* Corresponding Author
Toxicity Test for Evaluating Food Safety of New Edible Seaweeds, Enteromorpha sp. and Laurencia sp.
https://doi.org/10.22146/jfs.34103 Alim Isnansetyo(1*), Indah Istiqomah(2), Retno Widaningroem(3), Triyanto Triyanto(4), Rina Anggraeni Safia(5), Riesa Yosita(6), Senny Helmiati(7)
(1) Departement of Fisheries, Faculty Agriculture, Universitas Gadjah Mada, Yogyakarta
(2) Departement of Fisheries, Faculty Agriculture, Universitas Gadjah Mada, Yogyakarta
(3) Departement of Fisheries, Faculty Agriculture, Universitas Gadjah Mada, Yogyakarta
(4) Departement of Fisheries, Faculty Agriculture, Universitas Gadjah Mada, Yogyakarta
(5) Departement of Fisheries, Faculty Agriculture, Universitas Gadjah Mada, Yogyakarta
(6) Departement of Fisheries, Faculty Agriculture, Universitas Gadjah Mada, Yogyakarta
(7) Departement of Fisheries, Faculty Agriculture, Universitas Gadjah Mada, Yogyakarta
(*) Corresponding Author
Abstract
Seaweeds are potential foodstuff and source of bioactive substances that are very useful in the food and pharmaceutical industries. The objective of this study was to evaluate toxicity of aqueous extracts of seaweeds by Brine shrimp lethality test (BLT) and cytotoxicity test. The seaweeds used in this study were Enteromorpha sp. and Laurencia sp. collected from the Gunungkidul coastal line. The cytotoxicity test was performed against a normal cells line of Vero cells and a cancer cells line of HeLa cells. The cells lines were cultured in M119 medium with addition of 10% FBS and incubated at 37°C in CO2 incubator until confluent. Cells were harvested and cultured in 96 microplate with an initial density of 2x104 cells / 100 ml / well. The cells lines were treated with seaweed extracts at 24 hours after incubation. After additional 24 h incubation, the cell proliferation was observed by MTT method (3- [4,5-dimethylthiazol-2-yl] -2,5- diphenyltetrazolium bromide.The results showed that the two aqueous extract were not toxic in the BLT test, and did not exhibited any cytotoxicity activity against HeLa and Vero cells lines. Concentration-dependent cell proliferation was also not found. This results indicated that Enteromorpha sp. and Laurencia sp. extracts were no cytotoxic and might be consumed safely. These overall results suggested that the two seaweeds might be developed to be a new foodstuff for humans.
Keywords
Full Text:
PDFReferences
Bahar, B., J.V. O’Doherty., T.J. Smyth & T. Sweeney. 2016. A comparison of the effects of an Ascophyllum nodosum ethanol extract and its molecular weight fractions on the inflammatory immune gene expression in-vitro and ex-vivo. Innovatie Food Science and Emerging Technologies 37: 276-285
Bouga, M & E. Combet. 2015. Emergence of seaweed and seaweed-containing foods in the UK: Focus on Labelling, Iodine Conten, Toxicity and Nutrition. Foods 4 (2): 240-253
Brown, E.M, P.J. Allsopp., P.J. Magee., C.I.R Gill., S. Nitecki., C.R. Strain & E.M. McSorley. 2014. Seaweed and human health. Nutr. Rev 72: 205-2016
Cofrades, S., L_Lopez., C. Bravo., S. R-Capillas., M.T. Bastida., F.Larrea & J-Colmenero. 2010. Nutritional and antioxidant properties of different brown and red Spanish edible seaweeds. Food Science and Technology International. DOI: 10.1177/1082013210367049
Fleurencel, J., C. Le Coeur., S. Mabeau., M. Maurice & A. Landrein. 1995. Comparison of different extractive procedures for proteins from the edible seaweeds Ulva rigida and Ulva rotundata. Journal of Applied Phycology 7: 577-582.
Hiqashi-Okaja, K., S. Otanib & Y. Okai. 1999. Potent suppressive effect of a Japanese edible seaweed, Enteromorpha prolifera (Sujiao-nori) on initiation and promotion phases of chemically induced mouse skin tumorigenesis. 140: 21-25.
Isnansetyo A., Trijoko & E.P. Setyowati. 2005. Skrining, isolasi dan pemurnian senyawa antibakteri dan antijamur dari rumput laut dan sponge. Laporan Hasil Penelitian Hibah Bersaing XIII/1 Perguruan Tinggi. LPPM UGM. 32 p.
Isnansetyo, A & Y. Kamei. 2003. MC21-A, a bactericidal antibiotic produced by a new marine bacterium, Pseudoalteromonas phenolica sp. nov., against methicillin-resistant Staphylococcus aureus (MRSA). Antimicrob. Agents Chemother. 47: 480-488.
Kerrigen, D & C.C. Suckling. 2018. A meta-analysis of intergrated multitrophic aquaculture: extractive species growth is most successful within close proximity to open-water fish farms. Review in Aquaculture 10 (3): 560-52
Rocha, F.D., A.R. Soares., P. J. Houghton., R.C. Pereira., M.A.C. Kaplan & V.L. Teixeira. 2007. Potential Cytotoxic activity of some Brazilian seaweeds on human melanoma cells. Phytother. Res. 21: 170–175.
Sánchez-Machado, D.I., J. López-Cervantes., J. López- Hernández & P. Paseiro-Losada.. Fatty acids, total lipid, protein and ash contents of processed edible seaweeds. Food Chemistry. 85: 439-444.
Stevan, F.R., M.B. Oliveira., D.F. Bucchi ., Noseda., M. Iacomini & M.E. Duarte . 2001. Cytotoxic effects against HeLa cells of polysaccharides from seaweeds. Journal of Submicroscopic Cytology and Pathology. 33 (4): 477-84.
Teas, J., S. Pino., A. Critchley & L. E. Braverman. 2004. Variability of Iodine Content in Common Edible Seaweeds. Thyroid. 14: 836-841.
Wang, H., E. V. Ooi & P.O. Ang Jr. 2008. Antiviral activities of extracts from Hong Kong seaweeds. Journal of Zhejiang University SCIENCE B. 9 (12): 969-976.
Yan, X., Y. Chuda., M. Suzuki & T. Nagata. 1999. Fucosantin as a major antioxidant in Hijikia fusiformis, a common edible seaweed. Biosci. Biotechnol. Biochem. 63: 605-607.
Zhao, C. Purification and composition analysis of polysaccharide from edible seaweed Enteromorpha prolifera and polysaccharides depolymerized enzymes from microorganisms. Res J Biotechnol 9 (1): 30-36.
DOI: https://doi.org/10.22146/jfs.34103
Article Metrics
Abstract views : 2925
| 
views : 2718
Refbacks
- There are currently no refbacks.
Copyright (c) 2019 Jurnal Perikanan Universitas Gadjah Mada
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Jurnal Perikanan Universitas Gadjah Mada (print ISSN 0853-6384; online ISSN 2502-5066) is published by Department of Fisheries, Universitas Gadjah Mada in collaboration with Semnaskan UGM (Seminar Nasional Tahunan Hasil Perikanan dan Kelautan) and ISMFR (International Symposium on Marine and Fisheries Research).
View My Stats