Toksisitas dan Aktivitas Anti-mikroba Ekstrak Etanol Bunga Karang dari Perairan Pulau Tabuhan Banyuwangi dan Pulau Menjangan Bali Barat

Erna Prawita Setyowati(1*), Umar Anggara Jenie(2), Sudarsono Sudarsono(3), Broto Kardono(4)

(1) Fakultas Farmasi Universitas Gadjah Mada
(2) Fakultas Farmasi Universitas Gadjah Mada
(3) Fakultas Farmasi Universitas Gadjah Mada
(4) Lembaga Ilmu Pengetahuan Indonesia
(*) Corresponding Author


The purpose of the research were to determine the toxicity and antimicrobial activity of ethanolic extract of sponges collected from Tabuhan Island, Banyuwangi and Menjangan Island, West Bali. Extracts were made by maceration of the sponge samples for 3 x 24 hours. The toxicity test was carried out by Brine Shrimp Lethality Test (BST) with 48 hours-old Artemia sp. The effect of ethanolic extract was identified by determining the mortality of Artemia sp. and LC50 of each extract was analyzed by using probit analysis.
Antimicrobial activity was tested by agar diffusion method. The result showed that several ethanolic extracts exhibited toxicity to Artemia sp. The toxicity test showed that extract of sponge code B43 was the most toxic with the LC50 of 8.4 µg/ml. Antimicrobial activity test exhibited that ethanolic extract of sponge with code numbers B2, B57, B61 dan W5 were active against Candida ablicans, while the axtracts of sponge with code numbers B9, B35, B47, B51 (Liosina sp.), B57, B61, B63 and W11 were active against Staphylococcus aureus. One ethanolic extract from sponge with code number B57 exibited activity against Eschericia coli.


antimicrobial, Brine Shrimp Lethality Test, sponge, toxicity

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Castro, P. and M.E. Huber. 1997. Marine biology. Second Edition. Mc. Graw Hill Book Company Inc., New York. 109-112 p.

Garson, M.J. 1994. The Biosynthesis of secondary metabolits: Why is important. In: Sponges in time and space. R.W.M. van Soest, Th. M.G. Van Kempen and J.C Braekman (Eds.). Proceeding 4th International Porifera Conggress, Amsterdam/Netherland.: 428-429.

Hooper, J.N.A., 1997, Guide to sponge collection and identification, Sponge Guide, version March, Quensland Museum, Brisbane.: 33-38.

Meyer, F., Putnam, J. Nichols, and Mc. Laughin. 1982. Brine Shrimp: a convenient general bioassay for active lants constituent. Plant Medica. 45.

Schmitz, F.J., B.F. Bowden, and S.I.Toth. 1993. Antitumor and cytotoxic compounds from marine organisms, in: Marine Biotechnology. D.H. Attaway, and O.R. Zaborsky. (Eds.). Plenum Press. New York and London. 237-260.

Setyowati, E.P., Sudarsono, and S. Wahyuono. 2003. Active fraction from sponge Stylissa flabelliformis collected from Menjangan National Park West Bali. Journal of Technoscience Yogyakarta. 16 (3): 499-513.

Setyowati, E.P., Sudarsono, and S. Wahyuono. 2004. Cytotoxic and antimicrobial test of the bioactive compound isolated from Stylissa flabelliformis sponge. Indonesian Journal of Pharmacy. Yogyakarta. 15 (2): 50-56.

Setyowati, E.P., Sudarsono, and S. Wahyuono. 2005. Jaspamide: structure identification of citotoxic and fungicide compound from Stylissa flabelliformis sponges. Indonesian Journal of Pharmacy. Yogyakarta. 16 (1): 1-5.

Soest, R.W.M. van, 1990, Shallow-water reef sponges of eastern Indonesia. In: New perspectives in sponge biology. K. Rützler. (Ed). Smithsonian Institution. John Press. Washington DC. 302-308


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Jurnal Perikanan Universitas Gadjah Mada (print ISSN 0853-6384; online ISSN 2502-5066) is published by Department of Fisheries, Universitas Gadjah Mada in collaboration with Semnaskan UGM (Seminar Nasional Tahunan Hasil Perikanan dan Kelautan) and ISMFR (International Symposium on Marine and Fisheries Research).


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