Deteksi Begomovirus pada Cabai Secara Cepat melalui Isolasi Genom DNA

Sri Sulandari(1*), Rusmilah Suseno(2), Sri Hendrastuti Hidayat(3), Soemartono Sosromarsono(4), Jumanto Harjosudarmo(5)

(1) Fakultas Pertanian Universitas Gadjah Mada
(2) Fakultas Pertanian Institut Pertanian Bogor
(3) Fakultas Pertanian Institut Pertanian Bogor
(4) Fakultas Pertanian Institut Pertanian Bogor
(5) Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik
(*) Corresponding Author


Pepper yellow leaf culr disease has been widely spreading in Indonesia, especially in Special Province of Yogyakarta and Central Java since 2000. The disease is difficult to control because its fast spreading over in the field by the vector. To prevent epidemic of the disease, early detection method of the causal agent is needed. The aim of the research was to detect the causal agent of the pepper yellow leaf curl disease by isolating the DNA genome. Using the Guanidine-alkaline method, two specific fragments of the DNA were produced approximately at 2600 bp and 1600 bp. The DNA fragments were similar with the DNA genome of Begomovirus. The method applied in this study is faster and easier for early detection of the Begomovirus in infected crop than detection by the Polymerase chain reaction (PCR).


Pepper yellow leaf curl disease; Begomovirus; Guanidine-alkaline method

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Bendahmane, M., H.J. Schalk, & B. Gronenbom, B. 1995. Identification and characterization of Wheat dwarf virus from France using rapid method for geminivirus DNA preparation. Phytopathol. 85: 1449-1455.

Brown, J.K. 1997. The Biology and Molecular Epidemiology of Geminivirus Subgroup III, p. 125- 195. In: Stacey, G& Keen, N.T. 1997: Plant-Microbe Interactions. Vol.2. International Thomson Publishing. New York.

Dijkstra, J., & C.P. de Jager. 1998. Practical Plant Virology: protocols and exercises. Springer lab manual. 459 p.

Fauquet, C.M., D.P. Maxwell, B. Gronenborn, & J. Stanley. 2000. Revised proposal for naming geminiviruses. Arch Virol 145/8: 1743-1761.

Gutierrez, C. 2000. Geminiviruses and the plant cell cycle. Plant Molecular Biology 43: 763-772.
Hamilton, W.D.O., R.C. Sanders, RH.A.Coutts, & K.W. Buck. 1981. Microbiology Letters 11: 263 - 267.

Hidayat, S.H., E.S. Rusli, & Nooraidawati. 1999. Penggunaan primer universal dalam Polymerase chain reaction untuk mendeteksi virus gemini pada cabe. Kongres Nasional XV dan Seminar Ilmiah PFI, Purwokerto, 16 - 18 September 1999.

Maniatis, T., E.F. Fritsch & J. Sanbrook. 1982. Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.

Mehta, P., J.A. Wyman, M.K. Naklila, & Maxwell, D.P. 1994. Polymerase chain reaction detection of viruliferuous Bemisia tabaci (Homoptera: Aleyrodidae) with two tomato infecting geminivirus. J. Econ. Entomol. 87 (5): 1285-1290.

Polston, J.E. &P.K.Anderson, 1997. The emergence of whitefly-transmitted geminiviruses in tomato in western Hemisphere. Plant Disease 81: 1358- 1369.

Rojas, M.R., R.L. Gilbertson, D.R. Russell, & D.P. Maxwell. 1997. Use of degenerate primers in the polymerase chain reaction to detect whitefly transmitted geminiviruses. Plant Disease 77: 340- 347.

Rosell, R.C, I. Torres-Jerez, & J.K. Brown. 1998. Tracing the geminiviruswhitefly transmission pathway by polymerase chain reaction in whitefly extract, saliva, hemolymph, and honeydew. Phytopathol 89:239-246.

Roye, M.E., W.A. McLaughlin, M.K. Nakhla. & D.P. Maxwell. 1997. Genetic diversity among geminiviruses associated with the weed species Sida sp., Macroptilium lathyroides, and Wissadula amplissima from Jamaica. Plant Dis 81: 1251- 1258.

Salati, R., M.K. Nahkla, M.R. Rojas P.Guzman, J. Jaquez, D.P. Douglas, & R.L. Gilbertson. 2002. Tomato yellow leaf curl virus in the Dominican Republic: Characterization of infectious clone, virus monitoring in whiteflies, and identification of reservoir hosts. Phytopathol 92:487- 496.

Samretwanich, K., P. Chiemsombat, K Kittipakom, & M. Ikegami. 2000. A new geminivirus associated with a yellow leaf curl disease of pepper in Thailand. Plan Dis 84: 1047.

Sulandari, S., S.H. Hidayat, R. Suseno H. Jumanto, & S. Sosromarsono.2001 Keberadaan virus gemini pada cabai di DIY. Kongres Nasional dan Seminar Ilmiah PFI ke XVI. Bogor, Agustus 2001.


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