Hubungan Antara Aktivitas Poligalakturonase dengan Virulensi RAS 4 Fusarium oxysporum f.sp. cubense

Arif Wibowo(1*), Siti Subandiyah(2), Christanti Sumardiyono(3), Liliek Sulistyowati(4), Peter Taylor(5)

(1) Jurusan Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Gadjah Mada, Yogyakarta
(2) Jurusan Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Gadjah Mada, Yogyakarta
(3) Jurusan Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Gadjah Mada, Yogyakarta
(4) Fakultas Pertanian, Universitas Brawijaya, Malang
(5) School of Molecular and Microbial Sciences, The University of Queensland, St. Lucia
(*) Corresponding Author


One of the major constraints of banana plantation in Indonesia is the occurrence of fusarium wilt disease caused by Fusarium oxysporum f.sp. cubense. The pathogen produced series of cell wall degradation extracellular enzymes which have important roles in pathogenicity. Many studies have been conducted to know the role of degrading enzyme banana pectin is the major component of cell wall. Many pectinolytic enzymes such as polygalacturonase and others have been isolated from many fungal plant pathogens. The study was aimed to know the role of polygalacturonase towards the virulence of race 4 of F. oxysporum f.sp. cubense. The result showed that from 10 isolates of race 4 of F. oxysporum f.sp. cubense, the most virulent isolate was Lmp1 followed by Srg1, Bgl6, Mln1, Bgl3, A13, Bnt2, Gnk3, Kjg1 dan Wsb5. This was indicated by high and low percentage of wilting leaves of banana cultivar Cavendish when they were inoculated with these isolates. Incubation period varied from 3 to 6 weeks after inoculation SDS-PAGE showed that polygalacturonase, mostly PG1 and PG2, was secreted by these isolates, whereas PG3 was only found in growing cultures of Gnk3 and Wsb5 isolates. Detection of polygalacturonase activity with diffusion agar and reducing sugar methods showed that the activity of polygalacturonase secreted by F. oxysporum f.sp. cubense in the growing culture had no correlation with the virulence of the fungal pathogen.


Fusarium oxysporum f.sp cubense; polygalacturonase; virulence

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Agrios, G.N. 1988. Plant Pathology. Academic Press. San Diego, California. 803 p.

Asahi, T., M. Kajima., & T. Kosuge. 1979. The Energetics of Parasitism, Pathogenism, and Resistence in Plant Disease. Plant Disease 4 : 47-73.

Ausubel, F.M., R. Brant, R.E. Kinston, D.D.Moore, J.G. Seidman, J.A. Smith, & K. Struhl. 2002. Current Protocols in Molecular Biology. John Wiley and Sons. New York. 1600 p.

Baayen, R.P., E.A.M. Schoffelmeer, S. Toet, & D.M. Elgersma. 1997. Fungal Polygalacturonase Activity Reflects Susceptibility of Carnation Cultivars to Fusarium Wilt. European Journal of Plant Patholology 103: 15-23.

Bateman, D.F., & R.I, Millar. 1966. Pectic Enzymes in Tissue Degradation. Annual Review of Phytopathology 4: 119-146.

Beldman, G., L.A.M. van den Broek, H.A. Schols,M.J.F. Searle van Leuwen, K.M.J. van Laere, &A.G.J. Voragen. 1996. An Exogalacturonase from Aspergillus aculeatus Able to Degrade Xylogalacturonan. Biotechnology Letters 18: 707-712.

Biehn,W.L. & A.E. Diamond. 1953. Effect of Galactose on Polygalacturonase Production and Pathogenesis by Fusarium oxysporum f.sp. lycopersici. Phytopathology 61: 242-243.

Brown, J.F. & H.J. Ogle, 1997. Plant Pathogens and Plant Disease. Australian Plant Pathology Society. 556 p.

Chilosi, G. & P. Magro. 1997. Pectin Lyase and Polygalacturonase Isoenzymes Production by Botrytis cinerea during the Early Stages of Infection on Different Host Plants. Journal of Plant Pathology 78: 61-69.

Chilosi, G. & P. Magro. 1998. Pectolytic Enzymes Produced In Vitro and during Colonization of Melon Tissues by Didymella bryoniae. Plant Pathology 47: 700-705.

Collmer, A. & N.T. Keen. 1986. The Role of Pectic Enzymes in Plant Pathogenesis. Annual Review of Phytopathology 24: 383-409.

Di Pietro, A. & M.I.G. Roncero. 1996a. Endopolygalacturonase from Fusarium oxysporum f. sp. lycopersici: Purification, Characterization, and Production During Infection of Tomato Plant. Phytopathology 86: 1324-1330.

Di Pietro, A., & M.I.G. Roncero. 1996b. Purification and Characterization of an Exo-polygalacturonase from the Tomato Vascular Wilt Pathogen Fusarium oxysporum f.sp. lycopersici. FEMS Microbiology Letters 145: 295-299.

Di Pietro, A., F.I. Garcia-Maceira, M.D.Huertaz-Gonzalez, M.C. Ruiz Roldan, Z. Caracuel,A.S. Barbieri, & M. I.G. Roncero. 1998. Endopolygalacturonase PG1 in Different Formae Speciales of Fusarium oxysporum. Applied and Environmental Microbiology 64: 1967-1971.

