Pemanfaatan tepung daun kelor (Moringa oleifera Lamk) dengan pretreatment asam dan tepung ikan lele terhadap pemulihan anemia secara in vivo

Ayutha Wijiindyah; Syaiful Anwar; Sri Hetty Susetyorini

doi:10.22146/ijcn.15382

Pemanfaatan tepung daun kelor (Moringa oleifera Lamk) dengan pretreatment asam dan tepung ikan lele terhadap pemulihan anemia secara in vivo

https://doi.org/10.22146/ijcn.15382

Ayutha Wijiindyah^(1*), Syaiful Anwar⁽²⁾, Sri Hetty Susetyorini⁽³⁾

(1) Program Studi Ilmu Gizi, Fakultas Kedokteran Universitas Diponegoro
(2) Program Studi Ilmu Gizi, Fakultas Kedokteran Universitas Diponegoro
(3) Program Studi Ilmu Gizi, Fakultas Kedokteran Universitas Diponegoro
(*) Corresponding Author

Abstract

Background: It is estimated more than 50% of the cases of anemia in the world is caused by iron (Fe) deficiency, it needs alternative sources local food that rarely used but cheap.

Objective: This monofactorial experiment was done to find out whether kelor leaves powder resulted from acid pretreatment and addition of catfish powder can support the recovery of iron deficiency anemia, based on an in vivo study with iron (Fe) and hemoglobin (Hb) measurements.

Methods: The experiment was a trial of anemia recovery by providing 42 wistar rats with no iron-containing feeding for 7 days, then continued for 14 days with either one of six kinds of feeding: standard feeding of AIN-93 M, kelor leaves powder or catfish powder (as control), kelor leaves powder with 0.5% citric acid pretreatment, kelor leaves powder with 0.5% citric acid pretreatment and catfish powder (1:1), kelor leaves powder with 0.5% lime pretreatment, or kelor leaves powder with 0.5% lime pretreatment and catfish powder (1:1). Criteria of anemia recovery was Fe value > 80 µg/dl and Hb > 10 g/dl. Statistical analysis was performed by using ANCOVA and LSD test.

Results: The in vivo study showed that feeding rats with kelor leaves powder (respective Fe and Hb value was 101.22 µg/dl and 11.17 µg/dl), with 0.5% citric acid pretreatment (respective Fe and Hb value was 90.36 µg/dl and 10.68 µg/dl), with 0.5% lime pretreatment (respective Fe and Hb value was 115.51 µg/dl and 12.12 µg/dl), with 0.5% lime pretreatment and catfish powder (1:1) (respective Fe and Hb value was 107.29 µg/dl and 11.25 µg/dl), can be used in anemia recovery.

Conclusion: The best result for anemia recovery was obtained from feeding of kelor powder leaves with 0.5% lime pretreatment.

Keywords

iron deficiency anemia; iron; haemoglobin; acid pretreatment; Moringa oleifera Lamk

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References

Almatsier. Gizi seimbang dalam daur kehidupan. Jakarta: Gramedia Pustaka Utama; 2011.

Arisman. Buku ajar ilmi gizi: gizi dalam daur kehidupan (edisi 2). Jakarta: Penerbit EGC; 2010.

Gibney M, Susan LN, Aedin C, Hester HV. Introduction to human nutrition. second edition 2nd. USA: Wiley – Blackwell; 2009.

Gibson RS. Principles of nutritional assessment. USA: Oxford University; 2005.

Katharinia N, Jonni MS, Sitorus M. Cegah malnutrisi dengan kelor. Yogyakarta: Kanisius; 2008.

Doerr B, Lindsay C. Moringa leaf powder. USA: ECHO Technical Note; 2005.

Hotz C, Gibson RS. Traditional food – processing and preparation practices to enhance the bioavailability of micronutrients in plant – based diets. J Nutr 2007;137(4):1097-100.

Wardlaw, Hampl. Perspectives in nutrition. USA: McGraw Hill International Edition; 2007.

Williams P, Cloete T. The production and use of citric acid for the removal of potassium from the iron ore concentrate of the Sishen Iron Ore Mine, South Africa. S Afr J Sci 2010;106:1-5.

Etchevery P, Hawthorne KM, Liang LK, Abrams SA, Griffin IJ. Effect of beef and soy proteins on the absorption of non-heme iron and inorganic zinc in children. J Am Coll Nutr 2006;25(1):34-40.

Miles RD, Jacqueline PJ. Fishmeal in poultry diets: understanding the production of this valuable feed ingredient. USA: Universitas of Florida; 2011.

Rodriguez SC, Hotz C, Rivera JA. Bioavailable dietary iron is associated with hemoglobin concentration in Mexican preschool children. J Nutr 2007;137(10):2304-10.

Nakano T, Goto T, Nakaji T, Aoki T. Bioavailability of iron-fortified whey protein concentrate in iron-deficient rats. Asian-Aust J Anim Sci 2007;20(7):1120-6.

Rucker R, Storms D. Interspices comparisons of micronutrient requirements: metabolic vs absolute body size. J Nutr 2002;132(10):2999-3000.

Tatala S, Ndossi G, Ash D, Mamiro P. Effect of germination on finger millet on nutritional value on foods and effect of food suppelement on nutrition and anemia status in Tanzanian children. Tanzan Health Res Bull 2007;9(2):77-86.

Hess CT. Monitoring laboratory values: glucose, hemoglobin, hematocrit, and iron. USA: Lippincott Williams & Wilkins; 2009.

Mahima, Verma AK, Kumar A, Rahal A, Kumar, Roy D. Inorganic versus organic selenium supplementation: a review. Pakistan J Bio Sci 2012;15(9):418-25.

Bastos DM, Monaro E, Siguemoto E, Sefora M. Maillard reaction products in processed food : pros and cons. [serial online] 2012 [cited 2012 Feb 22]. Available from: URL: http://www.intechopen.com/books/food-industrial-processes-methods-and-equipment

Debnath S. Clarias batrachus, the medical fish: an excellent candidate for aquaculture & employment generation. Singapore: International Conference in Asia Agriculture and Animal; 2011.

Songklanakarin. Effect of acid and alkaline solubilition on the properties of surimi based film. J Sci Technol 2005;27(3):563-74.

Womeni HM, Tiencheu B, Linder M, Nabayo EMC, Tenyang N, Mbiapo FT, Villeneuve P, Fanni J, Parmentier M. Nutritional value and effect of cooking, drying, and storage process on some functional properties of rhychophorus phoenicis. Intr J Life Sci & Pharma Resch 2012;2(3):203-19.

DOI: https://doi.org/10.22146/ijcn.15382