* Corresponding Author
PENINGKATAN IMUNITAS BENIH IKAN KERAPU LUMPUR, Epinephelus coioides TERHADAP INFEKSI VIRUS IRIDO DENGAN APLIKASI VITAMIN C DAN BAKTERIN
https://doi.org/10.22146/jfs.8881 Fris Johnny(1*), Des Roza(2), Agus Priyono(3)
(1) Balai Besar Riset Perikanan Budidaya Laut Gondol
(2) Balai Besar Riset Perikanan Budidaya Laut Gondol
(3) Balai Besar Riset Perikanan Budidaya Laut Gondol
(*) Corresponding Author
Abstract
An experiment to increase of immune system of mangrove grouper, Epinephelus coioides seed with application of vitamin C and immunostimulant have been done. The experiment was carried out in completely randomized design with three treatments in twoplicates. The mangrove grouper seed (5 cm total length) were treated with 1000 mg vitamin C/kg diet (A), 1000 mg vitamin C + 1 ml bacterin/kg diet (B), and control (C). Vitamin C and bacterin were given every 10 day during 60 days rearing. After 30 and 60 days fishes were challenged with irridovirus. At the end of experiment, the non-specific immune responses were examined. The result showed that at challange test I, the highest survival rate was at treatment B (56.6%), followed by A (55.0%) and control (41.7%). On challenge test II, the higest survival rate was at treatment B (76.7%) followed by treatment A (68.4%) and control (48.3%). The highest phagocytic activity (PA) was obtained at treatment B (21.0%), followed by treatment A (18.5%) and control (9.5%). The highest of lysozyme activity (LA) value was obtained on treatment B (2.0 cm), followed by treatment A (1.9 cm) and control (1.3 cm). Combination of addition of ascorbic acid and immunostimulant gave higher survival rate compared to addition of immunostimulant only and control. Non-spesific immune response of mangrove grouper fry was significant different among treatment (P<0,05).
Keywords
Full Text:
PDFReferences
Anderson, D.P. 1996. Environmental factors in fish health: immunological aspects. In The fish immune system: organism, pathogen and environment. G. Iwama and T. Nakanishi (eds.), p. 289-310. Academic Press. USA.
Arimoto, M., K. Mori, T. Nakai, K. Muroga and I. Furusawa. 1993. Pathogenicity of the causative agent of viral nervous necrosis disease in striped jack, Pseudocaranx dentex (Bloch and Schneider). Journal of Fish Disease. 16:461 – 469.
Chou, H. Y., C. C. Hsu and T. Y. Peng. 1998. Isolation characterization of a pathogenic iridovirus from cultured grouper (Epinephelus sp.) in Taiwan. Journal of Fish Patholology, 33, 201-206.
Danayadol, Y., S. Direkbusarakom, S. Boonyaratpalin, T. Miyazaki and M. Miyata. 1997. Iridovirus infection in brown-spotted grouper (Epinephelus malabaricus) cultured in Thailand. In T. W. Flegel and I. H. MacRae (eds), Diseases in Asian Aquaculture III. Fish Health Section, Asian Fisheries Society, Manila. 67-72.
Ellis, A.E. 1993. Lysozyme assays. P. 101-103. In Stolen et al. (Eds.). Techniques in fish immunology-1. Sos Publications, Fair Haven, NJ 07760. USA.
Halver, J.E. 1989. The vitamin (In) Halver, J.E. (Eds) Fish nutrition; Second Edition. Academic Press, Inc. San Diego, California. P.32-101.
Itami, T., M. Kando, M. Uozu, A. Suganuma, T. Abe, A. Nakagawa, N. Suzuki and Y. Takahashi. 1996. Enhancement of resistance against Enterococcus seriolicida infection in yellowtail, Seriola quinqueradiata (Temminck & Schlegel), by oral administration of peptidoglycan derived from Bifidobacterium thermophylum. Journal of Fish Disease. 19:185-187.
Johnny, F., Koesharyani, I., Roza, D., Tridjoko, Giri, N.A. dan Suwirya, K. 2001. Respon ikan kerapu bebek, Cromileptes altivelis terhadap imunostimulan peptidoglycan melalui pakan pelet. Jurnal Penelitian Perikanan Indonesia. Vol. VII. No. 4, p. 52-56.
