EFEKTIVITAS EKSTRAK BAWANG PUTIH (Allium sativum) UNTUK MENCEGAH DAN MENGOBATI MOTILE AEROMONAS SEPTICEMIA (MAS) PADA IKAN MAS (Cyprinus carpio)

https://doi.org/10.22146/jfs.8921

Iesje Lukistyowati(1*), Windarti Windarti(2), Morina Morina(3), Alim Isnansetyo(4), Kurniasih Kurniasih(5)

(1) Fakultas Perikanan dan Ilmu Kelautan Universitas Riau
(2) Fakultas Perikanan dan Ilmu Kelautan Universitas Riau
(3) Fakultas Perikanan dan Ilmu Kelautan Universitas Riau
(4) Jurusan Perikanan Fakultas Pertanian Universitas Gadjah Mada
(5) Fakultas Kedokteran Hewan Universitas Gadjah Mada
(*) Corresponding Author

Abstract


The aim of study was to know the effectiveness of garlic (Allium sativum) extract in preventing and curing Motile Aeromonas Septicemia (MAS) in common carp (Cyprinus carpio). Garlic extract was obtained by juicing fresh garlic and filtering by using filter paper. Common carp (8-12 cm in total lenght) were used. Infection was done by injecting intraperitonially 0.1 ml 0f 24 h Aeromonas hydrophila culture at bacterial density of 108 cells/ml. Garlic extract was given by immersing the fish at various concentrations. Results indicated that the minimum inhibitory concentration (MIC) of the garlic extract againtst A. hydrophila ranged from 61.300-125.000 ppm, while the LD50 of this extract was 3.438 ppm. The use of garlic extract to prevent MAS was effective as survival rate of the treated fish with the extract at 1000, 2000, and 3000 ppm ranged from 83 to 100% while the survival rate of untreated fish was 60.71%. However, the use of garlic extract to cure MAS was not effective as the survival rate of the treated fish ranged 50-80% which were the same or even lower than that of the control (76.67%).


Keywords


Aeromonas hydrophila, common carp (Cyprinus carpio), garlic extract, MAS disease prevention

Full Text:

PDF


References

Agarwal, K.C. 1996. Therapeutic actions of garlic constituents. Med. Res. Rev. 16:111-114.

Anderson, D.P. 1974. Diseases of fish. T.F.H.Publication. Inc. Hongkong. 239 p.

Challem, J. 1995. The wonders of garlic. http://www.thenutritionreporter.com. Dikses tanggal 27 Oktober 2004.

Fulder, S., J. Blackwood, dan E. Soestrisno. 2000. Terapi bawang putih obat asli alami. Inovasi. Jakarta. 115 p.

Horikawa, M., T. Noro, and Y. Kamei. 1999. In vitro anti-methicillin resistant Staphylococcus aureus activity found in extract of marina algae indigenous to the coastline of Japan. J. Antibiotics. 52:186-189

Isnansetyo, A. and Y. Kamei. 2005. Direct antagonistic Method for screening anti-methicillin resistant Staphylococcus aureus (MRSA) substance, producing marine bacteria. Biota. 10 (3): 141-145.

Liu, B.M.D. 2005. Terapi bawang putih. Prestasi Pustaka. Jakarta. 243 p.

Lukistyowati, I. 2003. Sensitivitas bakteri Aeromonas hydrophila terhadap bawang putih (Allium sativum). Laporan Penelitian Lembaga Penelitian Universitas Riau. 39 p.

Lukistyowati, I. 2005. Pemanfaatan ekstrak bawang putih (Allium sativum) untuk pengobatan penyakit bakteri Aeromonas hydrophila pada ikan mas (Cyprinus carpio L). Jurnal Terubuk. 32 (1): 1-9.

Mariyono, A. Puspitasari, dan Sutomo. 2000. Pengaruh ekstrak bawang putih terhadap kelangsungan hidup benih ikan lele dumbo yang diinfeksi Aeromonas hydrophila.

Martin, K.W. and E. Ernst. 2003. Herbal medicines for treatment of bacterial infections: a review of controlled clinical trials. J. Antimicrob. Chemoter. 51 (2): 241-246.

Miya, T.S., Holck, H.G.O., Yim, G.K.W., Mennear, J.H.; Spratto, G.R. 1976. Laboratory guide in pharmacology. Fourth Edition. Burgess Publishing Company. Minneapolis, Minnesota 55435. hal 133 – 134.

Nagawa, R., N. Iwata, K. Ishikawa, H. Fukuda, T. Fujino, and A. Suzuki. 1996. Inhibition of microbial growth by ajoene, sulfur-containing compound derived from garlic. Journal Applied and Environmental Microbiology. 62: 4238-4243.

Salman, H., M. Bergman, H. Bessler, I. Punsky and M.Djaldetti. 1999. Effect of a garlic derivative (alliin) on peripheral blood cell immune responses. Int. J. Immuno-pharmacol. 21 : 589 -97

Sharman, V., M. Sethi, A. Kumar, and J. Rarotra. 1977. Antibacterial property of Allium sativum Linn: in vivo and in vitro studies. Ind. J. Exp. Biol. 15: 466-468.

Sivam, G.P. 2001. Protection against Helicobacter pyloric and other bacterial infections by garlic. Journal of Nutritrion. 131:1106S-1108S.

Syamsiah, I.S. dan Tajudin. 2003. Khasiat dan manfaat bawang putih raja antibiotik alami. Agromedia Pustaka. 63 p.

Triyanto. 1990. Patogenisitas beberapa isolat Aeromonas hydrophila terhadap ikan lele (Clarias batrachus L). Prosiding Seminar Nasional II Penyakit Ikan dan Udang. Balai Penelitian Perikanan Air Tawar. Pusat Penelitian dan Pengembangan Perikanan. Badan Penelitian dan Pengembangan Pertanian: 116-122.

Watanabe, T. 2001. Garlic therapy (Penyembuhan dengan terapi bawang putih diterjemahkan oleh Sumintadiredja). Penerbit PT Gramedia Pustaka Utama. Jakarta. 103 p.

Wibowo, S. 1989. Budidaya bawang putih, bawang merah, dan bawang bombay. Penebar Swadaya. Jakarta 194 p.

Zar, G.H. 1999. Biostatistical analysis. 4th Edition. Prentice Hall. London. 663 p.



DOI: https://doi.org/10.22146/jfs.8921

Article Metrics

Abstract views : 10064 | views : 11038

Refbacks

  • There are currently no refbacks.




Copyright (c) 2016 Journal of Fisheries Sciences



Jurnal Perikanan Universitas Gadjah Mada (print ISSN 0853-6384; online ISSN 2502-5066) is published by Department of Fisheries, Universitas Gadjah Mada in collaboration with Semnaskan UGM (Seminar Nasional Tahunan Hasil Perikanan dan Kelautan) and ISMFR (International Symposium on Marine and Fisheries Research).

 

View My Stats