Protein Aeromonas Hydrophila Sebagai Vaksin untuk Pengendalian Mas (Motile Aeromonas Septicemia) pada Jambal Siam (Pangasius hypophthalamus)

https://doi.org/10.22146/jfs.59

Olga Olga(1*), Ririen Kartika Rini(2), Junius Akbar(3), Alim Isnansetyo(4), Langkah Sembiring(5)

(1) Jurusan Budidaya Perairan, Fakultas Perikanan Universitas Lambung Mangkurat
(2) Jurusan Budidaya Perairan, Fakultas Perikanan Universitas Lambung Mangkurat
(3) Jurusan Budidaya Perairan, Fakultas Perikanan Universitas Lambung Mangkurat
(4) Jurusan Perikanan, Fakultas Pertanian Universitas Gadjah Mada
(5) Jurusan Biologi Fakultas Biologi UGM
(*) Corresponding Author

Abstract


Objectives of this study were to find out the protein of Aeromonas hydrophila for vaccine, and to evaluate the efficacy of the protein to control Motile Aeromonas Septicemia (MAS) in catfish (Pangasius hypophthalamus). A. hydrophila was isolated from the kidney of diseased catfish. The isolate was cultured, sonicated and sentrifuged into cell-free extract and debris. The protein in the cell-free extract was precipitated with ammonium sulphate, dialized, then fractionated by gel filtration column chromatography on Sephadex G150.

The concentration of protein were estimated by spectrophotometer at 280 nm. Fractions obtained from the gel filtration with high concentration of protein were analized by sodium dodocyl sulphate-polyachrylamide gel electrophoresis (SDS-PAGE). The protein bands obtained from SDS-PAGE with molecular weight more than 10 kDa were tested for immunogenicity to mice. Futhermore, the most immunogenic protein was used for fish vaccination. The vaccinated and unvaccinated fishes were chalenged with A. hydrophila.

The results indicated that vaccination with the protein significantly increased (P<0.01) the antibody titer either in mice or catfish. Relative Percent Survival of catfish vaccinated with the protein at 5; 7.5; and 10 µg/fish were 61.54%, 80.77% and 76.92%, respectively. The optimum dose of vaccine was 8.33 µg/fish with maximum RPS of 82.05%.


Keywords


Aeromonas hydrophila, catfish, MAS, vaccine

Full Text:

Full Article (PDF)


References

Almendras, J.M.E. 2001. Immunity and biological methods of disease prevention and control. In: Health management in aquaculture. G.D. Lio-po, C.R. Lavilla, and E.R. Cruz-Lacierda (Eds.). Aquaculture Departement Southeast Asian Fisheries Development Center, Philippines: 111-136.

Anderson, D.P. 1974. Fish immunology. In: Disease of fishes. Volume 4. S.F. Snieszko and H.R. Axelrod (Eds.). T.F.H. Publication. Inc Ltd. The British Crown Colony of Hongkong. 239 p.

Bellanti, J.A. 1993. Immunobiology (Imunologi, diterjemahkan oleh A.S. Wahab). Gadjah Mada University Press. Fakultas Kedokteran UGM: 203-209.

Dorson, M. 1984. Applied immunology of fish. Symposium on Fish Vaccination. O.I.E. Fish Diseases Commission. Paris: 39-74.

Ellis, A.E. 1988. Optimizing factors for fish vaccination. In: Fish vaccination. A.E. Ellis (Ed.). Academic Press Ltd. London: 32-46.

Golub, E.S. 1987. Immunology a synthesis. Sinauer Associates, Inc. Sunderland-Massachusetts. 551 p.

Jutono, J. Soedarsono, S. Hartadi, S. Kabirun S., D. Suhadi, dan Soesanto. 1980. Pedoman praktikum mikrobiologi umum (untuk perguruan tinggi). Departemen Mikrobiologi Fakultas Pertanian Universitas Gadjah Mada. Yogyakarta. 181 p.

Murtiningsih. 2003. Penggunaan vaksin protein sitoplasma bakteri A. hydrophila pada lele dumbo (Clarias gariepinus). Skripsi. Fakultas Pertanian Jurusan Perikanan. Universitas Gadjah Mada. 74 p.

Nitimulyo, K.H. dan Triyanto. 1990. Sistem pertahanan tubuh dan diagnosis serologi penyakit ikan. Pelatihan Karantina Ikan. Bogor 21 Mei-4 Agustus 1990: 52-59.

Nitimulyo, K.H., Triyanto, dan Sri Hartati. 1994. Penanggulangan penyakit Motil Aeromonas Septicemia (MAS) pada ikan lele (Clarias sp.). Lembaga Penelitian UGM dan Balitbang Pertanian. Yogyakarta. 45 p.

Nitimulyo, K. H., Triyanto, dan Sri Hartati. 1997. Efikasi vaksin dan kemangkusan tetrasiklin untuk penanggulangan penyakit MAS pada pendederan lele dumbo (Clarias gariepinus). Jurnal Perikanan UGM (GMU Journal Fisheries Science). I (2): 25-30.

Olga, L. Sembiring, dan Triyanto. 2004. Pengendalian penyakit MAS (Motile Aeromonas Septicemia) pada lele dumbo (Clarias gariepinus) melalui vaksinasi. Sains dan Sibernatika. 17(3):467-476

Olga dan R.K. Rini. 2006. Penggunaan vaksin whole cell untuk pengendalian penyakit MAS (Motile Aeromonas Septicaemia) pada ikan patin (Pangasius hypophthalamus). Agroscientiae. 13 (1): 48-54

Pasaribu, F. H., N. Dalimunthe, dan M. Poeloengan. 1990. Pengobatan dan pencegahan penyakit ikan bercak merah. Prosiding Seminar Nasional II Penyakit Ikan dan Udang 16–18 Januari. Badan Penelitian dan Pengembangan Pertanian: 143-152.

Souter, B.W. 1984. Immunization with vaccines. Departement of Fish an Oceans. Winnipeg. Mannitoba: 111-117.

Stevenson, R.M.W. 1988. Vaccination against Aeromonas hydrophila. In: Fish vaccination. A.E. Ellis (Ed.). Academic Press Ltd. London: 112-121.

Subowo. 1993. Imunobiologi. Penerbit Angkasa. Bandung. 233 p.

Supriyadi, H..M., O. Komarudin, dan J. Slembrouk. 1998. Preliminary study of the source of Aeromonas hydrophila infection on Pangasius hypophtalamus larvae. In: The biological diversity and aquaculture of Clariid and Pangasiid catfish in South-East Asia. M. Legendre and A. Pariselle, (Eds.). Proceedings of The Midterm Workshop of the “Catfish Asia Project”: 219-222.



DOI: https://doi.org/10.22146/jfs.59

Article Metrics

Abstract views : 1669 | views : 2206

Refbacks

  • There are currently no refbacks.




Copyright (c)



Jurnal Perikanan Universitas Gadjah Mada Indexed by:

 

 

View My Stats