Pengaruh Tinopal terhadap Patogenisitas Nucleopolyhedrovirus pada Spodoptera litura

https://doi.org/10.22146/jpti.11731

Ma'unah Ambarwati(1*), Arman Wijonarko(2), Sedyo Hartono(3)

(1) Program Pascasarjana Fakultas Pertanian Universitas Gadjah Mada
(2) Fakultas Pertanian Universitas Gadjah Mada
(3) Fakultas Pertanian Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Susceptibility of the armyworm, Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae) to nucleopolyhedrovirus was evaluated using droplet feeding methods. S. litura was originally collected from the field in Bantul and has been reared continuously in the laboratory using artificial diet. The tested instars were exposed a series concentration of nucleopolyhedrovirus (2×103, 2×104, 2×105, 2×106, 2×107, 2×108, 2×109 PIB/ml) which were added with Tinopal 0,5% and 1%. The result indicated that the larval mortality of 3rd, 4th, and 5th instars Tinopal, significantly different with the addition of 1% Tinopal. This addition increased the effectiveness of NPV for 235, 25117, and 6.6 million fold. The observation of the larval midgut which was treated by Tinopal, showed that Tinopal physically disrupt the peritrophic membrane. Therefore, it can be suggested that the Tinopal facilitates the entry of NPV to the host insect.

 

Kepekaan larva instar 3, 4, dan 5 ulat grayak, Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae), terhadap nucleopolyhedrovirus diuji dengan metode droplet feeding. Larva berasal dari lapangan yang dikembangbiakkan di laboratorium dengan pakan buatan. Larva S. litura yang diuji diperlakukan dengan berbagai konsentrasi SlNPV (2×103, 2×104, 2×105, 2×106, 2×107, 2×108, 2×109 PIB/ml) yang ditambahkan Tinopal (0,5% dan 1%). Hasil penelitian menunjukkan bahwa dengan penambahan Tinopal 1% berpengaruh signifikan terhadap kematian larva S. litura instar 3, 4, dan 5. Penambahan Tinopal pada larva instar 3, 4, dan 5 dapat meningkatkan efektivitas NPV sebesar 235, 24177, dan 6,6 juta kali. Pengamatan pada usus tengah larva yang diperlakukan dengan Tinopal, menunjukkan bahwa Tinopal secara fisik merusak membran peritropik sehingga diduga membantu masuknya virus ke dalam tubuh inangnya.

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Keywords


nucleopolyhedrovirus; pathogenicity; patogenisitas; S. litura; Tinopal

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DOI: https://doi.org/10.22146/jpti.11731

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