PENGARUH DEASETILASI DAN ALKALINASI TERHADAP KARAKTERISTIK KARBOKSIMETIL KITOSAN

https://doi.org/10.22146/jfs.8929

Yusro Nuri Fawzya(1*), Rina Novianty(2), Singgih Wibowo(3)

(1) Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan
(2) Alumni Jurusan Farmasi FMIPA, Universitas Indonesia
(3) Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan
(*) Corresponding Author

Abstract


Carboxymethyl chitosan (CMCts) is one of the chitosan derivatives produced by the carboxymethylation process of chitosan. Compared to chitosan which is soluble in weak acid, the CMCts is soluble in water and it becomes more desirable in many applications both in pharmaceutical and food industries. Chitosan as the raw material of CMCts was made from chitin deacetylation. The reasearch aimed to study the effect of chitin deacetylation methods and concentrations of sodium hydroxide in the alkalination process on the characteristics of CMCts produced. Three deacetylation processes were applied in the production of chitosan, i.e. treatment with 60% NaOH at 70oC for 2 and 3 days, and treatment with 60% NaOH at ambient for 5 days. Whereas, the alkalination process was conducted using NaOH at the concentrations of 20, 30, 40 and 50%. The carboxymethylation of chitosan was carried out using monochloroacetic acid with the ratio of chitosan to monochloroacetic acid of 1 : 1.5 (w/w) at 50oC for an hour. The results showed that the CMCts produced had solubilitiy of 27.0 to 98,1% and viscosity of 28 to 265 cPs. Based on the solubility and viscosity, the best treatment was found on using chitosan which had been deacetylated with 60% NaOH at 70oC for 3 days followed by alkalination using NaOH of 30% before carboxymethylation. The treatment resulted CMCts with water solubility 98.1%, viscosity 265 cPs and yield 72.2%.

Keywords


chitosan, alkalination, carboxymethyl chitosan

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References

Anonim. 2005. Carboxymethyl chitosan. http://www.greatvistachemicals.com/biochemicals/carboxy -methylchitosan. html. Diakses tanggal 31 Januari 2005.

Bader, H.J. and E. Birkholz. 1997. Teaching chitin chemistry. In: Chitin handbook. R.A.A. Muzzarelli and M.G. Peter (Eds.). European Chitin Society. Atec. Grottammare. Italy: 507-519.

Chen, R-H., J-R. Chang, and J-S. Shyur. 1997. Effects on ultrasonic conditions and storage in acidic solutions on changes in molecular weight and polydispersity of treated chitosan. Carbohydrate Research. 299: 287-294.

Chen, L., T. Zhigang, and D. Yumin. 2004. Synthesis and pH sensitivity of carboxymethyl chitosans-based polyampholyte hydrogels for protein carriers matrices. Biomaterials. 25: 3725-3732.

Davies, D.H., C.M. Elson, and E.R. Hayes. 1988. N, O-carboxymethyl chitosan, a new water soluble chitin derivative. In: Chitin and chitosan: sources, chemistry, biochemistry, physical properties and application. Skjak-Braek, G.T. Anthonsen, and P. Sandford. (Eds.). Elsevier Applied Sci. Crownhouse. Essex. England. 467-472.

Direktorat Jenderal Perikanan Tangkap. 2006. Statistik perikanan tangkap Indonesia. Departemen Kelautan dan Perikanan. Direktorat Jenderal Perikanan Tangkap. Jakarta.

Dwiyitno, J. Basmal, dan Mulyasari. 2004. Pengaruh suhu esterifikasi terhadap karakteristik karboksimetil kitosan (CMCts). Jurnal Penelitian Perikanan Indonesia. Edisi Pasca Panen. 10(3): 67-73.

Hayes, E.R. 1986. N, O-Carboxymethyl chitosan and preparative method therefor. US Patents. 4,619,995. 7p

Joshita. 2002. Buku petunjuk praktikum farmasi fisika. Fakultas Matematika dan Ilmu Pengetahuan Alam. Universitas Indonesia. Jakarta. 29 p.

