* Corresponding Author
Antioxidant Activity and Phytochemical Screening of Rhizopora mucronata Mangrove Leaf Extract from Mangrove Botanical Garden, Surabaya City, Esat Java
https://doi.org/10.22146/jfs.90770 Rahmawati Rahmawati(1*), Yushinta Aristina Sanjaya(2), Yunita Satya Pratiwi(3), Elistiya Putri Nuraini Hendrawan(4), Zhafa Nesya Salsabila(5), Tania Amalia(6)
(1) Department of Food Technology, Faculty of Engineering, University of Pembangunan Nasional Veteran Jawa Timur, Surabaya, East Java
(2) Department of Food Technology, Faculty of Engineering, University of Pembangunan Nasional Veteran Jawa Timur, Surabaya, East Java
(3) Department of Food Technology, Faculty of Engineering, University of Pembangunan Nasional Veteran Jawa Timur, Surabaya, East Java
(4) Department of Food Technology, Faculty of Engineering, University of Pembangunan Nasional Veteran Jawa Timur, Surabaya, East Java
(5) Department of Food Technology, Faculty of Engineering, University of Pembangunan Nasional Veteran Jawa Timur, Surabaya, East Java
(6) Department of Food Technology, Faculty of Engineering, University of Pembangunan Nasional Veteran Jawa Timur, Surabaya, East Java
(*) Corresponding Author
Abstract
Rhizophora mucronata is a type of mangrove plant that acts as a natural antioxidant which is used as a food ingredient and traditional medicine. The aim of this research was to determine the profile of secondary metabolites, antioxidant activity and total phenols of R. mucronata leaf extract. R. mucronata leaves were obtained from Gunung Anyar District, Surabaya City and then extracted using three solvents with different polarities, namely n-hexane, ethyl acetate, and ethanol. Mangrove leaf extract (Rhizopora mucronata) was followed by phytochemical testing using color testing, antioxidant activity testing using the DPPH method, and determining phenol content using the Folin-Ciocalteu method. Phytochemical tests show that all R. mucronata mangrove leaf extracts contain secondary metabolites such as flavonoids, saponins, steroids, triterpenoids and tannins with total phenols ranging from 0.04%-6.7%. The antioxidant activity of n-hexane, ethyl acetate, and ethanol extracts were 4.9 mg ± 0.82%, 1.7 mg ± 0.17%, and 0.21 mg ± 1.9%, respectively.
Keywords
References
Abdi, V., I. Sourinejad, M. Yousefzadi & Z. Ghasemi. 2019. Biosynthesis of silver nanoparticles from the mangrove Rhizophora mucronata: Its characterization and antibacterial potential. Iranian Journal of Science and Technology, Transaction A: Science. 43 (5): 2163-2171. https://doi.org/10.1007/s40995-019-00739-9
Aji, A.P., E. Issusilaningtyas, A.R. Fauziah & D.R. Nugraheni. 2023. Pengaruh metode ekstraksi terhadap kandungan metabolit sekunder ekstrak daun bakau hitam (Rhizopora mucronata). Sains Indonesiana. 1 (3): 173-181.
