Observasi dan Identifikasi Virus yang Menginfeksi Bawang Merah di Jawa

https://doi.org/10.22146/jpti.11883

Tuty Arisuryanti(1*), Budi Setiadi Daryono(2), Sedyo Hartono(3), Anak Agung Gde Raka Swastika(4)

(1) Fakultas Biologi Universitas Gadjah Mada
(2) Fakultas Biologi Universitas Gadjah Mada
(3) Fakultas Pertanian Universitas Gadjah Mada
(4) Pusat Kedokteran dan Kesehatan Markas Besar Kepolisian Republik Indonesia
(*) Corresponding Author

Abstract


This study was conducted to observe and identify viruses from infected shallots in several shallot planting center. The observation was done in eight areas of three provinces (Yogyakarta, Central Java, and East Java). Leaves from shallot plants and shallot germination showing virus symptoms were examined. The leaves were then investigated to identify viruses infecting shallots using Enzyme-Linked Immunosorbent Assay (ELISA). The result revealed that the type of virus symptoms infecting the shallots was a mozaic symptom with yellow strips. The ELISA analysis showed that Tawangmangu Biru shallot cultivar plants sampled from Blumbang, Tawangmangu (Central Java) and Philiphine Bima shallot cultivar seeds collected from Srigading, Sanden, Bantul (Yogyakarta) were positively infected by Onion Yellow Dwarf Virus (OYDV). The result also revealed that Biru, Kuning Tablet, Lokal Tawangmangu, and Bima Curut shallot cultivars had the potency to be virus resistant plants and could be considered as candidates for breeding program

 

Penelitian ini dilakukan untuk mengobservasi gejala serangan dan mengidentifikasi virus yang menginfeksi bawang merah di beberapa wilayah sentral budidaya bawang merah di Jawa. Observasi dilakukan pada delapan wilayah di tiga propinsi (DIY, Jawa Tengah, dan Jawa Timur). Tipe serangan virus diamati dari daun bawang merah dan bibit bawang merah yang memperlihatkan gejala terserang virus. Selanjutnya identifikasi isolat virus yang menyerang daun bawang merah dan bibit bawang merah dilakukan menggunakan teknik Enzyme-Linked Immunosorbent Assay (ELISA). Hasil penelitian menunjukkan bahwa tipe serangan virus baik pada daun bawang merah maupun bibit bawang merah adalah mozaik disertai strip berwarna kuning. Hasil ELISAmenunjukkan bahwa tanaman bawang merah kultivar Tawangmangu Biru dari Blumbang, Tawangmangu (Jawa Tengah) dan bibit bawang merah kultivar Philiphine Bima dari Srigading, Sanden, Bantul (DIY) positif terkena Onion Yellow Dwarf Virus (OYDV). Hasil penelitian juga memperlihatkan bahwa bawang merah kultivar Biru, Kuning Tablet, Lokal Tawangmangu, dan Bima Curut memiliki potensi tahan terhadap virus OYDV, sehingga ketiga kultivar bawang merah tersebut dapat dipertimbangkan sebagai kandidat untuk program pemuliaan.


Keywords


OYDV, shallots, virus symptoms; : bawang merah, gejala virus.

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References

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DOI: https://doi.org/10.22146/jpti.11883

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