Kyoon, N.H., J.N. Won, and S.P. Meyers. 2003. Effect of time/temperature treatment parameters on depolymerization of chitosan. J. Applied Polysacharide Science. 87:1890-1894.

Lin, Y-H., H-F. Liang, C-K. Chung, M-C. Chen, and H-W. Sung. 2005. Physically crosslinked alginate/N,O-carboxymethyl chitosan hydrogels with calcium for oral delivery of protein drugs. Biomaterials. 26: 2105-2113.

Manguiat, L.S., C. V. Sabularse, and D.C. Sabularse. 2001. Development of carboxymethylcellulose from nata de coco. ASEAN Journal on Science and Technology for Development.18 (2): 88-94.

Muzzarelli, R.A.A., R. Rocchetti, V. Stanic, and M. Weckx. 1997. Methods for the determination of degree of acetylation of chitin and chitosan. In: Chitin handbook. R.A.A. Muzzarelli and M.G. Peter (Eds.). European Chitin Society. Atec, Grottammare, Italy.109-132.

Oktavia, D.A., S. Wibowo, dan Y.N. Fawzya. 2005. Pengaruh jumlah monokhloroasetat terhadap karakteristik karboksimetil kitosan dari kitosan cangkang dan kaki rajungan. Jurnal Penelitian Perikanan Indonesia. 11(4): 79-88.

Prasetyo, A. 2004. Pengaruh penambahan asam monokhloroasetat dan suhu pada pembentukan karboksimetil kitosan yang dihasilkan dari kitin yang berasal dari cangkang rajungan (Portunus pelagicus). Skripsi. Fakultas Farmasi, Universitas Pancasila. Jakarta. 81 p.

Rinaudo, M., P. Le Dung, and M. Milas. 1992. A new and simple method of synthesis of carboxymethylchitosans. In: Advances in chitin and chitosan. C.J. Brine, P.A. Sandford, and J.P. Zikakis. (Eds.). Elsevier Appl. Sci. Crown House, Essex, England. 516-523.

St. Angelo, A.J. and J.R. Vercellotti. 1989. Inhibition of warmed-over flavour and preserving of uncured meat containing materials. US Patents. 4,871,556.

Thatte, M.R. 2004. Synthesis and antibacterial assessment of water-soluble hydrophobic chitosan derivatives bearing quaternary ammonium functionality. Dissertation. Faculty of Louisiana State University and Agricultural Mechanical College. Department of Chemistry. Baton Rouge – Los Angeles. 107p.

Van der Lubben, I.M., J.C. Verhoef, G. Borchard, and H.E. Junginger. 2001. Chitosan and its derivatives in mucosal drug and vaccine delivery. Review. European Journal of Pharmaceutical Sciences. 14: 201-207.

Wibowo S., V. Savant, and J.A. Torres. 2003. Surimi wash water treatment by chitosan-alginate complexes: effect of chitosan concentration and treatment time on protein adsorption from surimi wash water. PhD dissertation. Corvallis, OR: Oregon State Univ. 123 p.

Wuriyandari, Y. 2002. Pengembangan turunan kitosan larut air. Skripsi. Fakultas Matematika dan Ilmu Pengetahuan Alam. Universitas Negeri Jakarta. Jakarta.16 p.

Xie, W., P. Xu, and Q. Liu. 2001. Antioxidant activity of water-soluble chitosan derivatives. Bioorganic & Biomedicinal Chemistry Letters. 11: 1699-1701.

Zhai, M., F. Yoshii, and T. Kume. 2003. Studying on antibacterial starch/chitosan blend film formed under the action of irradiation. J. Carbohydrate Polymer. 52(3): 311-317.



DOI: https://doi.org/10.22146/jfs.8929

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Jurnal Perikanan Universitas Gadjah Mada (print ISSN 0853-6384; online ISSN 2502-5066) is published by Department of Fisheries, Universitas Gadjah Mada in collaboration with Semnaskan UGM (Seminar Nasional Tahunan Hasil Perikanan dan Kelautan) and ISMFR (International Symposium on Marine and Fisheries Research).

 

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