Akasia, A.I., I.D.N.N. Putra & I.N.G. Putra. 2021. Skrining fitokimia ekstrak daun mangrove Rhizophora mucronata dan Rhizophora apiculata yang dikoleksi dari kawasan mangrove Desa Tuban, Bali. JMRT. 4 (1): 16-22. https://ojs.unud.ac.id/index.php/JMRT
Angio, M.H., E. Renjana, L.W. Ningrum, E.R. Firdiana & R. Irawanto. 2022. Inventory of plants in the Mangrove Botanic Garden of Gunung Anyar and their potential as medicinal plants. Jurnal Penelitian Kehutanan Wallacea. 11 (1): 53-70. www.jurnal.balithutmakassar.org
Bulan, D.E., N. Nurfadilah, M.R. Syahrir, A. Mismawati, A.K. Torambung & M. Rachmawati. 2022. Phytochemical composition and antioxidant activity of leaf extracts from three Rhizophora species from Bontang Waters, Indonesia. Tropical Journal of Natural Product Research. 6 (8): 1178-1182. https://doi.org/10.26538/tjnpr/v6i8.2
Dia, S.P.S., N. Nurjanah & A.M. Jacoeb. 2015. Chemical composition, bioactive components and antioxidant activities from root, bark and leaf lindur. Jurnal Pengolahan Hasil Perikanan Indonesia. 18 (2): 205-219. https://doi.org/10.17844/jphpi.2015.18.2.205
Egra, S., H. Kuspradini, I.W. Kusuma, I. Batubara, I. Imra, N. Nurjannah, E. Wahyuni, K. Yamauchi & T. Mitsunaga. 2023. Potential of prospective medicinal plants of Rhizophoraceae from North Kalimantan, Indonesia. Biodiversitas. 24 (3): 1346-1355. https://doi.org/10.13057/biodiv/d240303
Filbert, F., H.S.J. Koleangan, M.R.J. Runtuwene & V.S. Kamu. 2014. Penentuan aktivitas antioksidan berdasarkan nilai LC50 ekstrak metanol dan fraksi hasil partisinya pada kulit biji pinang yaki (Areca vestiaria Giseke). Jurnal MIPA UNSRAT. 3 (2): 149-154.
Hardiningtyas, S.D., S. Purwaningsih & E. Handharyani. 2014. Antioxidant activity and hepatoprotective effect of green mangrove leaves. JPHPI. 17 (1): 80-91.
Haryoto, H & A. Frista. 2019. Aktivitas antioksidan ekstrak etanol, fraksi polar, semipolar dan non polar dari daun mangrove kacangan (Rhizophora apiculata) dengan metode DPPH dan FRAP. J. Sains Kes. 2 (2): 131-138. https://doi.org/10.25026/jsk.v2i2.129
Hastuti, E.D., M. Izzati & S. Darmanti. 2023. Usability of planted mangroves in the Coastal Area of Semarang, Indonesia, as the source of secondary metabolite extracts. Biodiversitas. 24 (4): 2409-2415. https://doi.org/10.13057/biodiv/d240456
Indriaty, I., D. Djufri, B. Ginting & K. Hasballah. 2023. Phytochemical screening, phenolic and flavonoid content, and antioxidant activity of rhizophoraceae methanol extract from Langsa, Aceh, Indonesia. Biodiversitas. 24 (5): 2865-2876. https://doi.org/10.13057/biodiv/d240541
Lasabuda, R. 2013. Pembangunan wilayah pesisiran dan lautan dalam perspektif Negara Kepulauan Republik Indonesia. Jurnal Ilmiah Platax. 1 (2): 92-101.
Leksono, W. B., R. Pramesti, G.W. Santosa & W.A. Setyati. 2018. Jenis pelarut metanol dan N-Heksana terhadap aktivitas antioksidan ekstrak rumput laut Gelidium sp. dari Pantai Drini Gunung kidul - Yogyakarta. Jurnal Kelautan Tropis. 21 (1): 9-16. https://doi.org/10.14710/jkt.v21i1.2236
Lesdiana, L & U. Usman. 2021. Uji toksisitas dan uji fitokimia ekstrak metanol daun mangrove Rhizophora mucronata. Prosiding Seminar Nasional Kimia. 94-98.
Li, H.F., L.J. Li., L.M. Ji, X.W. Peng & W. Li. 2019. The extraction ability and mechanism in extraction lithium by several organic extractants. Chemical Physics Letters. 733. https://doi.org/10.1016/j.cplett.2019.136668
Liu, Z., Z. Ren, J. Zhang, C.C. Chuang, E. Kandaswamy, T. Zhou & L. Zuo. 2018. Role of ROS and Nutritional Antioxidants in Human Diseases. Frontiers in Physiology. 9 (477): 1-14.