Di Pietro, A. & M.I.G. Roncero. 1998. Cloning, Expression and Role in Pathogenicity of PG1 Encoding the Major Extracellular Endopolygalacturonase of the Vascular Wilt Pathogen Fusarium oxysporum. Molecular Plant-Microbe Interactions 11: 91-98.

Djatnika, I. & Nuryani, N. 1995. Pengendalian Biologi Penyakit Layu Fusarium pada Pisang dengan Beberapa Isolat Pseudomonas Fluorescens. p. 422-425. In Parman, A. Rohyadi, I.G.E. Gunartha, Murdan, L. Irasakti, M. Sarjan, & W. Wangiyana (eds.), Prosiding Kongres Nasional XIII dan Seminar Ilmiah PFI, Mataram 27-29 September. Universitas Mataram, Mataram.

Favaron, F., P. Alghisi, P. Marciano, & P. Magro. 1988. Polygalacturonase Isoenzymes and Oxalic Acid Produced by Sclerotinia sclerotiorum in Soybean Hypocotyls as Elicitors of Glyceollin. Physiological and Molecular Plant Pathology 33: 385-395.

Leone, G & J.V.D. Heuvel. 1987. Regulation of Carbohydrates of the Sequential in vitro Production of Pectic Enzymes by Botrytis cinerea. Canadian Journal of Botany 65: 2133-2141.

Lu, H., V.J. Higgins. 1993. Partial Characterization of a Non-proteinaceous Suppressor of Non-specific Elicitors from Cladosporium fulvum. Physiolological and Molecular Plant Pathology 42: 427-439.

Maceira, F.I.G., A.D. Pietro, & M.I.G. Roncero. 1997. Purification and Characterization of a Novel Exopolygalacturonase from Fusarium oxysporum f.sp. lycopersici. FEMS Microbiology Letters 154: 37-43.

Mendgen, K.,M. Hahn, & H. Deising. 1996.Morphogenesis and Mechanism of Penetration by Plant Phathogenic Fungi. Annual Review of Phytopathology 34: 367-386.

Oramahi, H.A. 2008. Penyakit Simpanan pada Gaplek yang Disebabkan oleh Aspergillus flavus. Disertasi Pascasarjana Universitas Gadjah Mada Yogyakarta (tidak dipublikasikan).

Pegg, K.G., N. Y. Moore, & S. Bentley. 1996. Fusarium Wilt of Banana inAustralia: a Review. Australian Journal of Agricultural Research 47: 637-650.

Ploetz, R.C. 1990. Population Biology of Fusarium oxysporum f.sp. cubense, p. 63-67. In R.C. Ploetz (ed.), FusariumWilt of Banana. APS Press, St. Paul Minnesota.

Ploetz, R.C. 2005. Panama Disease: An Old Nemesis Rears its Ugly Head Part 2. The Cavendish Era and Beyond. APSnet Feature Story. APS. St. Paul Minnesota.

Raymond J.T. & G.A. Secor. 1988.An Improved Diffuse Assay for Quantifying the Polygalacturonase Content of Erwinia Culture Filtrates. Department of Plant Pathology. North Dakota State University. Phytopathology 78: 1101-1103.

Riou, C., G. Freysinnet, & M. Fevre. 1992. Purification and Characterization of Extracellular Pectinolytic Enzymes Produced by Sclerotinia sclerotiorum. Applied and Environmental Microbiology 58: 578-583.

Su, H.J., S.C. Hwang, &W.H. Ko. 1986. FusarialWilt of Cavendish Bananas in Taiwan. Plant Disease 70: 814-818.

Vries, R.P. & J. Visser. 2001. Aspergillus Enzymes Involved in Degradation of Plant Cell Wall Polysaccharides. Microbiology and Molecular Biology Reviews 65:497-522.

Walton, D.J. 1994. Deconstructing the Cell Wall. Plant Physiology 104: 1113-1118.

Wibowo,A., Suryanti, & C. Sumardiyono. 2001. Patogenisitas 6 Isolat Fusarium oxysporum f.sp. cubense Penyebab Penyakit Layu Fusarium pada Pisang. Kongres XVI dan Seminar Nasional PFI, Bogor 22-24 Agustus 2001. Institut Pertanian Bogor. Bogor.

Wibowo, A., S. Subandiyah, C. Sumardiyono, L. Sulistyowati, P. Taylor, & M. Fegan. 2007. Diversity of Race 4 of Fusarium oxysporum f.sp. cubense Strains from Indonesia, p. 89-90. In Y.B. Sumardiyono, S. Hartono, Mulyadi, T. Arwiyanto, A. Widiastuti, T. Joko, & R. Kasiamdari (eds.), Proceedings the 3rd Asian Conference on Plant Pathology, Yogyakarta, Indonesia 20-24 August 2007. Faculty of Agriculture Gadjah Mada University, Yogyakarta.

Williams, P.H. 1979. How Fungi Induce Disease. Plant Disease 4: 163-179.


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