Isolasi dan Karakterisasi Ralstonia syzygii

Yanuar Danaatmadja(1*), Siti Subandiyah(2), Tri Joko(3), Cavrina Ulita Sari(4)

(1) Program Studi Fitopatologi Pascasarjana Fakultas Pertanian Universitas Gadjah Mada
(2) Fakultas Pertanian Universitas Gadjah Mada
(3) Fakultas Pertanian Universitas Gadjah Mada
(4) PT Cengkeh Zanzibar
(*) Corresponding Author


Ralstonia syzygii is one of important pathogens of cloves. This study was aimed at obtaining pure culture of R. syzygi from diseased plant tissue samples. The acterium was isolated from infected clove plantations in Ungaran, Central Java. It had the ability to make clove seedlings show symptoms and then die 41 days after inoculation. The reisolated bacterium also showed its ability to kill clove seedlings after 17 days. The bacterium is gram negative, able to ultilize glucose, sucrose, maltose, mannitol, sorbitol, dulcitol, and glycerol, able to hydrolyze starch, grow well at 27oC, able to catalyze and release water and oxygen from hydrogen peroxyde (H2O2) and aerobically.


clove seedling; Ralstonia syzygii

Full Text:



Agrios, G.N. 2005. Plant Pathology 5th Edition. Elsevier Academic Press. London. UK. 922 p.

Anonim. 2009. Cengkeh., modified 19/4/2009.

Arlat, M., F. Van Gijsegum, J.C. Huet, J.C. Pernollet, & C.A. Boucher. 1994. PopA1, a Protein which Induces a Hypersensitive-like Response on Specific Petunia Genotypes, is Secreted via the Hrp Pathway of Pseudomonas solanacearum. EMBO Journal 13: 543–553.

De Baere, T., S. Steyaert, G. Wauters, P. De Vos, J. Goris, T. Coenye, T. Suyama, G. Verschraegen, & M. Vaneechoutte. 2001. Classification of Ralstonia pickettii biovar3/’thomasii’ Strains (Pickett 1994) and of New Isolates Related to Nosocomial Recurrent Meningitis as Ralstonia mannitolytica sp. nov. International Journal of Systematic EvolutionaryMicrobiology 51: 547–558.

Djafaruddin,A. Hanafiah, D. Suud, M. Syafruddin, & Mardinus. 1979. Penelitian Penyebab Utama Mati Massal (Mass Decline) pada Tanaman Cengkeh di Sumatera Barat. Fakultas Pertanian Universitas Andalas, Padang 23: 83–96.

Fahy, P.C., & G.J. Persley. 1983. Plant Bacterial Diseases; A Diagnostic Guide. Academic Press, Australia. 393 p. Fox, R.T.V. 1993. Principles of Diagnosis Techniques in Plant Pathology. CABInternational, UK. 213 p.

Guenoron M., A.C. Timmers, C. Boucher, & M. Arlat. 2000. Two Novel Proteins, PopB, which has Functional Nuclear Localization Signals, and PopC, which has a Large Luecin-rich Repeat Domain, are Secreted through the Hrp-secretion Apparatus of Ralstonia solanacearum. Molecular Microbiology 36: 261–277

Hadiwijaya, T. 1956. Penyakit Mati Bujang pada Cengkeh (Eugenia aromatica Baill.). Teknologi Pertanian 143

Lelliot, R.A., & D.E. Stead. 1987. Methods for the Diagnosis of Bacterial Diseases of Plants. Methods in Plant Pathology, British Society for Plant Pathology. Blackwell Scientific Publications. (2). 212 p.

Reitsma, J. 1953. Penyakit Cengkeh. Teknologi Pertanian 2: 404.

Roberts, S.J., S.J. Eden-Green, P. Jones, & D.J. Ambler. 1990. Pseudomonas syzygii sp. nov., the Cause of Sumatera Disease of Cloves. Systematic and Applied Microbiology 13: 34–43.

Schaad, N.W., J.B. Jones, & W. Chun. 2001. Laboratory Guide for Identification of Plant Pathogenic Bacteria 3rd edition. APS Press, Minnesota. 373 p.

Semangun, H. 2000. Penyakit-Penyakit Tanaman Perkebunan di Indonesia. Gadjah Mada University Press, Yogyakarta. 835 p.

Semangun, H. 2001. Pengantar Ilmu Penyakit Tumbuhan. Gadjah Mada University Press, Yogyakarta. 754 p.

Supriyadi. 1995. Karakteristik Pseudomonas solanacearum, P. syzygii dan Bakteri Penyebab Penyakit Darah (blood disease bacterium) pada Pisang. p. 557–581. Kongres Nasional XIII dan Seminar Ilmiah Perhimpunan Fitopatologi Indonesia.

Vaneechoutte, M., P. Kampfer, T. De Baere, E. Falsen, & G. Verscharaegen. 2004. Wautersia gen. nov., a Novel Genus Accomodating the Phylogenetic Lineage Including Ralstonia eutropha and Related Species, and Proposal of Ralstonia [Pseudomonas] syzygii (Roberts et al., 1990) comb. nov. International Journal of Systematic and Evolutionary Microbiology 54: 317–327.

Waller, J.M. & D. Sitepu. 1975. Sumatera Disease of Cloves in Indonesia. PANS 21, 141–147 Pembangunan Lembaga Penelitian Tanaman Industri 25: 58–70, 1977.


Article Metrics

Abstract views : 3933 | views : 2030


  • There are currently no refbacks.

Copyright (c) 2009 Jurnal Perlindungan Tanaman Indonesia

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print), ISSN 2548-4788 (online) is published by the Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.  

View website statistics