KOMBINASI EFEKTIF EKSTENDER DAN KRIOPROTEKTAN PADA KRIOPRESERVASI SPERMA IKAN NILEM (Osteochilus hasseltii Valenciennes, 1842)

https://doi.org/10.22146/jfs.8931

A. Sunarma(1*), D. W. B. Hastuti(2), D. M. Saleh(3), Y. Sistina(4)

(1) Program Studi Biologi, Program Pascasarjana, Universitas Jenderal Soedirman; BBPBAT Sukabumi
(2) Program Studi Biologi, Program Pascasarjana, Universitas Jenderal Soedirman
(3) Fakultas Peternakan, Universitas Jenderal Soedirman
(4) Program Studi Biologi, Program Pascasarjana, Universitas Jenderal Soedirman
(*) Corresponding Author

Abstract


Cryopreservation technology of fish sperm has been developed to prolong gamete viability. Combination of two extenders (ringer or glucose) and two cryoprotectans (DMSO or methanol) on three concentrations have studied to sperm cryopreservation of nilem. Nilem fish brooder induced by GnRHa and domperidone combination. Sperm was diluted in extender at the ratio of 1:9 then cryoprotectant was added at 5%, 10% or 15% (v/v) concentrations. Samples were stored in 0,5 mL straws, equilibrated at temperature 4 – 5 oC for 20 minutes, vaporized at 3 cm above surface liquid nitrogen for 3 minutes and then plunged into liquid nitrogen, where they were stored for 1 weeks. Sperm was thawed at temperature 39 – 40 oC for 10 – 15 sec. and was used to fertilize 100 – 200 eggs per straw. The highest percentage of post-thawed sperm was combination ringer and DMSO 10% (87.50 ± 5.00%) and the lowest was combination ringer and methanol 5% (23.75 ± 4.79%). The highest hatching rate fertilized by post-thawed sperm was combination ringer and DMSO 15% (54.98 ± 28.61%) and the lowest was combination glucose and DMSO 15% (6.54 ± 3.32%). The study proven combination of ringer extender and DMSO cryoprotectant effective for cryopreservation of Indonesian silver sharkminnow fish sperm. Therefore, fish sperm cryopreservation with nilem as a representative model could be developed to extend on other Cyprinidae.

Keywords


cryopreservation, sperm, sperm extender, nilem fish

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References

Alavi. S.M.H. and J. Cosson. 2006. Sperm Motility in Fishes (II). Effects of Ions and Osmolality: A Review. Cell Biol. Int., 30:1-14.

Babiak, I., J. Glogowski, E. Brzuska, J. Szumiec and J. Adamek. 1997. Cryopreservation of Sperm of Common Carp, Cyprinus carpio L. Aquac. Res., 28:567-571.

Basavaraja, N., and S.N. Hegde. 2004. Cryopreservation of the endangered mahseer (Tor khudree) spermatozoa: I. Effect of extender composition, cryoprotectants, dilution ratio, and storage period on post-thaw viability. Cryobiology, 49:149–156.

Billard, R. and T. Zhang. 2001. Techniques of Genetic Resource Banking in Fish. In P.F. Watson and W.V. Holt (eds.). Cryobanking the Genetic Resource: Wildlife Conservation for the Future. Taylor and Francis, London. p. 156-170.

Chao, N-H. and I.C. Liao. 2001. Cryopreservation of finfish and shellfish gametes and embryos. Aquaculture, 197:161-189.

Dong, Q., C. Huang and T.R. Tiersch. 2007. Control of Sperm Concentration is Necessary for Standardization of Sperm Cryopreservation in Aquatic Species: Evidence from Sperm Agglutination in Oysters. Cryobiology, 54:87-98.

He, S. and L.C. Woods III. 2004. Effects of Dimethyl Sulfoxide and Glycine on Cryopreservation Induced Damage of Plasma Membranes and Mitochondria to Striped Bass (Morone saxatilis) Sperm. Cryobiology, 48:254-262.

Horvath, A., E. Miskolczi and B. Urbanyi. 2003. Cryo preservation of Common Carp Sperm. Aquat. Living Resour. 16:457-460.