Loho, R.E.M., M. Tiho & Y.A. Assa. 2021. Kandungan dan aktivitas antioksidan pada rumput laut merah. Medical Scope Journal. 3( 1): 113-120. https://doi.org/10.35790/msj.3.1.2021.34986
Manteu, S.H., N. Nurjanah & T. Nurhayati. 2018. Characteristics of brown seaweeds Sargassum policystum and Padina minor from Pohuwato Water, Gorontalo. Jurnal Pengolahan Hasil Perikanan Indonesia. 21 (3): 296. https://doi.org/10.17844/jphpi.v21i3.24709
Manuhuttu, D & N.A. Saimima. 2021. Potensi daun mangrove (Sonneratia alba) sebagai antibakteri terhadap Salmonella, Staphylococcus aureus, dan Escherichia coli. Biopendix. 7 (2): 71-79.
Maulana, D.M & B.B. Sasmito. 2021. The dose effect of mangrove leaf extract (Rhizophora apiculata) on anticancer activity in HeLa cells. Journal of SCRTE. 5 (1): 1-15.
Mile, L., H. Nusyam, D. Setijawati & P.D. Sulistiyati. 2021. Flavonoid content of mangrove Rhizhopora mucronata in Langge Village, District Anggrek, North Gorontalo. GOSPODARKA I INNOWACJE. 17: 1-4.
Moito, R.A.A., R. Husain & A.S. Naiu. 2023. Analisis kadar saponin dan aktivitas antioksidan ekstrak daun mangrove Sonneratia alba dari Perairan Desa Monano Kabupaten Gorontalo Utara. Jurnal Ilmiah Perikanan dan Kelautan. 11 (2): 92-96.
Nasution, A.D.M., U. Amna & H. Halimatussakdiah. 2019. Skrining fitokimia daun jarak pagar (Jatropha curcas L.) dari Kota Langsa. Jurnal Kimia Sains dan Terapan. 1 (1): 11-15.
Permana, A.H.C., A. Husni & S.A. Budhiyanti. 2016. Antioxidant activity and toxicity of seagrass Cymodocea sp. extracts. Jurnal Teknologi Pertanian. 17 (1): 37-46.
Plaza, C.M., L.E.D. De. Torres, R.K., Lücking, M. Vizcaya & G.E. Medina. 2014. Antioxidant activity, total phenols and flavonoids of lichens from Venezuelan Andes. Journal of Pharmacy & Pharmacognosy Research. 2 (5): 138-147. http://jppres.com/jppres
Prasedya, E.S., N.W.R. Martyasari, R. Apriani, S. Mayshara,R.A. Fanani & H. Sunarpi. 2019. Antioxidant activity of Ulva lactuca L. from different Coastal Locations of Lombok Island, Indonesia. AIP Conference Proceedings. 2199: 1-6. https://doi.org/10.1063/1.5141281
Prayitno, S.A., J. Kusnadi & E.S. Murtini. 2016. Antioxidant activity of red betel leaves extract (Piper crocatum Ruiz & Pav.) By difference concentration of solvents. Research Journal of Pharmaceutical Biological and Chemical Sciences. 7 (5): 1836-1843. https://www.researchgate.net/publication/320755645
Purwaningsih, S., E. Salamah, A.Y.P. Sukarno & E. Deskawati. 2013. Aktivitas antioksidan dari buah mangrove (Rhizophora mucronata Lamk.) pada suhu yang berbeda. JPHPI, 16 (3): 199-206.
Rahadian, A., B. Prasetyo, Y. Setiawan & K. Wikantika. 2019. A historical review of data and information of indonesian mangroves area. Media Konservasi. 24 (2): 163-178.
