ISOLASI, IDENTIFIKASI DAN KARAKTERISASI Vibrio spp. PATOGEN PENYEBAB VIBRIOSIS PADA KERAPU DI BALAI BUDIDAYA AIR PAYAU SITUBONDO

https://doi.org/10.22146/jfs.9053

Kamiso Handoyo Nitimulyo(1*), Alim Isnansetyo(2), Triyanto Triyanto(3), Indah Istiqomah(4), Muhammmad Murdjani(5)

(1) Jurusan Perikanan Fakultas Pertanian UGM
(2) Jurusan Perikanan Fakultas Pertanian UGM
(3) Jurusan Perikanan Fakultas Pertanian UGM
(4) Jurusan Perikanan Fakultas Pertanian UGM
(5) Balai Besar Pengembangan Budidaya Air Payau, Jepara
(*) Corresponding Author

Abstract


This research was conducted to isolate, identify, and characterize pathogenic Vibrio spp., causative agents of vibriosis in grouper at Brackishwater Aquaculture Development Center (BADC), Situbondo. Twenty-nine isolates were isolated from wound, gills, eyes, liver, and ren of grouper with vibriosis on Thiosulphate Citrate Bile Sucrose Agar (TCBSA) medium. Koch Postulate Test was conducted to determine pathogenic Vibrio spp., by intraperitoneal injection to humpback grouper (Cromileptes altivelis) (9-10 cm of total length) at 106 cells/fish in triplicates. Results indicated that the pathogenic Vibrio spp., causative agents of vibriosis in grouper at BADC, Situbondo were identified to be V. alginolyticus, V. anguillarum, V. metchnikovii, V. vulnificus, V. fluvialis, V. furnisii, and V. parahaemolyticus. Infection of the pathogenic Vibrio spp. caused acute mortality within 17-46 hours with specific disease signs like haemorhagic on fins (pinnae pectorales, pinnae abdominales, pinna analis) and also on the body. These results suggested that vibriosis should be monitored and controlled properly and quickly.

Keywords


humpback grouper (Cromileptes altivelis), Koch postulate, pathogenic Vibrio, vibriosis

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References

Austin, B. and D.A. Austin. 1987. Bacterial fish pathogens: disease in farmed and wild fish. John Wiley and Sons. Chichester. 364 p.

Esteve, C., C. Amaro, E.G. Biosca, and E. Garay. 1995. Biochemical and toxigenic properties of Vibrio furnisii isolated from an european eel farm. Aquaculture. 132: 81-90.

Gerhardt, P., R.G.E. Murray, W.A. Wood, and N.R. Krieg. 1994. Methods for general and molecular bacteriology. American Society for Microbiology. Washington. 651 p.

Hastein, T. and J.E. Smith. 1977. A Study of Vibrio anguillarum from farmed and wild fish using principal components analysis. J. Fish. Biol. 11: 69-75.

Holt, J.G., P.H.A. Sneath, J.T. Stanley, and S.T. Williams. 1994. Bergey’s manual of determinative bacteriology. 9th Ed. Williams and Wilkins. Baltimore. 787 p.

Inglis, V., R.J. Robert, and N.R. Bromage. 1993. Bacterial disease of fishes. Blackwell Scientific Pub. London. 283 p.

Jordan, E.O. and W. Borrows. 1941. Text book of bacteriology. 40 Ed. Saunders Company. Philadelphia. 456 p.

Jutono, J. Soedarsono, S. Hartadi, S. Kabirun, S. Suhadi, dan D. Soesanto. 1973. Pedoman praktikum mikrobiologi umum untuk perguruan tinggi. Departemen Mikrobiologi Fakultas Pertanian. Universitas Gadjah Mada. 153 p.

Lightner, D.V. 1996. A handbook of shrimp pathology and diagnostic procedures for diseases of culture penaeid shrimp. Sec. 4: Bacteria, vibriosis-culture and identification. The World Aquaculture Society. Baton Rouge, Lousiana. 26 p.

MacFaddin, J.F. 1980. Biochemical test for identification of medical bacteria. Second Ed. Williams and Wilkins. Baltimore. 528 p.

Murdjani, M. 2002. Identifikasi dan patologi bakteri Vibrio alginolyticus pada ikan kerapu tikus (Cromileptes altivelis). Ringkasan Disertasi. Universitas Brawijaya. Malang. 48p.

Muroga, K., K. Satoh, and T. Wakal. 1984. Growth temperature of five bacterial pathogen for eel. Fish Pathol. 19(3): 193-196.

Rollins, D.M. and S.W. Joseph. 2000. List of bacterial pathogen. BSCL 424 Pathogenic Microbiology. University of Maryland. http://www. life.umd.Edu/classroom/bci 434/ index.html. Diakses tanggal 17 Agustus 2004.

Sarono, A. Widodo, dan E.B. Sri Haryani. 1993. Deskripsi hama dan penyakit ikan karantina golongan bakteri. Edisi kedua. Pusat karantina Perta-nian dan Fakultas Pertanian Jurusan Perikanan UGM. Yogyakarta. 90 p.

Schiewe, M.H., T.J. Trust, and J.H. Crosa. 1982. Vibrio ordalii sp. Nov. Int. J. Syst. Bacteriol. 32: 384-384.

Schaperclause, W. 1991. Fish disease 1. A.A. Balkema/Rotterdam. 588 p.

Sudheesh, P.S. and H-S Xu. 2001. Patho-genicity of Vibrio parahaemolyticus in tiger prawn Penaeus monodon Fabricius: possible role of extracellular proteases. Aquaculture. 196: 37-46.

Sung, H.H., G.H. Kou, and Y.L. Song. 1993. Characterization of mono-clonal antibodies and corresponding epitopes of Vibrio vulnificus. Fish Pathol. 28(4): 181-188.

Taslihan, A., M. Murdjani, C. Purbomartono, dan E. Kusnendar. 2000. Bakteri patogen penyebab penyakit mulut merah pada ikan kerapu tikus (Cromileptes altivelis). J. Perikanan. II(2): 57-62.

Wijayanti, A. dan N. Hamid. 1997. Identifikasi bakteri pada pembenihan ikan kerapu tikus (Cromileptes altivelis). Ditjen. Perikanan. Deptan. 9 p.

Zafran, D. Roza, I. Koesharyani, and F. Johnny. 1998. Panduan untuk diagnosis penyakit ikan dan krustase laut di Indonesia. JICA dan Loka Penelitian Perikanan Pantai Gondol. 44 p.



DOI: https://doi.org/10.22146/jfs.9053

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Jurnal Perikanan Universitas Gadjah Mada (print ISSN 0853-6384; online ISSN 2502-5066) is published by Department of Fisheries, Universitas Gadjah Mada in collaboration with Semnaskan UGM (Seminar Nasional Tahunan Hasil Perikanan dan Kelautan) and ISMFR (International Symposium on Marine and Fisheries Research).

 

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