Horvath, A., E. Miskolczi, S. Mihalffy, K. Osz, K. Szabo and B. Urbanyi. 2007. Cryopreservation of common carp (Cyprinus carpio) sperm in 1.2 and 5 ml straws and occurrence of haploids among larvae produced with cryopreserved sperm. Cryobiology, 54:251–257.

Kopeika, J., E. Kopeika, T. Zhang, and D.M. Rawson. 2003. Studies on the Toxicity of Dimethyl Sulfoxide, Ethylene Glycol, Methanol and Glicerol to Loach (Misgurnus fossilis) Sperm and the Effect on Subsequent Embryo Development. CryoLetters, 24:365-374.

Lahnsteiner, F., B. Berger, A. Horvath, B. Urbanyi and T. Weismann. 2000. Cryopreservation of Spermatozoa in Cyprinid Fishes. Theriogenology, 54:1477-1498.

Linhart, O., M. Rodina and J. Cosson. 2000. Cryopreservation of Sperm in Common Carp Cyprinus carpio: Sperm Motility and Hatching Success of Embryos. Cryobiology, 41:241-250.

Lubzens, E., N. Daube, I. Pekarsky, Y. Magnus, A. Cohen, F. Yusefovich and P. Feigin. 1997. Carp (Cyprinus carpio L.) Spermatozoa Cryobanks – Strategies in Research and Application. Aquaculture, 155:13-30.

Muchlisin, Z.A., R. Hashim and A.S.C. Chong. 2004. Preliminary Study on the Cryopreservation of Tropical Bagrid Catfish (Mystus nemurus) Spermatozoa; the Effect of Extender and Cryoprotectant on the Motility after Short-Term Storage. Theriogenology, 62:25–34.

Rana, K. 1995. Preservation of Gametes. In N.L. Bromage and R.R. Roberts (eds.). Broodstock Management and Egg and Larval Quality. Blackwell Science. London. p. 53-75.

Routray, P., A.K. Choudhary, S.N. Dash, D.K. Verma, C. Dash, P. Swain, J.K. Jena, S.D. Gupta and N. Sarangi. 2006. Cryopreservation of Dead Fish Spermatozoa Several Hours after Death of Indian Major Carp, Labeo rohita and its Successful Utilization in Fish Production. Aquaculture, 261:1204-1211.

Sunarma, A., D.W.B. Hastuti, dan Y. Sistina. 2007. Penggunaan Ekstender Madu yang Dikombinasikan dengan Krioprotektan Berbeda pada Pengawetan Sperma Ikan Nilem (Indonesian Sharkminnow, Osteochilus hasseltii Valenciennes, 1842). Prosiding “Aquaculture Indonesia 2007”. Masyarakat Akuakultur Indonesia. Surabaya 05-07 Juni 2007. 9p.

Suquet, M., C. Dreanno, C. Fauvel, J. Cosson and R. Billard. 2000. Cryopreservation of Sperm in Marine Fish. Aquac. Res., 31:231-243.

Tiersch, T.R. 2006. Fish Sperm Cryopreservation for Genetic Improvement and Conservation in Southeast Asia. Fish for the People, 4(2):21-33.

Urbanyi, B., T. Szabo, E. Miskolczi, S. Mihalffy, K. Vranovics, and A. Horvath. 2006. Successful fertilization and hatching of four European cyprinid species using cryopreserved sperm. J. Appl. Ichthyol., 22:201–204.

Warnecke, D. and H. Pluta. 2003. Motility and Fertilizing Capacity of Frozen/Thawed Common Carp (Cyprinus carpio L.) Sperm using Dimethyl-Acetamide as the Main Cryoprotectant. Aquaculture, 215:167-185.



DOI: https://doi.org/10.22146/jfs.8931

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Jurnal Perikanan Universitas Gadjah Mada (print ISSN 0853-6384; online ISSN 2502-5066) is published by Department of Fisheries, Universitas Gadjah Mada in collaboration with Semnaskan UGM (Seminar Nasional Tahunan Hasil Perikanan dan Kelautan) and ISMFR (International Symposium on Marine and Fisheries Research).

 

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