Optimasi Produksi Endokitinase dari Jamur Mikoparasit Trichoderma reesei

https://doi.org/10.22146/jpti.10021

Harjono Harjono(1*), Siti Muslimah Widyastuti(2)

(1) Fakultas Kehutanan Universitas Gadjah Mada
(2) Fakultas Kehutanan Universitas Gadjah Mada
(*) Corresponding Author

Abstract


The objective of the experiment was to select optimal medium composition to induce endochitinase production of Trichoderma reesei. Growing media were prepared by substitution of 0.5% colloidal chitin, 0.5% colloidal chitin and 1% Polyvinyl Pyrrolidon (PVP), 1% crab shell chitin (Sigma), 1% crab shell chitin and 1% PVP into Richard’sMedium (RM). Selected bioagent T. reesei isolate 13 was grown onto the media and the specific activity of endochitinase was measured. The results showed that T. reesei grown in all media performed enzyme expression. The highest specific activity of endochitinase was detected on T. reesei grown in RM medium substituted with colloidal chitin and PVP, followed by the same fungi in RM medium substituted with crab shell chitin plus PVP, colloidal chitin and crab shell chitin alone.


Keywords


endochitinase; Trichoderma reesei; mycoparasite

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References

Danielson, R.M. & C.B. Davey. 1973. Non-nutritional Factors Affecting the Growth of Trichoderma in Culture. Soil Biol. Chem. 5: 495─504.

de La Cruz, J., J.A. Pintor-Toro, T. Benitez & A. Liobell. 1995. Purification and Characterization of an Endo-β-1,6- Glucanase from Trichoderma harztanum that is Related to its Mycoparasitism. J. Bacteriol. 177: 1864─1871.

de La Cruz, J., M. Rey, J.M. Lora, A. Hidalgo-Gallego, F. Dominguez, J.A. Pintor-Toro, A. Liobell & T. Benitez. 1993. Carbon Source Control on β-glucanase, Chitobiase and Chitinase from Trichoderma harzianum. Arch. Microbiol. 159: 316─322.

Harman, G.E., C.K. Hayes, M. Lorito, R.M. Broadway, A. Di Pietro, C. Peterbauer & A. Tronsmo. 1993. Chitinolytic Enzymes of Trichoderma harzianum: Purification of Chitobiosidase and Endochitinase. Phytopathology 83: 313─318.

Hartree, E.F. 1972. Determination of Protein: A Modification of the Lowry Method that Gives a Linier Photometric Response. Anal. Biochem. 48: 422─427.

Lorito, M. 1998. Chitinolitic Enzymes and Their Genes, p. 73─99 In Harman, G.E., and C.P. Kubicek. Trichoderma and Gliocladium Vol. 2. Enzymes, Biological Control and Commercial Application. Taylor and Francis, London.

Manczinger, L. & G. Polner. 1985. Cluster Analysis of Carbon Source Utilization Patterns of Trichodema Isolates. Sys. Appl. Microbiol. 9: 2 14─217.

Tronsmo, A. & G.E. Harman. 1993. Detection and Quantification of N-acetyl -D-glucosamidinase, Chitobiosidase and Endochitinase in Solutions and on Gels. Anal. Biochem. 208: 74─79.

Vessey, J.C. & G.F. Pegg. 1973. Autolysis and Chitinase Production in Cultures of Verticillium alboatrum. Trans. Br Mvcol. Soc. 60: 133─143.

Widyastuti, S.M., Sumardi, A. Sulthoni & Harjono. I 998a. Pengendalian Hayati Penyakit Akar Merah pada Akasia dengan Trichoderma. Jurnal Perlindungan Tananian Indonesia 4 (2): 65─72.

Widyastuti, S.M., Sumardi & N. Hidayati. 1998b. Kemampuan Trichoderma spp. untuk Pengendalian Hayati Jamur Akar Putih pada Acacia inangiuin secara in vitro. Bul. Kehut. 36: 24─38.

Widyastuti, S.M., Sumardi & Harjono. 1999. Potensi Antagonistik Tiga Trichoderma spp. terhadap Delapan Penyakit Akar Tanaman Kehutanan. Bul. Kehut. 41: 2─10.



DOI: https://doi.org/10.22146/jpti.10021

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