Genetic Relationship Between Colony Colour and Polyoxin Resistance in Cochliobolus heterostrophus

https://doi.org/10.22146/jpti.10075

Abdul Gafur(1*)

(1) College of Agriculture University of Lampung
(*) Corresponding Author

Abstract


Three reddish, polyoxin-resistant mutant strains of Cochliobolus heterostrophus (Drechsler) Drechsler (MIC > 1600 μg/ml), i.e. NA1, PRE058, and TE2, were isolated after ethyl methanesulphonate (EMS) mutagenesis. The resistant mutants were crossed to each other and to the greeny, sensitive wild type strain to investigate genetic relationship between colony colour and polyoxin resistance traits in the fungus. Progeny analyses showed that colony colour and polyoxin resistance in the three resistant mutants were conferred by monogenic genes. No linkage was detected in the present study between the genes controlling colony colour and polyoxin resistance traits in the fungus.


Keywords


Bipolaris maydis; Cochliobolus heterostrophus; colony colour; linkage; polyoxin resistance

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References

Becker, J.M., N.L. Covert, P. Shenbagamurthi, A.S. Steinfeld & F. Naider. 1983. Polyoxin D Inhibits Growth of Zoospathogenic Fungi. Antimicrob. Agents Chemoter. 23: 926─929.

Gafur, A. 1998. Generation of Fungicide-Resistant Mutant Strains of Cochliobolus Heterostrophus. Proceed. Indonesian Student Association Seminar, Nagaoka, Japan, March 7, 1998: 174─177.

Gafur, A. 1999. Identification of Minor Genes Controlling Polyoxin Resistance in Cochliobolus heterostrophus. Proceed. International Seminar on Toward Sustainable Agriculture in Humid Tropics Facing the 21st Century. Bandar Lampung, Indonesia, September 27─28, 1999: 395─401.

Gafur, A., C. Tanaka, K. Shimizu, S. Ouchi & M. Tsuda. 1998a. Molecular Analysis and Characterization of the Cochliobolus heterostrophus β-tubulin Gene and Its Possible Role in Conferring Resistance to benomyl. J. Gen. Appl. Microbiol. 44: 2 17─223.

Gafur, A., C. Tanaka, K. Shimizu, S. Ouchi & M. Tsuda. 1998b. Genetic Analysis of Cochliobolus heterostrophus polyoxin Resistant Mutants. Mycoscience 39: 155─159.

Gafur, A., C. Tanaka, S. Ouchi & M. Tsuda. 1997. A PCR-based Method for Mating Type determination in Cochliobolus heterostrophus. Mycoscience 38: 455─458.

Gafur, A., C. Tanaka, S. Ouchi & M. Tsuda. 2000. Studies on Iprodione Resistance in Cochliobolus heterostrophus. Proceed. 1st Asian Conference on Plant Pathology. Beijing, China, August 25─28, 2000. In press.

Georgopoulos, S.G. 1977. Development of Fungal Resistance to Fungicides, p. 439─495. In Sisler, H.D. & M.R. Siegel (eds.), Antifungal Compounds, vol. 2, Marcel Dekker, New York.

Isono, K., K. Asahi & S. Suzuki. 1969. Studies on Polyoxins, Antifungal Antibiotics. XXIII. The Structure of Polyoxins. J. Am. Chem. Soc. 91: 7490─7505.

Kiebacher, J. & G.M. Hoffman. 1981. Zur Genetik der Benzimidazol-Resistenz bei Venturia inaequalis. Z. Pflanzenkrankh. Pflanzenschutz 88: 189─205.

Martin, D., J.M. Oliver & Y. Lespinasse. 1981. Obtention In Vitro de Peritheces de Venturia inaequalis (Cke.) Wint.: Application a L’analyse de la Resistance au Benomyl Acquise au Verger. Agronomie 1: 745─749.

Shabi, E., T. Katan & K. Marton. 1983. Inheritance of Resistance to Benomyl in Isolates of Venturia inaequalis from Israel. Plant Pathol. 32: 207─211.

Shimizu, K., C. Tanaka & M. Tsuda. 1997. Cloning of Brn1, a Reductase Gene Involved in Melanin Biosynthesis in Cochliobolus heterostrophus. J. Gen. Appl. Microbiol. 43: 145─150.

Stanis, V.F. & A.L. Jones. 1984. Genetics of Benomyl Resistance in Venturia inaequalis from North and South America, Europe and New Zealand. Can. J. Plant Pathol. 6: 283─290.

Taga, M., H. Nagakubo, M. Tsuda & A. Ueyama. 1978. Ascospore Analysis of Kasugamycin Resistance in the Perfect Stage of Pyricularia oryzae. Phytopathology 68: 815─817.

Tanaka, C., Y. Kubo & M. Tsuda. 1991. Genetic Analysis and Characterization of Cochliobolus heterostrophus Color Mutants. Mycol. Res. 92: 49─56.

Tanaka, C., T. Taniguchi & M. Tsuda. 1994. Genetic Analysis of Cochliobolus heterostrophus Beige Mutants. Mycol. Res. 99: 621─622.

Tsuda, M. & A. Ueyama. 1987. Some Plant Pathogenic Fungi and Their Teleomorphs, p. 181─199. In Sugiyama, J. (ed.), Pleomorphic Fungi: The Diversity and Its Taxonomic Implications. Kodansha Ltd., Tokyo and Elsevier, Amsterdam.

Ueyama, A. & M. Tsuda. 1975. Formation in Culture of Cochliobolus miyabeanus, the Perfect State of Helminthosporium oryzae. Ann. Phytopathol. Soc. Japan 41: 434─440.



DOI: https://doi.org/10.22146/jpti.10075

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