Variasi Genetik Ikan Hias Clown, Amphiprion ocellaris

https://doi.org/10.22146/jfs.62

Sari Budi Moria(1*), Ida Komang Wardana(2), Gusti Ngurah Permana(3), Ahmad Muzaki(4), Ketut Maha Setiawati(5)

(1) Balai Besar Riset Perikanan Budidaya Laut, Gondol
(2) Balai Besar Riset Perikanan Budidaya Laut, Gondol
(3) Balai Besar Riset Perikanan Budidaya Laut, Gondol
(4) Balai Besar Riset Perikanan Budidaya Laut, Gondol
(5) Balai Besar Riset Perikanan Budidaya Laut, Gondol
(*) Corresponding Author

Abstract


Evaluation of genetic variation of wild clown fish, Amphiprion ocellaris was conducted to collect basic knowledge in order to develop sustainable ornamental fish breeding under controled condition. Clown fish samples were collected from three locations, i.e. Bali, Madura, and South Sulawesi waters. PCR amplification of mt-DNA by using universal primer obtained single band with molecule weight of 461 bp. RFLP of mt-DNA with Hinf I, Mbo I and Nla III restriction enzymes showed that the highest genetic variation as 0.581 was found from South Sulawesi population and the lowest as 0.382 from Bali, while Madura population has genetic variation of 0.456. Genetic distance of clown fish from South Sulawesi and Madura was closer (0.107) comparing to clown fish from Bali population (0.270).

Keywords


Clown fish, Genetic variation, RFLP-mt-DNA

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References

Allen, G.P. 1972. The anemone fish. Their classification and biology. T.F.H. Publication, Inc. Ltd. 288 p.

Allendorf, F.W. and S.R. Phelps. 1980. Loss of genetic variation in hatchery stock of cut throat trout. Trans Am.Fish.Soc. 109 : 537-543.

Andamari, R., Haryanti, K. Suwirya, S.B. Moria dan G.N. Permana. 2004. Bioreproduksi dan karakteristik variasi genetik ikan kerapu sunu, Plectropomus leopardus. Laporan Teknis Proyek Riset Perikanan Budidaya Laut Gondol-Bali. Balai Besar Riset Perikanan Budidaya Laut Gondol-Bali. 12 p.

Imron, K. Sugama, K. Sumantadinata and K. Soewardi. 1999. Genetic variation in cultured stocks of Tiger Shrimp (Penaeus monodon) in Indonesia. Indonesian Fisheries Research Journal. V(1) :10-18.

Leary, F.L., F.W. Allendorf, and K.L. Knudsen. 1985. Developmental instability as an indicator of reduced genetic variation in Hatchery Trout. Trans. Am. Fish. Soc.114 : 230-235.

Moria, S.B., Haryanti, G.N. Permana, I.K. Wardana dan B. Slamet. 2005. Studi Pendahuluan karakter genetik ikan napoeon, Cheilinus undulatus dengan metode Restriction Fragment Length Polymorphism (RFLP) mt-DNA. Prosiding Seminar Nasional Hasil Penelitian Perikanan dan Kelautan Tahun 2005 tanggal 30 Juli. Yogyakarta : 48-54.

Nei’s, M. 1972. Genetic distance between populations. American Naturalis. 106 (949) : 283-292.

Ohtani,T., K. Miyahara and N. Shimamoto. 1997. Genetic variability of allozymes in japanase Sea Bass, Lateolabrax japonicus in the coastal water of Hyogo prefecture. Fisheries Science. 63 (5) : 731.

Ovenden,J. 2000. Development of restriction enzyme markers for Red Snapper (Lutjanus erythropterus and Lutjanus malabaricus) stock discrimination using genetic variation in mitochondria DNA. Molecular Fisheries Laboratory, Southern Fisheries Centre. 18 p.

Permana, G.N., S.B. Moria, Haryanti, and K. Sugama. 2003. Genetic identification and variation of Red Snapper, Lutjanus sp. through allozyme electrophoretic analysis. Indonesian Fisheries Research Journal. IX (1):33-40.

Poernomo,A., S. Mardlijah, M.L. Linting, E. M. Amin, dan Widjopriono. 2003. Ikan hias laut Indonesia. Penebar Swadaya. Jakarta. 182 p.

Sugama, K., Tridjoko, Haryanti, S.B. Moria and F. Cholik. 1998. Genetic variation and population structure in the Humback grouper, Cromileptes altivelis throughout its range in Indonesia waters. Indonesian Fisheries Research Journal. V (1) :32-38.

Taniguchi, N., K. Sumantadinata and S. Iyama. 1983. Genetic change in the first and second generation of hatchery stock of black sea bream. Aquaculture. 35:309-320.



DOI: https://doi.org/10.22146/jfs.62

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Jurnal Perikanan Universitas Gadjah Mada (print ISSN 0853-6384; online ISSN 2502-5066) is published by Department of Fisheries, Universitas Gadjah Mada in collaboration with Semnaskan UGM (Seminar Nasional Tahunan Hasil Perikanan dan Kelautan) and ISMFR (International Symposium on Marine and Fisheries Research).

 

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