Johnny, F., Zafran, D. Roza dan I. N. A. Giri. 2002. Pengaruh vitamin C dalam pakan terhadap perubahan hemositologi ikan kerapu bebek, Cromileptes altivelis. Jurnal Aquaculture Indonesia. 3(1):27-34.
Johnny, F. dan D. Roza. 2004. Pengaruh penyuntikan imunostimulan peptidoglikan terhadap peningkatan tanggap kebal non-spesifik ikan kerapu macan, Epinephelus fuscoguttatus. Jurnal Aquaculture Indonesia. 5(2): 109-115.
Johnny, F., D. Roza, K. Mahardika, Zafran dan A. Prijono. 2005. Penggunaan imunostimulan untuk meningkatkan kekebalan non-spesifik benih ikan kerapu lumpur, Epinephelus coioides terhadap infeksi virus irido. Jurnal Penelitian Perikanan Indonesia. Vol. 11(5):75-84.
Klontz, G.W. 1997. Fish Hematology. In : Techniques in fish immunology-3 (2nd ed). Stolen, J.S., T.C. Fletcher, A.F. Rowley, J.T. Zelikoff, S.L. Kaattari, S.A. Smith. (Eds.). SOS Publications, Fair Haven, NJ 07704-3303. USA. p.121-131
Kurita, J., K. Nakajima, I. Hirono and T. Aoki. 1998. Polymerase chain reaction (PCR) amplification of DNA of red sea bream iridovirus (RSIV). Journal of Fish Pathology, 33, 17-23.
Lagler, K.F., J.E. Bardach, R.R. Miller and D.R.M. Passino. 1977. Ichtiology. John Willey and Sons. Inc. New York-London. 506 pp.
Mahardika, K., Zafran, D, Roza dan F. Johnny. 2004. Uji kerentanan ikan kerapu Lumpur, Epinephelus coioides dan kerapu batik, Epinephelus microdon terhadap infeksi iridovirus. Jurnal Penelitian Perikanan Indonesia. Vol. 10 (2) : 83-88.
Rowley, A.F. 1993. Collection, separation and identification of fish leukocytes. P. 113-136. In Stolen et al. (Eds.). Techniques in Fish Immunology-1. Sos Publications, Fair Haven, NJ 07760. USA.
Santarem,M., B. Novoa and A. Figueras. 1997. Effect of -Glucan on the non-specific immune responses of turbot (Schopthalamus maximus L.). Journal of Fish and sellfish Immunology, 7:429-437.
Secombes, C.J. 1996. The nonspecific immune system; cellular defences. In : The fish immune system: organism, pathogen and environment. G. Iwama and T. Nakanishi (Eds.). Academic Press. San Diego, California. p. 63-95
Siwicki, A.K. and Anderson, D.P. 1993. Immunostimulation in fish : Measures the effects of stimulants by serological and immunological methods, International Workshop and Training Course in Poland. 15 p.
Vadstein, O. 1997. The use of immunostimulatin in marine larviculture: possibilities and challenges. Journal of Aquaculture, 155:401-417.
Verlhac, V., Alex Obach., Jacques Gabaudan., Willy Schuep., Reid Hole. 1998. Immunomodulation by dietary vitamin C and glucan in rainbow trout (Oncorhynchus mykiss). Journal of Aquaculture 8 : 409-424
Zafran, I. Koesharyani, D. Roza, F. Johnny dan K. Yuasa. 1998. Peningkatan sintasan ikan kerapu tikus (Cromileptes altivelis) dengan penambahan vitamin dan imunostimulan ke dalam pakan segar. Sudrajat, A., E.S. Heruwati, K. Sugama, A. Purnomo, Z.I. Azwar, I.N.A. Giri. (Eds.) Seminar Teknologi Perikanan Pantai. Denpasar 6-7 Agustus 1998. 164-168.
DOI: https://doi.org/10.22146/jfs.8881
Article Metrics
Abstract views : 2453
| 
views : 8314
Refbacks
- There are currently no refbacks.
Copyright (c) 2016 Journal of Fisheries Sciences
Jurnal Perikanan Universitas Gadjah Mada (print ISSN 0853-6384; online ISSN 2502-5066) is published by Department of Fisheries, Universitas Gadjah Mada in collaboration with Semnaskan UGM (Seminar Nasional Tahunan Hasil Perikanan dan Kelautan) and ISMFR (International Symposium on Marine and Fisheries Research).
View My Stats