Isolation, Characterization and Pathogenicity of Edwardsiella tarda a Causative Disease on Freshwater Fish in Yogyakarta

https://doi.org/10.22146/jfs.39920

Murwantoko Murwantoko(1*), Eka Diniarti(2), Triyanto Triyanto(3)

(1) Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada.
(2) Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada.
(3) Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada.
(*) Corresponding Author

Abstract


Edwarsiella tarda is a cosmopolitan bacterium and is a cause of Edwardsiellosis in various fish species. The bacterial infection causes large losses on aquaculture in Asia, especially Japan. This study was conducted to isolate and characterize E. tarda as causative disease in freshwater fishes, and to determine its pathogenicity to catfish (Pangasius sp.). Bacteria were isolated from kidney of diseased fishes on Tryptone Soya Agar medium. Identification was conducted based on morphological colonies, morphological cells and biochemical tests. Fulfillment of Koch Postulates was done by injecting bacteria intraperitoneally on 7-9 cm fishes at dose of 107 cfu/fish. Pathogenicity test was carried out by intraperitoneal injection at 104, 105, 106, and 107 cfu/fish to 7-9 cm-catfish (Pangasius sp.) and followed by observation of disease signs and mortality every six hours for 7 days. Pathogenicity was determined as Lethal Dosage (LD50) using Dragstedt Behrens method. In this research we have isolated three isolates E. tarda causing disease in fishes. The clinical signs of this disease were lose of pigmentation over the lession, swollen of stomach, haemorhage on fins , small cutaneous lesions, and necrotic on fins area. The LD50 of E. tarda isolate L2, L3, and N3 were 4.64 ± 0.35x105, 1.54 ± 0.07x105, and 1.13 ± 0.13x106 cfu/fish, respectively.

Keywords


Edwardsiella tarda; LD50; catfish; Pangasius; tilapia

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References

Austin, B & D.A. Austi. 1987. Bacterial Fish Pathogens: Disease in Farmed and Wild Fish. John Wiley and Sons. Chichester.

Bondad-Reantaso, M.G., R.P. Subasinghe., J.R. Arthur., K. Ogawa., S. Chinabut., R. Adlard., Z. Tan & M. Shariff. 2015. Disease and health management in Asian aquaculture. Vet Parasitol.132 (3-4): 249-72.

Cowan, S.T. 1974. Manual for the Identification of Medical Bacteria. Second edition. Cambridge University. Cambridge. 130p.

Holt, J.G., P.H.A. Sneath., J.T. Stanley., & S.T Williams. 1994. Bergey’s Manual of Determinative Bacteriology. 9th Ed. Williams & Wilkins. Baltimore

MacFaddin, J.F. 1980. Biochemical Test for Identification of Medical Bacteria. Second Ed. Williams & Wilkins. Baltimore.

Murwantoko., Rozi., I. Istiqomah & K.H. Nitimulyo. 2013. Isolasi, Karakterisasi, dan Patogenitas Bakteri Penyebab Penyakit pada Gurami (Osphronemus goramy) di Kabupaten Bantul. Jurnal Perikanan XV (2): 83-90

Rejeki, S., Triyanto & Murwantoko. 2016. Isolasi dan Identifikasi Bakteri Aeromonas sp. dari Lele Dumbo (Clarias sp.) di Kabupaten Ngawi. Jurnal Perikanan 18 (2): 55-60.

Narwiyani, S. 2011. Lethal Concentration 50% (LC50) Empat Isolat Edwardsiella tarda pada Ikan Ari Tawar di Indonesia. Jurnal Sain Veteriner. 28 (2): 51-54.

Narwiyani, S. & Kurniasih. 2011a. Phylogenetic Tree dari Empat Isolat Edwardsiella tarda di Indonesia. 16 (2): 348−353.

Narwiyani, S. & Kurniasih. 2011b. Perbandingan Patogenesitas, Edwardsiella tarda Pada Ikan Mas Koki (Carassius auratus) Dan Ikan Celebes Rainbow (Telmatherina celebensis). Jurnal Riset Akuakultur. 6 (2): 291-301

Plumb,J.A. 1993. Edwardsiella Septicaemia dalam Bacterial Diseases of Fish. V. Inglish R. J. Robert and N. R. Bromage. Blackwell Scientific Publication. London. 61-79.

Plumb, J.A & L.A Hanson. 2011. Health Maintenance and principal microbial diseases of cultured fishes. Wiley- Blackwell. Iowa

Pridgeon, J.W & P.H. Klesius. 2012. Major bacterial diseases in aquaculture and their vaccine development. CAB Reviews 7: 048



DOI: https://doi.org/10.22146/jfs.39920

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