Alim Isnansetyo(1*)

(1) Fakultas Pertanian UGM
(*) Corresponding Author


Motile Aeromonas Septicimia is a dangerous disease on fresh water fish especially walking catfish and carp. Vaccination against the disease has been developed in Indonesia. However, there were high result variations of the vaccination. Immune response is influenced by level of vitamin C dietery. Objective of this experiment was to know effect of vitamin c levels on walking catfish’s specific immune respose.


The levels of vitamin (0, 50, 500, and 1000 mg ascorbic acid (AA)/kg feed) were given to the fish. Immersion vaccination for 30 minutes (108 sel/ml) was carried out 15 days after feeding trial. Challenge test was conducted by injection of 0,2 ml bacteria suspension (LD80 concentration) was carried out 15 days after vaccination. Growth rate of the fish was observed monthly.


Result of this experiment indicated that mortality in the challenge test due to A. hydrophila infection considerably decreased for treatment combination among vaccination; and 500 and 1000 mg AA/kg feed. These AA levels without vaccination could not reduce mortality.

Full Text:



Ellis, A.E., 1978. The Immunology of teleosts. Fish Pathology. Robert, R.J. (Ed.). Bailliere Tindall. London. 92-104.

Ellis, A.E., 1998b. optimizing factor for fish vaccination. Fish vaccination. Ellis, A.E (Ed). Academic Press. London. 32-46

Hardie, A,e,; Fletcher, T.C. and Secombes, C.J., 1991. The effect of dietary vitamin C on the immune response of the atlantic salmon (Salmo salar L.). Aquaculture 95:201-214.

Hubert, J.J., 1980 . Bioassay. Kendall Hunt Publishing. Dubugue.

Lio-Po, G.D., Albright, L.J., and Alapide Tendencia, E.V., 1992. Aeromonas hydophila in the ulcerative syndrome (EUS) of snakehead, Ophiocephalus striatus, and catfish, clarias batrachus: Quantitaive estimation in natural infection and experimental induction of dermomuscular necroticlesion. Diseases in Asian Aquaculture. Shariff, M, Subasinghe, R.P. and Arthur, J.R. (Ed.). AFS. 461-474.

Masumoto, T.; Hosokawa, H. and shimeno, S,. 1991. Ascorbic acid’s role in aquaculture nutrition. Proc. Of the aquaculture feed processing and nutrition workshop. ASA. Singapore. 42-48.

O’Keefe, T., Grout, B.F., 1991. Stabell form of vitamin C, essensiality, stability and bioavailability. ASA Technical Bulletin (29); 12 pp.

Salati, F., 1988. Vaccination against Edwarsiella tarda. Fish vaccination. Ellis A.E (Ed). Academic Press. London. 135-151.

Setyabudi, A.M.A., Grant, B.F. and Halver Halver, J.E., 1990. Pengaruh L-Ascorbyl-2-Phospate (ASPP) pada pertumbuhan dan resistensi ikan rainbow trout (Onchorhyncus mykiss) terhadap Inection Hematoporetic Necrosis Virus (IHNV). Prosiding Seminar II Penyakit Ikan dan Udang. Balitbang Pertanian : 71-82.


Article Metrics

Abstract views : 1546 | views : 2523


  • There are currently no refbacks.

Copyright (c) 2016 Journal of Fisheries Sciences

Jurnal Perikanan Universitas Gadjah Mada (print ISSN 0853-6384; online ISSN 2502-5066) is published by Department of Fisheries, Universitas Gadjah Mada in collaboration with Semnaskan UGM (Seminar Nasional Tahunan Hasil Perikanan dan Kelautan) and ISMFR (International Symposium on Marine and Fisheries Research).


View My Stats