Efek asupan vitamin D terhadap tekanan darah sistolik tikus putih (Rattus norvegicus)


Dessy Hermawan(1*), Sri Kadarsih(2), Sunarti .(3), Indwiani Astuti(4), Zaenal Arifin Nang Agus(5)

(1) Fakultas Kesehatan Masyarakat Universitas Malahayati
(2) Bagian Fisiologi, Fakultas Kedokteran Universitas Gadjah Mada
(3) Bagian Biokimia, Fakultas Kedokteran Universitas Gadjah Mada
(4) Bagian Farmakologi, Fakultas Kedokteran Universitas Gadjah Mada
(5) Bagian Biokimia, Fakultas Kedokteran Universitas Gadjah Mada
(*) Corresponding Author


Background: Hypertension still became potential life threatening disease for human life. The prevalence of hypertension was high and tends to increase. Previous study suggested that there was a linier rise in blood pressure at increasing distances from the equator. More recent study indicated that vitamin D insuffi ciency may be associated with elevated blood pressure.

Objective: To investigate the effect of vitamin D intake on blood pressure.

Method: This was an experimental study with pre-post test group design. The subjects were 20 male Rattus norvegicus aged 8 weeks. Subjects were divided into 5 groups and each groups received treatment as follow: lived in dark cage and consumed vitamin D free diet for 1 – 13 days, then moved to normal cage (dark-light period) and given vitamin D per-oral of 0.25 μg/kg BW for 1 – 5 days. Data of systolic blood pressure was collected and analyzed with paired t test.

Results: The absence of vitamin D in diet caused a signifi cant increase in systolic blood pressure (p=0.01) after 7 days of treatment in dark cage. While oral vitamin D supplementation of 0.25 μg/kg BW caused a signifi cant decline of systolic blood pressure since the fi rst day of vitamin D supplementation and the result appeared stronger after 4 days of treatment (p=0.001).

Conclusion: Vitamin D intake had a strong infl uence on systolic blood pressure


hypertension; vitamin; systolic blood pressure

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Ong KL, Cheung BM, Man YB, Lau CP, Lam KS. Prevalence awareness, treatment, and control of hypertension among US adults 1999-2004. Hypertension 2007;49:69-75.

Rahajeng E , Tuminah S. Prevalensi hipertensi dan deternimannya di Indonesia. Majalah Kedokteran Indonesia 2009;59(12):580-7.

Smeltzer SC, Bare BG. Textbook of medical-surgical nursing. Jakarta: Penerbit Buku Kedokteran EGC; 2002.

Ma J, Stafford RS. Screening, treatment and control of hypertension in US private physician officer 2003-2004. Hypertension 2008;51:1275-81.

Rostand SG. Ultraviolet light may contribute to geographic and racial blood pressure differences. Hypertension 1997;30(2 Pt 1):150-6.

Li YC. Vitamin D regulation of the renin angiotensin system: mechanism and implication. [series online] 2007 [cited 2011 July 20]. Available from: http://www.unimet.edu/cin2007/.

Forman JP, Giovannucci E, Holmes MD, Ferrari HAB, Tworoger SS, Willett WC, Curhan GC. Plasma 25-hydroxyvitamin D levels and risk of incident hypertension. Hypertension 2007;49(5):1063-9.

Martini LA, Wood RJ. Vitamin D and blood pressure connection: update on epidemiologic, clinical, and mechanistic evidence. Nutr Rev 2008;66(5):291-7.

Heaney RP. Vitamin D in health and disease. Clin J Am Soc Nephrol 2008;3(5):1535-41.

Li YC, Kong J, Wei M, Chen ZF, Liu SQ, Cao LP. 1,25-Dihydroxyvitamin D(3) is a negative endocrine regulator of the renin-angitensin system. J Clin Invest 2002;110(2):229-38.

Outila TA, Karkkainen MUM, Lamberg-Allardt CJE. Vitamin D status affect serum parathyroid hormone concentration during winter in female adolescents: associations with forearm bone mineral density. Am J Clin Nutr 2001:74(2):206-10.

Judd SE, Nanes MS, Ziegler TR, Wilson PWF, Tangpricha V. Optimal vitamin D status the age-associated increase in systolic blood pressure in white Americans: result the third national health and nutrition examination survey. Am J Clin Nutr 2008;87:136-41.

Yuan W, Pan W, Kong J, Zheng W, Szeto FL, Wong KE, Cohen R, Klopot A, Zhang Z, Li YC. 1,25-dihydroxyvitamin D3 suppresses renin gene transcription by blocking the activity of the cyclic AMP response element in the renin gene promoter. J Biol Chem 2007;282(41):29281-30.

Barret KE, Brooks H, Baitano S, Barman SM. Ganong’s review of medical physiology 23 edition. USA: Mc-Graw Hill-Lange; 2010.

Hall JE. Guyton and hall review physiology. Philadelphia: Sauders-Elsevier; 2006.

Sherwood L. Human physiology from cell to systems 7 edition. Canada: Yolanda Cassio; 2010.

Kimura Y, Kawamura M, Owada M. Effectiveness of 1,25-dehydroxyvitamin D supplementation on blood pressure reduction in a pseudohypoparathyroidism patient with high renin activity. Intern Med 1999;38:31-5.

Sun Q, Shi L, Rimm EB, Giovannucci EL, Hu FB, Manson JE, Rexrode KM. Vitamin D intake and risk of cardiovascular disease in US men and women. Am J Clin Nutr 2011;94:534-42.

Harris RC, Breyer MD. Physiological regulation of cyclooxygenase-2 in the kidney. Am J Physiol Renal Physiol 2001;281(1):F1-11.

Bell PD, Komlosi P, Zhang ZR. ATP as a mediator of macula densa cell signalling. Purinergic Signal 2009;5(4):461-71.

DOI: https://doi.org/10.22146/ijcn.15363

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