Seleksi, Karakterisasi, dan Reaktivitas Antibodi Monoklonal Virus Kerdil Kedelai

https://doi.org/10.22146/jpti.12233

Sri Sulandari(1*), Y. B. Sumardiyono(2), Roechan M.(3)

(1) Fakultas Pertanian Universitas Gadjah Mada
(2) Fakultas Pertanian Universitas Gadjah Mada
(3) Pusat Penelitian Bioteknologi Tanaman Pangan Bogor
(*) Corresponding Author

Abstract


Soybean Stunt Virus (SSV) is a member of Cucumber mosaic virus group that caused soybean stunt disease. The disease is the most important viral disease of soybean in Indonesia. The objective of the study was to obtain SSV monoclonal antibody for detection SSV in both infected seed and plant. Six hybridoma clones were obtained from fusion between spleen cells of BALB/c immunized with Soybean stunt virus and mouse myelome cell lines (NS-1); they were IgG3 type antibodies, and its titres were varied between 1: 1O to 1: 1OO. Using non-recoated I-ELISA method, the homolog antigen (SSV) was well detected but not the heterolog antigens (CMV isolated from banana, tobacco).

Keywords


Soybean Stunt Virus; monoclonal antibody; selection

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References

Artama, W.T. 1990. Antibodi Monoklonal, Teori, Produksi, Karakterisasi, dan Penerapan. Pusat Antar Universitas Bioteknologi, UGM, Yogyakarta. 194p.

Chen, J., L. Torrance, G.H. Cowan, S.A. Mac Farlane, G. Stubbs, & Wilson, T.M.A. 1997. Monoclonal Antibodies Detect a Single Amino Acid Difference Betwen the Coat Proteins of Soilborne Wheat Mosaic Virus Isolates: Implications for Virus Structure. Phytopathology 87: 295- 301.

Koenig, R. 1991. Indirect-Elisa Methods for the Broad Specifity Detection of Plant Viruses. J. Gen. Virol. 55: 53-62.

Lange, L. 1985. Seed Transmitted Virus Diseases. Biology, Detection, and Control. Danish Government Inst. of Seed Pathology for Developing Country.

Lankow, R.K., S.H. Woodhead, R.J. Patterson, R. Massey, & G. Schohetman.1984. Monoclonal Antibody Diagnostics in Plant Disease Management. Plant Disease 68: 1100-1101.

Mahmood, T., Hein, G.L. & French, R.C. 1997. Development of Seroogical Procedures for Rapid and Reliable Detection of Wheat Streak Mosaic Virus in Single Wheat Curl Mite. Plant Disease 81: 250- 253.

Matthews, R.E.F. 1992. Fundamental of Plant Virology. Academic Press.

Memaugh, R.L., G.R. Memaugh, & G.R. Kovacs. 1990. The Immune Response: Antigen, Antibodies, Antigen-Antibody Interaction. Dalam: Hampton, R., E. Ball. S. De Boer (Eds.). Serological Methods for Detection and Identification of Viral and Bacterial Plant Pathogens. APS Press., St. Paul.Minnesota, USA. p: 3-26.

Nikolaeva, O.V., Karasev, A.V., Powell, C.A., Gumpf, D.J., Garnsey, S.M., & Lee, R.F. 1996. Mapping of Epitopes for Citrus Tnsteza Virus Spesific Monoclonal Antibodies Using Bacterially Expressed Coat Protein Coat Protein Fragments. Phytopathology 86: 974 - 979.

Qadri, F., Mohi, M.G., Chowdhury, A., Alam, K., Azim, T., Sears, c., Sack, R.B., & Albert, M.J. 1996. Monoclonal Antibodies to the Anterotoxin of Bacteroides fragilis: Production, Characterization, and Immunodiagnostic Application. American Soc.for Microbiology. 3: 608-610.

Roechan, M. 1992. Penyakit-Penyakit Virus pada Tanaman Kedelai. Disertasi S3. Univ. Padjajaran, Bandung. (tidak dipublikasikan).

Sudjono, M. Amir, & R. Martoatmodjo. 1985. Penyakit Kedelai dan Penanggulangannya. Dalam: Somaatmadja, S. (Ed.). Kedelai. Balai

Sulandari, S, Y.B. Sumardiyono, & M. Roechan. 1998. Reaktivitas Antibodi Poliklonal SSV terhadap Antigen yang Homolog dan Heterolog. J. Perlind. Tan. lndon. 4: 51 - 56.

Somowiyarjo, S., N. Sako, & F. Nonaka. 1988. Production and Characterization of Monoclonal Antibodies to Watermelon Mosaic Virus 2. Ann. Phytopath. Soc. Japan 56: 541-548.

Somowiyarjo, S. 1991. Seleksi, Karakterisasi, Produksi, dan Purifikasi Antibodi Monoclonal. Laboratorium Virologi, Fakultas Pertanian UGM, Yogyakarta.

Su, H.J. & R.Y. Wu. 1989. Characterization and Monoclonal Antibodies of Virus Causing Banana Bunchy Top. Technical Bulletin No. 115, National Taiwan University, Taiwan, RRC.

Suastika, G., K. Tomaru, J. Kurihara, & K.T. Natsuaki. 1996. A Cucumber Mosaic Cucumovirus Isolate from Banana Mosaic Disease in Indonesia. Biological Control in Sustainable Tropical Agriculture. Nodai Center for International Programs, Tokyo University of Agriculture. JSPS-DGHE Program p: 60 - 71.

Suseno R. 1987. Virus Terbawa Benih Tanaman Pangan serta Hubungannya dengan Sertifikasi dan Pengawasan Mutu Benih. Lokakarya Patogen Terbawa Benih dalam Rangka Pengembangan Pengawasan Mutu dan Sertifikasi Benih, Bogor, 8 9 Desember 1987.

Tamada, T., T. Goto., I. Chiba, & T. Suwa. 1970. Soybean Dwarf, a New Virus Diseases. Ann. Phytopath. Soc. Japan 3 5: 282 - 285.

Thomas, J.E., Geering, A.D.W., Gambley, C.F., Kessling, A.F., & White, M. 1997. Purification, Properties, and Diagnosis of Banana Bract Mosaic Potyvirus and Its Distinction from Abaca Mosaic Potyvirus. Phytopahology 87: 123 - 131.

Torrance, L. 1992. Serological Methods to Detect Plant Viruses: Production and Use of Monoclonal Antibodies. Dalam Duncan, J. M. & L. Torrance (Eds.). Technique for the Rapid Detection of Plant Pathogens. Blackwell Scientific Publ.

Van Regenmortel, M.H.V. 1981. Serology of Plant Viruses. Acad. Press., New York.

Wahyuni, W.S., RG. Dietzgen, K. Hanada & R.I.B. Francki. 1992. Serological and Biological Variation Between and Within Subgroup I and II Strains of Cucumber Mosaic Virus. Plant Pathol. 41: 282- 297.



DOI: https://doi.org/10.22146/jpti.12233

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