The Application of Response Surface Methodology (RSM) on the Optimization of Catfish Bone Calcium Extraction

https://doi.org/10.22146/jfs.35663

Susana Endah Ratnawati(1*), Nurfitri Ekantari(2), Rizky Wana Pradipta(3), B L Paramita(4)

(1) Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada. Yogyakarta
(2) Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada. Yogyakarta
(3) Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada. Yogyakarta
(4) Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada. Yogyakarta
(*) Corresponding Author

Abstract


This study was designed to determine the optimum conditions on catfish bone calcium extraction through Response Surface Methodology (RSM) which used factorial design and 13 treatments. Tests were used to know the influence of independent variables (solvents concentrations and treatment time) on the calcium content of bone flour. As a result, linear models were used as response prediction. Maximum response was obtained by calcium extraction using 5% NaOH during 30 minutes or 11.64% HCl within 58 minutes. It is shown that calcium content were 15.74-17.46% with more than 87.5% accuracy level. Calcium extraction using 5% NaOH during 30 minutes produced flour which has Ca/P ratio 3:1, maximum yield and whiteness level. Low protein and moisture content might result in the long shelf life of catfish bone flour.

Keywords


HCl; calcium; NaOH; Response Surface Methodology; catfish bone

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References

Agustini, T.W., S.E. Ratnawati, B.A. Wibowo & J. Hutabarat. 2011. Pemanfaatan cangkang kerang simping (Amusium pleuronectes) sebagai sumber kalsium pada produk ekstrudat. J. Pengolahan Hasil Perikanan Indonesia 14 (2): 134-142

Apriyana, I. 2014. Pengaruh penambahan tepung kepala ikan lele (Clarias sp.) dalam pembuatan cilok terhadap kadar protein dan sifat organoleptiknya. Unnes J. of Public Health 3 (2): 1-9

Association of Official Agriculture Chemist (AOAC). 1995. Official Methods of Analysis on the Association of Official Agriculture Chemist.Washington. DC.

Chow, L.C. 2001. Solubility of Calcium Phospates. Ed: Octacalcium Phospate. Monogr Oral Sci. Basel. Karger 18: 94-111.

Ferazuma, Her., M.S. Anna., Amalia & Leily. 2011. Substitusi tepung kepala ikan lele dumbo (Clarias Gariepinus) untuk meningkatkan kandungan kalsium crackers. J. Gizi dan Pangan 6 (1): 18–27

Hemung, Bung Orn. 2013. Properties of tilapia bone powder and its calcium Bioavailability based on translutaminase assay. International Journal of Bioscience, Biochemistry and Bioinformatics 3 (4) : 306-309.

Kementerian Kelautan dan Perikanan (KKP). 2012. Statistik Perikanan Tangkap, Perikanan Budidaya dan Ekspor - Impor Setiap Provinsi seluruh Indonesia, 2003-2010. Pusat Data Statistik dan Informasi, Kementerian Kelautan dan Perikanan.178 hlm.

Klomklao, S., S. Benjakul & H. Kishimura. 2013. Functional properties and antioxidative activity of protein hydrolysates from toothed ponyfish muscle treated with viscera extract from hybrid catfish. International J. of Food Science and Technology 48 (7): 1483-1489

Kustiani, A., C.M. Kusharto & E. Damayanthi. 2017. Pengembangan crackers sumber protein dan mineral dengan penambahan tepung daun kelor (Moringa oleifera) dan tepung badan-kepala ikan lele dumbo (Clarias gariepinus). Nutri-sains 1 (1): 22-38

Lekahena, V., D.N. Faridah., R. Syarief & R. Peranginangin. 2014. Karakterisasi fisikokimia nanokalsium hasil ektraksi tulang ikan nila menggunakan larutan basa dan asam. J. Teknologi Pangan 25 (1): 57-64

Li, Yun-Fang., Wu-Yu., E.N. Hernandez & P.J. Roberto. 2013. Heat and drought stresson durum wheat: responses of genotypes, yield and quality parameters. J. of Cereal Science 57: 398 – 404

Mahmoud, N.S., A.E. Ghaly & F. Arab. 2007. Uncoventional approach for demineralisazation of deproteinized crustacean shells for chitin production. American J. of Biochemistry and Biotechnology 3 (1): 1-9

Montgomery, D.C. 2001. Design and analysis of experiments. New York (US): John Wiley & Sons, Inc.

Pusat Data Statistik dan Informasi. 2015. Kelautan dan Perikanan dalam Angka tahun 2015. 308 hlm.

Ratnawati, S.E., T.W. Agustini & J. Hutabarat. 2014. Penilaian hedonik dan perilaku konsumen terhadap snack yang difortifikasi tepung cangkang kerang simping (Amusium sp.). Jurnal Perikanan Universitas Gadjah Mada 15 (2): 88-103.

Sanaei, A.V., F. Mahmoodani., S.F. See., S.M. Yusop & A.S. Babji. 2013. Optimization of gelatin extraction and physico-chemical properties of catfish (Clarias gariepinus) bone gelatin. International Food Research Journal 20 (1): 424-430.

SNI 01-2354.2-2006. Cara uji kimia bagian 2: penentuan kadar air pada produk perikanan. Badan Standarisasi Nasional, Jakarta.

Suptijah, P., A. Jacoeb & N. Deviyanti. 2012. Karakteristik dan bioavailabilitas nanokalsium cangkang udang vannamei (Litopenaeus vannamei). J. akuatika 3 (1): 63-73.

Trilaksani, W, S. Ella & N. Muhammad. 2006. Pemanfaatan limbah tulang ikan tuna (Thunnus sp.) sebagai sumber kalsium dengan metode hidrolisis protein. Buletin Teknologi Hasil Perikanan 9 (2) : 34-45.

Yadav, B.K & V.K. Jindal. 2007.Changes in head rice yield and whiteness during milling of rough rice (Oriza sativa L.). J. of Food Engineering 86 : 113 - 121



DOI: https://doi.org/10.22146/jfs.35663

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Jurnal Perikanan Universitas Gadjah Mada (print ISSN 0853-6384; online ISSN 2502-5066) is published by Department of Fisheries, Universitas Gadjah Mada in collaboration with Semnaskan UGM (Seminar Nasional Tahunan Hasil Perikanan dan Kelautan) and ISMFR (International Symposium on Marine and Fisheries Research).

 

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