Colonization of Tomato Root by Antagonistic Bacterial Strains to Fusarium Wilt of Tomato

Arif Wibowo(1*)

(1) Fakultas Pertanian Universitas Gadjah Mada
(*) Corresponding Author


Fusarium wilt of tomato caused by Fusarium oxysporum f.sp. lycopersici (Fol) is an important disease in tomato which cause a significant loss of yield in major growing regions of the world. This study examined the ability of bacterial strains antagonistic to F. oxysporum f.sp. lycopersici (H5, H22, H63, H71, Burkholderia cepacia strain 65 and 526) to colonize tomato seedlings and the effect of plant growth. The effect of bacterial population size and air temperature on the bacterial colonization and their spread along the root systems was also assessed.The results of this study showed that the bacterial population at 28°/23° C day/night temperature 14 days after planting was significantly greater than 23°/18° C for 4 of 6 strains tested. Although there was no significant effect of temperature on bacterial population observed in this study, the ability of the baacterial strains to colonize the rhizosphere was significantly different. Three strains (H5, B. cepacia strain 65 and 526) survived well in the rhizosphere and at 4 weeks after planting rhizosphere populations per gram fresh root were not significantly different from those recovered 2 weeks after planting. The largest population of the bacterial inoculants developed in the basal region of the roots and this differed between strains by log10 2.7 cfu/cm root. The bacterial populations in other parts of the root were also strain dependent. Strain H71, for example, was able to colonize the root segments at a high population level. However strain H63 was recovered only in small number in all root segments.


colonization of tomato root; bacterial strain antagonistic; Fusarium wilt tomato

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Bennet, R.A., & J.M. Lynch. 1981. Bacterial growth and development in the rhizosphere of gnotobiotic cereal plants. Journal of general Microbiology. 125: 95-102.

Brown, M.E. 1974. Seed and root bacterization. Annual Review of Phytophatology. 12: 181-197.

Bull, C.T., D.M. Weller,& L.S. Thomashow. 1991. Relationship between root colonization and suppression of Gaeumannomyces graminis var. tritici by Pseudomonasfluorescent strain 2-79. Phytopathology 81: 954-959.

Chao, W., R. Li, & W. Chang. 1988. Effect of root agglutinin on microbial activities in the rhizosphere. Applied and Environmental Microbiology. 54: 1838-1841.

Curl. E.A.,& B. Truelove. 1986. The rhizosphere. Springer Verlag. Berlin. Pp. 289.

De Freitas, J .R., & J .J. Germida. 1992. Growth promotion of winter wheat by fluorescent psedomonads under growth chamber condition. Soil Biology and Biochemistry. 24: 1127-1135.

Glandorf, D.C.M., I. Brand, P.A.H.M. Baker,& B. Schipper. 1992. Stability of rifampicin resistance as a marker for root colonization studies of Pseudomonas putida in the field. Plant and soil 147: 135-142.

Glandorf, D.C.M., I van der Sluis, A.J. Anderson, P.A.H.M. Baker,& B. Schipper. 1994. Agglutination adherence and root colonization by fluorescent pseudomonads. Applied and Environmental Microbiology. 60: 1726-1733.

Gratidge, R., & R.G. O'Brien. 1982. Occurrence of a third race of Fusarium wilt of tomatoes in Queensland. Plant Disease 66: 165- 166.

Hebbar, K.P., A.G Davey, J. Merrin, & P.J. Dart. 1992a. Rhizobacteria of maize antagonistic to Fusarium moniliforme, a soil borne fungal pathogen: colonization of rhizosphere and roots. Soil Biology and Biochemistry. 24: 989-997.

Hebbar, K.P., A.G Davey, J. Merrin, T.J. McLoughlin, & P.J. Dart. 1992b. Rhizobacteria of maize antagonistic to Fusarium moniliforme, a soil-borne fungal pathogen: colonization of rhizosphere and roots. Soil Biology and Biochemistry. 24: 989-997.

Jjemba, P.K., & M. Alexander. 1999. Possible determinants of rhizosphere competence of bacteria. Soil Biology and Biochemistry 31: 623-632.

Jones, D.A., M.H. Ryder, B.G. Clare, S.K. Farrand, ~ ·A. Kerr. 1988. Construction Tradeletion mutant of pAgK84 to safeguard the biological control of crown gall. Molecular and General genetics 212: 207-214.

Lemanceau, P., & C. Alabouvette. 1991. Biological control of fusarium diseases by fluorescent Pseudomonas and non-pathogenic Fusarium. Crop Protection 10: 279-286.

Nautiyal, C.S. 1997. Rhizosphere competence of Pseudomonas sp. NBR19926 and Rhizobium sp. NBR19513 involved in the suppression of chickpea ( Cicer arieruinum L.) pathogenic fungi. FEMS Microbial Ecology 23: 145-158.

Parke, J.L. 1991. Root colonization by indigenous and introduced microorganisms. In: D.L. Keister and P.B. Cregan (eds.). The rhizosphere and plant growth. Kluwer Academic· Press. Dordrecht. Pp 33-42.

Seong, K.Y., M. Hofte, J. Boelens, & W. Verstraete. 1991. Growth, survival and root colonization of plant growth beneficial Pseudomonas fluorescens ANP15 and Pseudomonas aeruginosa 7NSK2 at different temperatures. Soil Biology and Biochemistry 23: 423-428.

Simons, M., A.J. van der Bij, I. Brand, L.A de Weger, A.C. Wijffelman, & B.J.J. Lugtenberg. 1996. Gnotobiotic system for studying rhizosphere colonization by plant growth promoting pseudomonas bacteria. Molecular plant-Microbe Interaction 9: 600-607.

Vesper, S.J. 1987. Production of pili (fimbriae) by Pseudomonas fluorescens and correlation with attachment to corn roots. Applied and Environmental Microbiology 53: 1397-1405.

Wibowo, A., P.J. Dart, & H.J. Ogle. 1999. Biological control of Fusarium wilt of tomato by antagonistic bacteria. Conference Handbook of 1211, APPS Biennial Conference. Canberra 27-30 September 1999. p. 183.


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