Pembuatan Konsentrat Omega-3 dari Minyak Hati Hiu Botol dengan Metode Kristalisasi Urea

https://doi.org/10.22146/jfs.60352

Anies Chamidah(1*), Andaru Wicaksono(2)

(1) Teknologi Hasil Perikanan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Brawijaya, Malang, Jawa Timur, Indonesia
(2) Alumni Fakultas Perikanan dan Ilmu Kelautan, Universitas Brawijaya, Malang, Jawa Timur, Indonesia
(*) Corresponding Author

Abstract


Minyak ikan adalah sumber terbaik dari omega-3 khususnya EPA dan DHA yang penting untuk kesehatan manusia. Hati hiu kaya akan minyak, sampai saat ini, pemanfaataannya terbatas sebagai sumber squalene atau bahkan dibuang sebagai limbah padahal kandungan asam lemak omega-3nya masih cukup tinggi. Tujuan penelitian ini adalah pembuatan konsentrat omega-3 dari minyak hati ikan hiu. Metode yang digunakan adalah kompleks urea yang dapat memisahkan antara asam lemak jenuh dan tak jenuh dengan perlakuam Aa (rasio urea : asam lemak = 2 :1 suhu 25°C), Ab (rasio urea : asam lemak = 2 :1 suhu 5°C), Ba (rasio urea : asam lemak = 4:1 suhu 25°C) dan Bb (rasio urea : asam lemak = 4 :1 suhu 5°C). Perlakuan terbaik diperoleh pada perlakuan rasio urea: minyak ikan (4:1) dengan suhu pengadukan 5°C (Ba) menghasilkan rendemen sebesar 28,78% dan bilangan iod sebesar 182,48 g/100g serta kadar EPA sebesar 20,04% dan  DHA sebesar 13,59% dengan total asam lemak PUFA sebesar 35,78%. Kesimpulannya pembuatan konsentrat omega-3 dari minyak hati hiu botol dengan metode kristalisasi urea layak diupayakan.

Keywords


EPA; DHA; Konsentrat Omega-3; kristalisasi urea; minyak hati hiu botol

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References

Aisyah, S., Y. Yulianti. & A.G. Fasya. 2010. Penurunan angka peroksida dan asam lemak bebas (FFA) pada proses bleaching minyak goreng bekas oleh karbon aktif

polong buah kelor (Moringa oliefera. Lamk) dengan aktivasi NaCl. Alchemy. 1 (2): 93-103.

Budiadnyani, I.G.A. 2017. Pengaruh Jenis dan konsentrasi adsorben terhadap karakteristik fisikokimia minyak ikan dari hasil samping industri pengalengan tuna madidihang (Thunnus Albacares). Technology Science and Engineering Journal. 1 (2): 119-127.

Chakraborty, K., P. Vijayagopal, R.D. Chakraborty & K.K. Vijayan. 2010. Preparation of eicosapentaenoic acid concentrates from sardine oil by Bacillus circulans lipase. Food Chemistry. 120: 433-442.

Damongilala L.J. 2008. Kandungan asam lemak tak jenuh minyak hati ikan cucut botol (Centrophorus sp.) yang diekstraksi dengan cara pemanasan. Jurnal Ilmiah Sains. 2 (8): 249-253.

Dovale-Rosabal, G., A. Rodríguez, E. Contreras, J. Ortiz-Viedma, M. Muñoz, M. Trigo, S.P. Aubourg & A. Espinosa. 2019. Concentration of EPA and DHA from refined salmon oil by optimizing the urea–fatty acid adduction reaction conditions using response surface methodology. Molecules. 24: 1642. doi:10.3390/molecules 24091642

Efendi, S.C., A.D. Anggo & W. Wijayani. 2020. Pengaruh suhu ekstraksi pada metode dry rendering terhadap kualitas minyak kasar hati ikan manyung (Arius thalassinus). Jurnal Ilmu dan Teknologi Perikanan. 2 (1).

Estiasih, T. 2006. Kristalisasi urea pada pembuatan konsentrat asam lemak w-3: Kajian pustaka. Jurnal Teknologi Pertanian. 7 (1): 61-70.

Fuadi, I. & S.H. Suseno. 2014. Characterization of fish oil from mackerel (Scomber japonicus) canning by product. Asian Journal of Agriculture and Food Science. 2 (3).

García, C.M., M. Fernández & M. Castiñeira. 2014. Evaluation and establishment of the quality specifications of the shark liver oil pool. Ars Pharm. 5 (35): 23-29.

Gupta, P., K. Singhal, A.K. Jangra, V. Nautiyal & A. Pandey. 2012. Shark liver oil: A review. Asia Journal of Pharmaceutical Education and Research. 1 (2): 1-15.

Ibrahim, B., P. Suptijah & G. Yogaswara. 2016. Karakterisasi minyak ikan dari hasil samping industri penepungan ikan lemuru (Sardinella lemuru) dengan metode pemurnian alkali. Dinamika Maritim. 5 (1): 1-7.

Jimenez, D. 2021. Covid-19 vaccines: exploring animalfree alternatives to shark squalene. Pharmaceutical Technology. https://www.pharmaceutical-technology.com/features/covid-19-vaccines-animal-free-alternatives-sharksqualene/.

Jumari, A., A.S. Rahmani & F.R. Riana. 2015. Fraksinasi kompleksasi urea pada minyak dedak padi dalam peningkatan konsentrasi asam lemak tak jenuh. Ekuilibrium. 14 (1): 17-22.

Kapoor, R. & U.K. Patil. 2011. Importance and production of omega-3 fatty acids from natural sources. International Food Research Journal. 18: 493-499.

