Efek asupan vitamin D terhadap tekanan darah sistolik tikus putih (Rattus norvegicus)


Dessy Hermawan(1*), Sri Kadarsih(2), Sunarti .(3), Indwiani Astuti(4), Zaenal Arifin Nang Agus(5)

(1) Fakultas Kesehatan Masyarakat Universitas Malahayati
(2) Bagian Fisiologi, Fakultas Kedokteran Universitas Gadjah Mada
(3) Bagian Biokimia, Fakultas Kedokteran Universitas Gadjah Mada
(4) Bagian Farmakologi, Fakultas Kedokteran Universitas Gadjah Mada
(5) Bagian Biokimia, Fakultas Kedokteran Universitas Gadjah Mada
(*) Corresponding Author


Background: Hypertension still became potential life threatening disease for human life. The prevalence of hypertension was high and tends to increase. Previous study suggested that there was a linier rise in blood pressure at increasing distances from the equator. More recent study indicated that vitamin D insuffi ciency may be associated with elevated blood pressure.

Objective: To investigate the effect of vitamin D intake on blood pressure.

Method: This was an experimental study with pre-post test group design. The subjects were 20 male Rattus norvegicus aged 8 weeks. Subjects were divided into 5 groups and each groups received treatment as follow: lived in dark cage and consumed vitamin D free diet for 1 – 13 days, then moved to normal cage (dark-light period) and given vitamin D per-oral of 0.25 μg/kg BW for 1 – 5 days. Data of systolic blood pressure was collected and analyzed with paired t test.

Results: The absence of vitamin D in diet caused a signifi cant increase in systolic blood pressure (p=0.01) after 7 days of treatment in dark cage. While oral vitamin D supplementation of 0.25 μg/kg BW caused a signifi cant decline of systolic blood pressure since the fi rst day of vitamin D supplementation and the result appeared stronger after 4 days of treatment (p=0.001).

Conclusion: Vitamin D intake had a strong infl uence on systolic blood pressure


hypertension; vitamin; systolic blood pressure

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DOI: https://doi.org/10.22146/ijcn.15363

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