Paraquat Toxicity on Root Nodule Formation on Macroptiliuma tropurpureum Urb. and Its Corelation with Population of Rhizobium sp.

https://doi.org/10.22146/jpti.12119

Erni Martani(1*), Sebastian Margino(2), Medhina Magdalena(3)

(1) Fakultas Pertanian Universitas Gadjah Mada
(2) Fakultas Pertanian Universitas Gadjah Mada
(3) Fakultas Pertanian Universitas Gadjah Mada
(*) Corresponding Author

Abstract


This study was designed to investigate the paraquat toxicity toward root nodulation by Rhizobium on Macroptilium atropurpureum as an indicator plant. The legume was grown in Thornton medium treated with several concentrations of paraquat and inoculated with R.japonicum 143 (Rj-143) or Rhizobium sp. C-1.1. These bacteria represent cross-inoculation of soybean and cover-crops legumes, respectively. Nodule formation and Rhizobium population were measured periodically. At the end of planting time, nitrogenase activity of the nodules was analysis based on ARA (Acethylene Reduction Analysis) method. The results showed that nodules in plants inoculated with Rhizobium without addition paraquat, were formed within four weeks. There was no nodulation when paraquat was added. Paraquat was toxic to the plant, causing chlorosis, stunting, drying of the plant tissues, and death. The symptoms were detected at the second week after planting time. Paraquat also decreased Rhizobium population from 10^6 to 10^2 or 10^1 CFU/mL at 40 and 100 pp, respectively. These results depicted that paraquat disturbed the plant before nodulation, and at the same time Rhizobium populatin decreased until below minimal population required for nodulation. Therefore, the process of nodulation was disturbed, and in some treatments there was nodulation. It was concluded that paraquat was toxic to both plant and the Rhizobium, which cause nodulation failure.

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References

Allen, O.N. & E.K. Allen, 1981. The leguminosae : A source book of characteristics, uses & nodulation. The University of Wisconsin Press. Wisconsin.

Alexander, M. 1993. Biodegradation & Bioremediation. John Wiley & Sons. London.

Anderson, J.R. 1978: Influence of pesticides on nontarget soil microorganisms. In HiH, J.R. & S.J.L. Wright. (Eds). Pesticide microbiology : Microbiological aspects of pesticide behaviour in the environment. Academic Press: London. P. 313 - 534.

Carr,R.J.G., R.F. Bilton & T. Atkinson. 1985. Mechanisms of Biodegradation of paraquat by Lipomyces starkeyi. Appl.Environ.Microbio. 49 : 1290 - 1294.

Graham, P.H. & J. Halliday. 1977. Inoculation and nitrogen fixation in the genus Pheseolus. In Vincent, J.M., AS. Whitney & J. Bose (Eds). Exploiting the legume Rhizobium symbiosis in tropical agriculture. College of Tropical Agriculture Miscellaneous Publication, Hawaii.

Hassan, H.M. & I. Fridovich. 1978. Superoxide radical & the oxygen enhancement of the toxicity of paraquat in Escherichia coli. J. Biol. chem. 253: 8143-8148.

Karban, R. & J. Kue. 1999. Induced resistence againts pathogen & herbivores : An overview. In Agrawal, A.A., S.Tuzun & E.Bent (Eds). Induced Plant defences againts pathogen and herbivores: Biochemistry, Ecology & Agriculture. P. 1 - 16.

Katayama, A. & S. Kuwatsuka. 1992. Microflora in Soils with long-term application of paraquat. J.Pesticide Sci. 17: 137 - 139.

Hammerschmidt, R. & J. A. Smith-Becker. 1999. The role of salicilic acid in disease resistance. In Agrawal, A.A., S.Tuzun & E.Bertt (Eds). Induced Plant defences againts pathogen & herbivores: Biochemistry, Ecology &Agriculture. P. 37-53.

Margino, S., E. Martani & B.H. Sunarminto. 2000. Paraquat herbicide in peat soil : I. Its Effects on the dynamics of microbial population. J. Perlindungan Tanaman Indonesia. 6: 91- 100.

Martani, E., BH Sunarminto, S. Margino, & S. Handayani. 2000. Herbisida paraquat dalam lahan gambut: Pengaruhnya Terhadap Tanah & Pertumbuhan Jagung. Jurnal Manusia & Lingkungan 7 : 35 - 44.

Martani, E, B.H. Sunarminto, A. Supriyo, & S. Margino. 2001. Herbisida Parakuat dalam Lahan Gambut: II. Pengaruhnya Terhadap Pertumbuhan & Hasil Kedelai. J. Perlindungan Tanaman Indonesia Vol. 7, No. 1. p. 22 - 31.

Martani, E., B.H. Sunarminto, S. Handayani & A. Supriyo. 2002. Pertumbuhan tanaman padi pada bahan gambut yang diperlakukan dengan herbisida paraquat, kapur & pupuk NPK: Gama Sains; 4 : 7 - 15.

Martani, E. 2002. Paraquat toxicity on the growth of Rhizobium sp. in a synthetic medium. J Perlindungan Tanaman Indonesia. 8: 124- 131.

Pasaribu, D., N. Sumarlin, Sumarno, Y. Supriati, R. Saraswati, P. Sutjipto & S. Karama. 1989. Penelitian inokulasi Rhizobium di Indonesia. In M. Syam, Ruhendi & A. Widjono (Eds.). Penelitian penambatan nitrogen secara hayati pada kacang-kacangan. BP3 Jakarta. P. 3 -29.

Rao., N.S.S. 1994. Mikroorganisme Tanah & Pertumbuhan Tanaman. 2nd ed. UI Press. Jakarta. 76 pp.

Roughly, R.J. & D.J. Pulsford. 1982. Production and Control of Legume Inoculant. p. 193-209. In Vincent, J.J. (Ed) Nitrogen fixation in legumes. Academic Press. Sydney.

Setyaningsih, R., E. Martani & BH. Sunarminto. 2001. Dinamika Populasi Mikroorganisme yang Berperan Dalam Nitrifikasi di Beberapa Jenis Tanah Akibat Perlakuan Paraquat. BioSMART. Vol. 3, No 1,7-13.

Soejono, A.T. 1986. Penggunaan herbisida secara pra-tanam pada kacang tanah. Laporan Penelitian. Proyek PPPT-UGM 1985/1986. Fak. Pertanian UGM. Yogyakarta.

Vidor, C. 1981. Microbial constraints to legume symbiosis. In Graham, P.H. & S.C. Harris (Eds). Biological nitrogen fixation technology for tropical agriculture. Centro International de Agricultura Tropical. Columbia. P. 27 -35.

Vincent, J.M. 1982. Nature & basic properties of the Rhizobia. In Nitrogen fixation in Legumes. Vincent J.J. (Ed). Academic Press. Sydney. P. 5 - 11.



DOI: https://doi.org/10.22146/jpti.12119

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