Hubungan Antara Aktivitas Poligalakturonase Dengan Virulensi RAS 4 Fusarium oxysporum f.sp. cubense

https://doi.org/10.22146/jpti.11871

Arif Wibowo(1*), Siti Subandiyah(2), Christanti Sumardiyono(3)

(1) Fakultas Pertanian Universitas Gadjah Mada
(2) Fakultas Pertanian Universitas Gadjah Mada
(3) Fakultas Pertanian Universitas Gadjah Mada
(*) Corresponding Author

Abstract


One of the major constraints of banana plantation in Indonesia is the occurrence of fusarium wilt disease caused by Fusarium oxysporum f.sp. cubense. The pathogen produced series of cell wall degradation extracellular enzymes which have important roles in pathogenicity. Many studies have been conducted to know the role of degrading enzyme banana pectin is the major component of cell wall. Many pectinolytic enzymes such as polygalacturonase and others have been isolated from many fungal plant pathogens. The study was aimed to know the role of polygalacturonase towards the virulence of race 4 of F. oxysporum f.sp. cubense. The result showed that from 10 isolates of race 4 of F. oxysporum f.sp. cubense, the most virulent isolate was Lmp1 followed by Srg1, Bgl6, Mln1, Bgl3, A13, Bnt2, Gnk3, Kjg1 dan Wsb5. This was indicated by high and low percentage of wilting leaves of banana cultivar Cavendish when they were inoculated with these isolates. Incubation period varied from 3 to 6 weeks after inoculation SDS-PAGE showed that polygalacturonase, mostly PG1 and PG2, was secreted by these isolates, whereas PG3 was only found in growing cultures of Gnk3 and Wsb5 isolates. Detection of polygalacturonase activity with diffusion agar and reducing sugar methods showed that the activity of polygalacturonase secreted by F. oxysporum f.sp. cubense in the growing culture had no correlation with the virulence of the fungal pathogen.

 

Salah satu kendala utama dalam budidaya pisang di Indonesia adalah gangguan penyakit layu fusarium yang disebabkan oleh jamur Fusarium oxysporum f.sp. cubense. Jamur patogen ini menghasilkan serangkaian enzim ekstraselular pendegradasi dinding sel yang berperan dalam patogenesis. Beberapa penelitian telah dilakukan untuk mengetahui peran enzim yang mendegradasi pektin, yang merupakan komponen penting dinding sel tanaman. Beberapa enzim pektinolitik seperti poligalakturonase dan yang lainnya telah berhasil diisolasi dari berbagai spesies jamur penyebab penyakit tanaman. Penelitian ini bertujuan untuk mengetahui peranan poligalakturonase terhadap virulensi ras 4 F. oxysporum f.sp. cubense. Hasil penelitian menunjukkan bahwa dari 10 isolat F. oxysporum f.sp. cubense, yang mempunyai virulensi tertinggi hingga yang terendah berturut-turut adalah Lmp1, Srg1, Bgl6, Mln1, Bgl3, A13, Bnt2, Gnk3, Kjg1 dan Wsb5. Hal ini ditunjukkan dengan tinggi-rendahnya nilai persentase daun layu yang ditimbulkan oleh isolat-isolat tersebut pada bibit pisang kultivar Cavendish. Masa inkubasi bervariasi dari 3 hingga 6 minggu setelah inokulasi. Uji SDS-PAGE menunjukkan bahwa pada filtrat pertumbuhan isolat jamur-jamur patogen ini disekresikan poligalakturonase (PG) terutama PG1 dan PG2, sedangkan PG3 hanya dijumpai pada filtrat pertumbuhan isolat Gnk3 dan Wsb5. Adapun hasil deteksi aktivitas poligalakturonase dengan metode difusi agar serta gula reduksi menunjukkan bahwa aktivitas poligalakturonase yang disekresikan oleh F. oxysporum f.sp. cubense di dalam filtrat pertumbuhannya tidak berhubungan dengan virulensi jamur patogen tersebut.


Keywords


Fusarium oxysporum f.sp cubense; polygalacturonase; virulence; poligalakturonase; virulensi

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References

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DOI: https://doi.org/10.22146/jpti.11871

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