Identifikasi Ras Fisiologis Fusarium oxysporum f.sp. cubense Berdasarkan Sifat Kompatibel Secara Vegetatif dan Pembentukan Bahan Volatil

https://doi.org/10.22146/jpti.10099

Arif Wibowo(1*), Suryanti Suryanti(2), Christanti Sumardiyono(3)

(1) Fakultas Pertanian Universitas Gadjah Mada
(2) Fakultas Pertanian Universitas Gadjah Mada
(3) Fakultas Pertanian Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Race characterization of F. oxysporum Schlecht. f.sp. cubense (E.F. Smith) Snyd. & Hans. by determining disease reaction is difficult because the result may be biased due to the variability of growing condition. This study is aimed to identify physiological races of F. oxysporum f.sp. cubense in banana plantation in the province of Daerah Istimewa Yogyakarta by examining the relation of the fungal pathogen isolates. The identification of physiological races of F. oxysporum f.sp. cubense was based on vegetative compatibility groups (VCGs). The research was conducted in The Laboratory of Mycology Faculty of Agriculture GMU on March to November 2000. Observation of heterokarion formed by the mutant of F. oxysporum f.sp. cubense on selective medium was carried out in order to identify the compatibility of two different fungal isolates. Nitrate non utilizing (nit) mutants obtained without mutagen were used as the label. Nit mutant obtained from the same wild type isolates could form heterokarion on minimal agar medium containing NaNO3 as nitrogen source. Eleven isolates of F. oxysporum f.sp. cubense have been isolated from several cultivars of banana. Vegetative compatibility tests showed that of 11 eleven isolates, there were six different group VCGs where four of them formed volatile compound in rice medium whereas two of them did not.


Keywords


Fusarium wilt; F. oxysporum f.sp. cubense; vegetative compatibility groups (VCGs)

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References

Anonim. 1994. Petunjuk Teknis Peningkatan Mutu Pisang Hasil Kultur Jaringan. Direktorat Jendral Pertanian Tanaman Pangan. Jakarta.

Brake, V.M., K.G. Pegg, J.A.G. Irwin, & P.W. Langdon. 1990. Vegetative Compatibility Groups within Australian Populations of Fusarium oxysporum f.sp. cubense, the Cause of Fusarium Wilt of Bananas. Aus. J. of Agric. Research 41: 863─870.

Correll, J.C., J.E. Puhalla, & R.W. Schneider. 1987. Nitrate Nonutilizing Mutants of Fusarium oxysporum and their Use in Vegetative Compatibility Tests. Phytopathology77: 1640─1646.

Moore, N.Y., P.A. Hargreaves, K.G. Pegg, & J.A.G. Irwin. 1991. Characterization of Strains of Fusarium oxysporum f.sp. cubense by Production of Volatiles. Aus. J. of Botany 39: 161─166.

Pegg, K.G., R.G. Shivas, N.Y. Moore, & S. Bentley. 1995. Characterization of a Unique Population of Fusarium oxysporum f.sp. cubense Causing Fusarium Wilt in Cavendish Bananas at Carnarvon, Western Australia. Aus. J. of Agric. Research 46: 167─178.

Puhalla, J.E. 1985. Classification of Strain of Fusarium oxysporum on the Basis of Vegetative Compatibility. Canadian J. of Botany 63: 179─183.

Ploetz, R.C. 1993. Population Biology of Fusarium oxysporum f.sp. cubense, p 63─76. Dalam: R.C. Ploetz. Fusarium Wilt of Banana. APS Press. St. Paul.

Semangun, H. 1988. Penyakit-Penyakit Tanaman Hortikultura di indonesia. Gadjah Mada University Press.Yogyakarta. 850 p.

Su, EJ., H.J. Su, & W.H. Ko. 1978. Identification of Fusarium oxysporum f.sp. cubense Race 4 from Soil or Host Tissue by Cultural Characters. Phytopathology 68: 1672─1673.

Su, H.J., S.C. Hwang, & W.H. Ko. 1986. Fusarial Wilt of Cavendish Bananas in Taiwan. Plant Disease 70(9): 814─818.

Wibowo, A., Suryanti, & C. Sumardiyono. 2001. Patogenesitas 6 Isolat Fusarium oxysporum f.sp. cubense Penyebab Penyakit Layu Fusarium pada Pisang. Prosiding Kongres XVI dan Seminar Nasional Perhimpunan Fitopatologi Indonesia, Bogor 22─24 Agustus 2001. Bogor (dalam penerbitan).

Widyaningsih, S. 1998. Ketahanan Empat Kultivar Pisang Hasil Kultur Jaringan terhadap Penyakit Layu Fusarium. Sknpsi Fakultas Pertanian Universitas Gadjah Mada. Yogyakarta (tidak dipublikasikan).



DOI: https://doi.org/10.22146/jpti.10099

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