide. Acad. Press, Sidney.

Mukherjee, A., Y. Cui & A.K. Chatterjee. 1997. Molecular Characterization and Expression of the Erwinia carotovora hrpNEcc Gene, which Encodes an Elicitor of the Hypersensitive Reaction. Mol. Plant-Microbe Interact. 10 (4): 462-471.

Mulyadi. 1990. Analisis Makromolekul. PAU Bioteknologi UGM, Yogyakarta. 183 p.

Nomura, K., W. Nasser, H. Kawagishi & S. Tsuyumu. 1998. The pir Gene of Erwinia chrysanthemi EC16 Regulates Hyperinduces of Pectate Lyase Virulence Genes in Response to Plant Signals. Proc. Natl. Acad. Sci., USA. 95: 14034-14039.

Semangun, H. 1994. Penyakit-penyakit Tanaman Hortikultura di Indonesia. Gadjah Mada Univ. Press, Yogyakarta. 850 hlm.

Singh, K.G. 1980. A Checklist of Host and Disease in Malaysia. Min. Agric., Malaysia. 280 p.

Vanderplank, J.E. 1978. Genetic and Molecular Basis of Plant Pathogenesis. Springer-Verlag, Berlin.

Vanderplank, J.E. 1982. Host-Pathogen Interactions in Plant Disease. Acad. Press, New York. 207 p.

Whiteside, J.D., S.M. Garnsey & L.W. Timer. 1989. Compendium of Citrus Diseases. APS Press, USA. 80 p.



DOI: https://doi.org/10.22146/jpti.12405

Article Metrics

Abstract views : 1392 | views : 897

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2000 Jurnal Perlindungan Tanaman Indonesia

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print), ISSN 2548-4788 (online) is published by the Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.  

View website statistics