Identifikasi dan Virulensi Fusarium oxysporum f.sp. cubense RAS 4

https://doi.org/10.22146/jpti.11748

Dwi Kiswanti(1*), Suryanti Suryanti(2), Christanti Sumardiyono(3)

(1) Fakultas Pertanian Universitas Gadjah Mada
(2) Fakultas Pertanian Universitas Gadjah Mada
(3) Fakultas Pertanian Universitas Gadjah Mada
(*) Corresponding Author

Abstract


The aim of this study was to identify and to detect the virulence of Fusarium oxysporum f.sp. cubense (Foc) race 4. The isolates consisted of BNT-2, KD-1, U-8, BK, A-2, and A-13. Monospore isolation was done to obtain stable isolates. The detection and characteristics of isolates were observed on Komada medium. The diameter of colony and color was observed from underside of culture in petridish, while the shape of colony was observed from the upper side. Virulence test was conducted on Cavendish cultivar seedlings. Banana seedlings were inoculated with Foc cultured on rice medium (20 g/kg soil). The result indicated that A-13, U-8, BNT-2, and BK, were very virulent isolates; while A-2 and KD-1 were virulent. All isolates were detected as Foc race 4, with mild yellowish color and laccinated colonies on Komada medium.

 

Penelitian ini bertujuan mendeteksiras 4 dan virulensi Fusarium oxysporum f.sp. cubense (Foc).Isolat yang diuji adalah isolatBnt-2,KD-1,U-8,A-13,A-2, dan BK. Isolasi monospora dilakukan untuk memperoleh isolat yang stabil. Identifikasi dilakukan pada medium Komada. Pengamatan warna dan pengukuran diameter koloni dilakukan dari permukaan bawah cawan petri. Karakteristik morfologi yaitu bentuk koloni jamur diamati dari atas cawan petri. Uji virulensi dilakukan pada bibit pisang Cavendish. Bibit pisang Cavendish diinokulasi dengan Foc dalam medium beras sebanyak 20 g/kg tanah. Hasil pengamatan menunjukkan bahwa isolat A-13,U-8,BNT-2, dan BK sangat virulen. Isolat A-2 dan KD-1 virulen. Semua isolat diuji termasuk ras 4 dengan ciri-ciri warna koloni agak kekuningan dan tepi koloni bergerigi pada medium Komada.


Keywords


Foc race 4; Komada medium; Medium Komada; virulence; virulensi

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DOI: https://doi.org/10.22146/jpti.11748

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