Lamas, D.L. & A.E. Massa. 2019. Ray liver oils obtained by different methodologies: Characterization and refining. Journal of Aquatic Food Product Technology. 28 (5): 555-569 DOI: 10.1080/ 10498850. 2019.160555.

Lubis, M.R. & M. Nova. 2013. Leaching of oil from tuna fish liver by using solvent of methyl-ethyl ketone. Jurnal Rekayasa Kimia dan Lingkungan. 9 (4): 188-196.

Ma, Y.J. & B. Yang. 2010. World Marine fish resources utilization situation and development trend. Chin Oil. 35: 1-3.

Maulana, I., S. Sukrasno & S. Damayanti. 2020. Recycling of “minyak ala muncar” by three crystallization methods. Authorea. DOI:10.22541/au.159164060.02821169.

Mbatia. B., P. Adlercreutz, F. Mulaa & B. Mattiasson. 2010. Enzymatic enrichment of omega-3 polyunsaturated fatty acids in nile perch (Lates niloticus) viscera oil. European Journal of Lipid Science and Technology. 112: 977-984.

Montesqrit, M & R. Ovianti. 2013. Pengaruh suhu dan lama penyimpanan terhadap stabilitas minyak ikan dan mikrokapsul minyak ikan. Jurnal Peternakan Indonesia. 15 (1).

Mustikawati, L. 1998. Mikroenkapsulasi konsentrat asam lemak omega-3 dari minyak limbah pengalengan lemuru (Sardinella lemuru) dengan koaservasi kompleks. Jur. Teknologi Pangan dan Gizi. FTP. IPB. Bogor.

Navarro, G., R. Pacheco, B. Vallejo, J. Ramirez & A. Bolaños. 2000. Lipid composition of the liver oil of shark species from the Caribbean and Gulf of California waters. Journal of Food Composition and Analysis. 13: 791-798.

Patil, D & A. Nag. 2011. Production of PUFA concentrates from poultry and fish processing waste. Journal of the American Oil Chemists’ Society. 88: 589-593.

Patil, D. 2014. Recent trends in production of polyunsaturated fatty acids (PUFA) Concentrates. Journal of Food Research and Technology. 2: 15-23.

Peixoto, J.V.C., L.M.R. de-Paula, F. Iagher, I.K. Silva, F.A.L. Dias & R.T.H. Fogaça. 2020. Shark liver oil consumption decreases contractility in EDL muscle of trained rats. Fisioter. Mov., Curitiba, v. 33, e003311, 2020. ISSN 0103-5150. DOI: http://dx.doi.org/10.1590/1980-5918. 033.AO11

Poudyal, H., S.K. Panchal, V. Diwan & L. Brown. 2011. Omega-3 fatty acids and metabolic syndrome : effects and emerging mechanisms of action. Progress in Lipid Research. 50: 372-387.

Sellami, M., F. Rebah, Y. Gargouri & N. Miled. 2018. Lipid composition and antioxidant activity of liver oils from ray species living in Tunisian coasts. Arabian J. Chem. 11: 233–239. doi:10.1016/j.arabjc.2014.07.010.

Sudarmadji, S., B. Kartika & S. Suhardi. 2007. Analisis Bahan Makanan dan Pertanian. Liberty. Yogyakarta .

Suriani, N.W., H.J. Lawalata & A. Komansilan. 2014. Urea crystallization on the concentrate making of omega-3 fatty acid from oil of tuna fish (Thunnus sp.) canning byproduct. International Journal of PharmTech.

Suriani, N.W & A. Komansilan. 2019. Enrichment of omega-3 fatty acids, waste oil byproducts canning tuna (Thunnus sp.) with urea crystallization. Journal of Physics: Conference Series. (1317) 012056 IOP Publishing doi:10.1088/17426596/1317/1/012056.

Thammapat, P., S. Siriamornpun & P. Raviyan. 2016. Concentration of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) of Asian catfish oil by urea complexation: optimization of reaction conditions. Songklanakarin J. Sci. Technol. 38 (2): 163-170.

Ulilalbab, A & T. Estiasih. 2012. Optimasi sintesis fosfolipid terstruktur tinggi epa oleh lipase Rhizomucor miehei antara konsentrat asam lemak omega-3 dari minyak hasil samping penepungan lemuru dan fosfolipid kedelai varietas anjasmoro.

Undjung D. 2005. Continous production of pure squalene by using column chromatography. Indo. J. Chem. 5 (3): 251-254.

Venugopal, V., A.K. Kumaran, N.S. Chatterjee, S. Kumar, S. Kavilakath & J.R. Nair. 2016. Biochemical characterization of liver oil of Echinorhinus brucus (bramble shark) and its cytotoxic evaluation on neuroblastoma cell lines (SHSY-5Y). Scientifica. 6294030.

Wanasundara, U.N & f. Shahidi. 1999. Concentration of omega-3 polyun-saturated fatty acids of seal bubbler oil by urea complexation: Optimization of reactions conditions. Food Chemistry. 65: 41-49.

Wetherbee, B.M & P.D. Nichols. 2000. Lipid composition of the liver oil of deep-sea sharks from the Chatam Rise, New Zealand. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 125 (4): 511521.

Yığın, C.Ç., F. Çakır, K. Cabbar, B. Kızılkaya, H.B. Ormancı, A Öztekin & Y. Özüdoğru. 2019. The liver lipid fatty acid composition of two carti-laginous fish, the thornback ray (Raja clavata) and the common smooth-hound (Mustelus mustelus). Aquatic Research. 2 (3): 143-153. https://doi.org/10.3153/AR19012.

Zhang, C., M. Chen, Z. Mao & G. Zu. 2012. Concentration of DHA and EPA from marine fish oil by urea complexation. Advanced Materials Research. 581: 54-57.



DOI: https://doi.org/10.22146/jfs.60352

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