Ridlo, A., R. Pramesti, E. Supriyantini & N. Soenardjo. 2017. Aktivitas antioksidan ekstrak daun mangrove Rhizopora mucronata. Buletin Oseanografi Marina. 6 (2): 110-116. http://ejournal.undip.ac.id/index.php/buloma
Rumengan, A.P., E.S. Mandiangan, W.A. Tanod, D.S.J. Paransa, C.P. Paruntu & D.M.H. Mantiri. 2021. Identification of pigment profiles and antioxidant activity of Rhizophora mucronata mangrove leaves Origin Lembeh, North Sulawesi, Indonesia. Biodiversitas, 22 (7): 2805-2816. https://doi.org/10.13057/biodiv/d220730
Sachithanandam, V., P. Lalitha, A. Parthiban, T. Mageswaran, K. Manmadhan & R. Sridhar. 2019. A review on antidiabetic properties of indian mangrove plants with reference to island ecosystem. Evidence-Based Complementary and Alternative Medicine. 2019. https://doi.org/10.1155/2019/4305148
Sadeer, N.B., G. Rocchetti, B. Senizza, D. Montesano, G. Zengin, A. Uysal, R. Jeewon, L. Lucini & M.F. Mahomoodally. 2019. Untargeted metabolomic profiling, multivariate analysis and biological evaluation of the true mangrove (Rhizophora mucronata lam.). Antioxidants. 8 (10): 1-20. https://doi.org/10.3390/antiox8100489
Sari, P., D.E. Embong & E. Marliana. 2019. Phytochemical screening and identification mangrove on Panrita Lopi Beach Muara Badak. Jurnal Aquarine. 6 (1): 58-66.
Saxena, M., J. Saxena, R. Nema, D. Singh & A. Gupta. 2013. Phytochemistry of medicinal plants. Journal of Pharmacognosy and Phytochemistry. 1(6): 168-182. www.phytojournal.comwww.phytojournal.com
Setyawan, A.D., P. Ragavan, M. Basyuni & S. Sarno. 2022. Review: Rhizophora mucronata as source of foods and medicines. International Journal of Bonorowo Wetlands. 9(1): 42-55. https://doi.org/10.13057/bonorowo/w090105
Sopalun, K., W. Laosripaiboon, A. Wachirachaikarn & S. Iamtham. 2021. Biological potential and chemical composition of bioactive compounds from endophytic fungi associated with thai mangrove plants. South African Journal of Botany. 141: 66-76. https://doi.org/10.1016/j.sajb.2021.04.031
Suhendra, C.P., I.W.R. Widarta & A.A.I.S. Wiadnyani. 2019. The effect of ethanol concentration on antioxidant activity of cogon grass rhizome (Imperata cylindrica (Linn.) Beuv.) extract using ultrasonic wave. Jurnal Ilmu Dan Teknologi Pangan. 8 (1): 27-35.
Syamsudin, S., A.H. Alimuddin & B. Sitorus. 2022. Isolation and characterization of phenolic compound from putat leaves (Planchonia valida Blume). Indonesian Journal of Pure and Applied Chemistry. 5 (2): 85-98. https://doi.org/10.26418/indonesian.v5i2.56554
Triyatno, T., F. Febriandi, A. Putra & E. Kamal. 2019. Identification of physical characteristics and the change of mangrove region in Coastal Southern Part of Padang City West Sumatra-Indonesia. Geography and Geography Education. 3 (1): 87-93. http://sjdgge.ppj.unp.ac.id
Usman, U., D. Fildzania & I. Fauzi. 2022. Uji aktivitas antioksidan dan antidiabetes ekstrak daun mangrove Rhizopora mucronata. Jurnal Sains Dan Kesehatan. 4 (1): 28-35. https://doi.org/10.25026/jsk.v4i1.724
Yang, L., K.S. Wen, X. Ruan, Y.X. Zhao, F. Wei & Q. Wang. 2018. Response of plant secondary metabolites to environmental factors. Molecules. 23 (4): 1-26. https://doi.org/10.3390/molecules23040762
DOI: https://doi.org/10.22146/jfs.90770
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