PENGARUH PERBEDAAN KANDUNGAN ASAM LEMAK N-3 DALAM PAKAN TERHADAP PENAMPILAN REPRODUKSI IKAN ZEBRA (Brachydanio rerio)

https://doi.org/10.22146/jfs.168

Nur Bambang Priyo Utomo(1*), Ing Mokoginta(2), Lia Nurmalia(3)

(1) Faculty of Fisheries and Marine Science, Bogor Agricultural University
(2) Faculty of Fisheries and Marine Science, Bogor Agricultural University
(3) Faculty of Fisheries and Marine Science, Bogor Agricultural University
(*) Corresponding Author

Abstract


This experiment was conducted to determine the dietary n-3 fatty acid requirement for reproduction of broodstock zebrafish, Brachydanio rerio. Three isonitrogenous (39% crude protein) and isocaloric (3,260 kcal digestible energy/kg diet) practical diets, namely diets A, B, and C, with different levels of fatty acids were fed to zebrafish broodstock. The broodstock were cultivated in aquaria. Diet A contained low dosage of n-3 fatty acids (0.4%) and 2% n-6 fatty acids, while diets B and C contained 2% n-6 fatty acids, combined respectively with 1% and 1.5% n-3 fatty acids. Fish were fed ad satiation for 60 days using these diets. During feeding periode, gonade maturation stages were examined. The n-3 and n-6 fatty acids affected fecundity, fertilization rate, and hatching rate. On the other hand, fish fed on diets A, B, or C did not show any significance differences in the gonade somatic index and total Survival Rate (SR) of larvae produced. Fish fed on diet B produced the highest fecundity (616 eggs/g of fish), fertilization rate (94.6%), and hatching rate (93.8%). The total lipid content of eggs were significant, ranging from B (29.7%), C (23.7%), and A (16.1%). At a dosage of 2% n-6 fatty acids, zebrafish require 1% of dietary n-3 fatty acids in the diet for reproduction. Excess dosage of n-3 fatty acid in the diet adversely affected fecundity, fertilization rate, and hatching rate.


Keywords


Brachydanio rerio, fatty acid, reproductive performance

Full Text:

Full Article (PDF)


References

Fernandez-Palacios, H. Izguerdo, M.S. Robaina, L. Valencia, A. Salhi, and J. Vergara. 1994. Effect of n-3 HUFA level in broodstock diets on egg quality of gilthead sea bream (Sparus aurata L.). Aquaculture 132: 325-337.

Izquierdo, M.S., H. Fernandez-Palacios, and A.G.J. Tacon. 2001. Effect of broodstock nutrition on reproductive performance of fish. Aquaculture. 197: 25-42.

Leray, C., G. Nonnotte, P. Roubaud, and C. Leger. 1985. Incidence of n-3 essential fatty acid efficiency on trout reproductive process. Reproduction Nutrition Development. 25: 256-581.

Maack, G. and H. Segner. 2004. The gonadeal development of the zebrafish (Danio rerio), Ham. Buc. hhtp://www.Igb_berlin.com. Diakses tanggal 25 May 2004.

Sorensen, P.W., T.J. Hara, N.E. Stacey, and F.W. Goetz. 1988. F prosta-glandins function as potent stimulants that comprise the post-ovulatory female sex pheromone in goldfish. Biol. Reprod. 39:1039-1050.

Steel, R.G.D. and J.H. Torrie. 1980. Principles and procedures of statis-tics. Biometrical Approach. Mc Graw-Hill Book Company. 633 p.

Takeuchi, T. 1988. Laboratory work chemical evaluation of dietary nutrients. In: Fish nutrition and mariculture. Watanabe, T. (Ed.). Department of Aquatic Bioscience. Tokyo University of Fisheries. JICA: 179-225.

Watanabe, T., T. Arakawa, C. Kitajima, and S. Fujita. 1984. Effect of nutria-tional quality of broodstock diets on reproduction of red sea bream. Nippon Suisan Gakkasihi. 50: 495.



DOI: https://doi.org/10.22146/jfs.168

Article Metrics

Abstract views : 573 | views : 646

Refbacks

  • There are currently no refbacks.




Copyright (c)



Jurnal Perikanan Universitas Gadjah Mada Indexed by:

 

 

View My Stats